Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
リコンビナントRabMAb

Anti-CCR3 抗体 [Y31] (ab32512)

Reviews (1)Q&A (3)References (23)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR3 antibody [Y31] (ab32512)
  • Anti-CCR3 antibody [Y31] (ab32512)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [Y31] to CCR3
  • Suitable for: IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-CCR3 antibody [Y31]
    CCR3 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [Y31] to CCR3

  • 由来種

    Rabbit

  • 特異性

    We do not guarantee IHC-P for mouse and rat.

  • アプリケーション

    適用あり: IHC-Pmore details
    適用なし: Flow Cyt,ICC/IF,IP or WB

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat, Macaque monkey

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • K562 and Daudi cell lysates and human liver tissue.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab32512の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
特記事項
IHC-P
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
追加情報
Is unsuitable for Flow Cyt,ICC/IF,IP or WB.

ターゲット情報

  • 機能

    Receptor for a C-C type chemokine. Binds to eotaxin, eotaxin-3, MCP-3, MCP-4, RANTES and MIP-1 delta. Subsequently transduces a signal by increasing the intracellular calcium ions level. Alternative coreceptor with CD4 for HIV-1 infection.

  • 組織特異性

    In eosinophils as well as trace amounts in neutrophils and monocytes.

  • 配列類似性

    Belongs to the G-protein coupled receptor 1 family.

  • 細胞内局在

    Cell membrane.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • B chemokine receptor antibody
    • b-chemokine receptor antibody
    • C C chemokine receptor type 3 antibody
    • C C CKR 3 antibody
    • C-C chemokine receptor type 3 antibody
    • C-C CKR-3 antibody
    • CC chemokine receptor 3 antibody
    • CC chemokine receptor type 3 antibody
    • CC CKR 3 antibody
    • CC R3 antibody
    • CC-CKR-3 antibody
    • CCCKR3 antibody
    • CCR 3 antibody
    • CCR-3 antibody
    • CCR3 antibody
    • CCR3_HUMAN antibody
    • CD 193 antibody
    • CD193 antibody
    • CD193 antigen antibody
    • Chemokine (C C motif) receptor 3 antibody
    • chemokine (C-C motif) receptor 3 antibody
    • Chemokine (CC) receptor 1 like 2 antibody
    • Chemokine CC motif receptor 3 antibody
    • Chemokine receptor 3 antibody
    • CK R3 antibody
    • CKR 3 antibody
    • CKR3 antibody
    • CMKB R3 antibody
    • CMKBR 3 antibody
    • CMKBR1L2 antibody
    • CMKBR3 antibody
    • Eosinophil CC chemokine receptor 3 antibody
    • Eosinophil eotaxin receptor antibody
    • Eotaxin receptor antibody
    • Macrophage inflammatory protein 1 alpha receptor like 2 antibody
    • MGC102841 antibody
    • MIP1 Alpha RL2 antibody
    see all

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR3 antibody [Y31] (ab32512)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR3 antibody [Y31] (ab32512)

    Immunohistochemical analysis of paraffin-embedded human liver tissue using ab32512 at 1/100 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Anti-CCR3 antibody [Y31] (ab32512)
    Anti-CCR3 antibody [Y31] (ab32512)

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (23)

ab32512 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab32512 は 23 報の論文で使用されています。

  • Xu H  et al. Cancer associated fibroblast-derived CCL5 promotes hepatocellular carcinoma metastasis through activating HIF1α/ZEB1 axis. Cell Death Dis 13:478 (2022). PubMed: 35589690
  • Feng SY  et al. Increased joint loading induces subchondral bone loss of the temporomandibular joint via the RANTES-CCRs-Akt2 axis. JCI Insight 7:N/A (2022). PubMed: 36173680
  • Yu WY  et al. Acupuncture Alleviates Menstrual Pain in Rat Model via Suppressing Eotaxin/CCR3 Axis to Weak EOS-MC Activation. Evid Based Complement Alternat Med 2022:4571981 (2022). PubMed: 35069759
  • Guérard A  et al. The Chemokine Receptor CCR3 Is Potentially Involved in the Homing of Prostate Cancer Cells to Bone: Implication of Bone-Marrow Adipocytes. Int J Mol Sci 22:N/A (2021). PubMed: 33671469
  • Li X  et al. miR-30a-3p participates in the development of asthma by targeting CCR3. Open Med (Wars) 15:483-491 (2020). PubMed: 33313407
View all Publications for this product

