Anti-CCR5 抗体 (ab7346)
Key features and details
- Rabbit polyclonal to CCR5
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-CCR5 antibody
CCR5 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to CCR5 -
由来種
Rabbit -
特異性
This antibody recognises the N-terminal region of the chemokine receptor CCR5. -
アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human CCR5 aa 6-20 (N terminal).
Sequence:SSPIYDINYYTSEPC
(Peptide available asab7876) -
ポジティブ･コントロール
- WB: THP-1 whole cell lysate.
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特記事項
HIV and chemokine receptorThe Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C. -
バッファー
pH: 7.20
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab7346の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
Use a concentration of 20 µg/ml.
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| WB |
1/1000 - 1/2000. Detects a band of approximately 40 kDa.
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| 特記事項 |
|---|
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IHC-P
Use a concentration of 20 µg/ml. |
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WB
1/1000 - 1/2000. Detects a band of approximately 40 kDa. |
ターゲット情報
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機能
Receptor for a number of inflammatory CC-chemokines including MIP-1-alpha, MIP-1-beta and RANTES and subsequently transduces a signal by increasing the intracellular calcium ion level. May play a role in the control of granulocytic lineage proliferation or differentiation. Acts as a coreceptor (CD4 being the primary receptor) for HIV-1 R5 isolates. -
組織特異性
Highly expressed in spleen, thymus, in the myeloid cell line THP-1, in the promyeloblastic cell line KG-1A and on CD4+ and CD8+ T-cells. Medium levels in peripheral blood leukocytes and in small intestine. Low levels in ovary and lung. -
関連疾患
Genetic variation in CCR5 is associated with suseptibility to diabetes mellitus insulin-dependent type 22 (IDDM22) [MIM:612522]. A multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels. -
配列類似性
Belongs to the G-protein coupled receptor 1 family. -
翻訳後修飾
Sulfated on at least 2 of the N-terminal tyrosines. Sulfation contributes to the efficiency of HIV-1 entry and is required for efficient binding of the chemokines, CCL3 and CCL4.
O-glycosylated, but not N-glycosylated. Ser-6 appears to be the major site. Also sialylated glycans present which contribute to chemokine binding. Thr-16 and Ser-17 may also be glycosylated and, if so, with small moieties such as a T-antigen.
Palmitoylation in the C-terminal is important for cell surface expression, and to a lesser extent, for HIV entry.
Phosphorylation on serine residues in the C-terminal is stimulated by binding CC chemokines especially by APO-RANTES. -
細胞内局在
Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 1234 Human
- Omim: 601373 Human
- SwissProt: P51681 Human
- Unigene: 450802 Human
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別名
- AM4 7 antibody
- C C chemokine receptor type 5 antibody
- C C CKR 5 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR5 antibody (ab7346)
Immunohistochemistry of CCR5 in human lymph node tissue with ab7346 at 2.5 μg/ml.
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Western blot - Anti-CCR5 antibody (ab7346)Anti-CCR5 antibody (ab7346) at 1/1000 dilution + THP-1 whole cell lysate
Observed band size: 40 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR5 antibody (ab7346)Immunofluorescence of CCR5 in human lymph node tissue with ab7346 at 20 μg/mL.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR5 antibody (ab7346)ab7346 at 20µg/ml staining CCR5 in human lymph node tissue by IHC
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (7)
ab7346 は 7 報の論文で使用されています。
- Watzling M et al. Three-Dimensional Breast Cancer Model to Investigate CCL5/CCR1 Expression Mediated by Direct Contact between Breast Cancer Cells and Adipose-Derived Stromal Cells or Adipocytes. Cancers (Basel) 15:N/A (2023). PubMed: 37444610
- Chengcheng L et al. Identification of the Immune-Related Genes in Tumor Microenvironment That Associated With the Recurrence of Head and Neck Squamous Cell Carcinoma. Front Cell Dev Biol 9:723721 (2021). PubMed: 34490269
- Mikhailova A et al. Antiapoptotic Clone 11-Derived Peptides Induce In Vitro Death of CD4+ T Cells Susceptible to HIV-1 Infection. J Virol 94:N/A (2020). PubMed: 32350074
- Kuzmina A et al. Functional Mimetics of the HIV-1 CCR5 Co-Receptor Displayed on the Surface of Magnetic Liposomes. PLoS One 10:e0144043 (2015). PubMed: 26629902
- Jiang AP et al. Human Mucosal Mast Cells Capture HIV-1 and Mediate Viral trans-Infection of CD4+ T Cells. J Virol 90:2928-37 (2015). PubMed: 26719250
- Levina V et al. Role of eotaxin-1 signaling in ovarian cancer. Clin Cancer Res 15:2647-56 (2009). IHC-P ; Human . PubMed: 19351767
- Dittmer A et al. Human mesenchymal stem cells induce E-cadherin degradation in breast carcinoma spheroids by activating ADAM10. Cell Mol Life Sci 66:3053-65 (2009). WB ; Human . PubMed: 19603142