レビューと Q&A

Show All レビュー Q&A
レビューを送る 質問を送る

1-4 of 4 Abreviews or Q&A

Western blot abreview for Anti-CCR3 antibody [Y31]

 Good
Abreviews
This product is known to not work in this application or species.
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Jurkat cells)
Loading amount
100000 cells
Specification
Jurkat cells
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 May 25 2010

Question

To confirm that there was not a problem with the Westerns themselves, we re-probed the CCR2 membrane with a rabbit monoclonal antibody against LRP-1. I've attached a power point slide of the image- as you can see, the blot looked fine for LRP-1 indicating that our transfer and secondary are working properly. If abcam refunds 100%, then we will take the refund. .
Thanks,

Read More
Answer

Thank you very much for your reply and for sending this additional information.

Your credit note ID is ***.

I am sorry that these antibodies did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask your purchasing department to contact our accounting department at mailto:us.credits@abcam.com so that we may gather the correct information needed for the refund. Please refer to the credit note ID in any correspondence with our accounting department.

I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require furtherassistance or advice.

Read More

Question

Inquiry: I am having trouble getting signal using your CCR2 (ab32144, lot GR52022-3), CCR3 (ab32512, lot GR52022-3), and CCR5 (ab32048, lot GR40445-5) antibodies. Here are my Western Blotting conditions: 1. Loaded 75, 50, 30, 20, and 10 micrograms per well of mouse spleen extract under both reducing and non-reducing conditions on a 4-12% Bis-tris gel from Invitrogen. MES buffer was used for the run. 2. Proteins were transferred onto a nitrocellulose membrane using an iBlot system (Invitrogen). 3. Membrane was blocked for 1 hour RT in 5% milk 4. Primary incubation for all three antibodies was overnight (approx. 16 hours) at 4 C. Primary antibodies were diluted 1:500 in 5% milk. 5. Secondary incubation was using goat anti-rabbit from santa cruz (1:5,000) for 1 hour RT. 6. Membrane was washed following both primary and secondary incubations three times in PBS-T. 7. West Pico was used to develop the blot, and bands were visualized using an ImageQuant LAS4000, and exposures were for 30 minutes. Our primary goal is to quantify changes in CCR2, 3, and 5 in brain and brain endothelial cells, however, to optimize antibody conditions, we typically use some other tissue type as a positive control. For CCRs, spleen seemed suitable. We see very a very faint band for CCR2 which appears to be at the correct molecular weight, but IR is very weak. We did not detect any bands for CCR5, and the bands observed for CCR3 were not the correct molecular weight. That all three antibodies did not work as expected is of concern to me. I'd appreciate your assistance with this issue.

Read More
Answer

Thank you very much for contacting us and letting us know about the trouble with these antibodies.

Thank you also for sending so many details of your protocol.The protocol seems to be ok and similar to the protocol that the lab uses to test this antibody, and I would expect to see bands at the correct molecular weights under these conditions. Does the anti-rabbit secondary antibody work with other primary antibodies? Was protein transfer confirmed with a Ponceau stain or some other method? If you probed for a loading control like actin, what were the results?

We do fully guarantee these antibodies to work in Western blot with mouse samples, so if necessary I can send replacements or issue credits or refunds.

I look forward to hearing from you. Please let me know if you have any questions or if there is anything else that we can do for you.

Read More

Question

Dear technical team,

Our customer will perform IHC-p so she wants to know which antigen retrieval buffer is good for her sample.
Please recommend specific buffer for citric buffer pH 6.0 or Tris pH 9.0

Best regards

Read More
Answer

Thank you for your inquiry.

In general we recommend to use EDTA pH9. This antigen retrieval method is optimal for the most antigens. Please see the attachment and this publication (Emoto et al., J Histochem Cytochem. 2005 Nov ;53 (11) : 1311-21.) for more information.

ab28775 was used successfully by our customers without, with citrate buffer pH6 and with Tris pH10. Please see Abreviews on the datasheet.

The image on the datasheetfor ab32512 was generated with citrate buffer pH6.

The optimal protocol usually has to be determined by the end user individually.

I hope this information is helpful and wish your customer good luck with their experiments.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com