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リコンビナントRabMAb

Anti-CCL27 抗体 [EPR8582(2)] (ab186421)

Submit a review Submit a question References (4)
Immunocytochemistry/ Immunofluorescence - Anti-CCL27 antibody [EPR8582(2)] (ab186421)
  • Western blot - Anti-CCL27 antibody [EPR8582(2)] (ab186421)
  • Flow Cytometry (Intracellular) - Anti-CCL27 antibody [EPR8582(2)] (ab186421)
  • Anti-CCL27 antibody [EPR8582(2)] (ab186421)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8582(2)] to CCL27
  • Suitable for: Flow Cyt (Intra), WB, ICC/IF
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-CCL27 antibody [EPR8582(2)]
    CCL27 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR8582(2)] to CCL27

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Flow Cyt (Intra), WB, ICC/IFmore details

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • Human CCL27 recombinant protein; A431 and HACAT cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR8582(2)

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab186421の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
1/320.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000 - 1/10000. Detects a band of approximately 10 kDa (predicted molecular weight: 13 kDa).
ICC/IF
1/250.
特記事項
Flow Cyt (Intra)
1/320.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000 - 1/10000. Detects a band of approximately 10 kDa (predicted molecular weight: 13 kDa).
ICC/IF
1/250.

ターゲット情報

画像

  • Immunocytochemistry/ Immunofluorescence - Anti-CCL27 antibody [EPR8582(2)] (ab186421)
    Immunocytochemistry/ Immunofluorescence - Anti-CCL27 antibody [EPR8582(2)] (ab186421)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed A431 cells labeling CCL27 with ab186421 at 1/250 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at 1/200 dilution. Counter stained with Dapi.

  • Western blot - Anti-CCL27 antibody [EPR8582(2)] (ab186421)
    Western blot - Anti-CCL27 antibody [EPR8582(2)] (ab186421)
    Anti-CCL27 antibody [EPR8582(2)] (ab186421) at 1/5000 dilution + Human CCL27 recombinant protein at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 13 kDa

  • Flow Cytometry (Intracellular) - Anti-CCL27 antibody [EPR8582(2)] (ab186421)
    Flow Cytometry (Intracellular) - Anti-CCL27 antibody [EPR8582(2)] (ab186421)

    Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed HACAT cells labeling CCL27 with ab186421 at 1/320 dilution (red) compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution. 

     

     

  • Anti-CCL27 antibody [EPR8582(2)] (ab186421)
    Anti-CCL27 antibody [EPR8582(2)] (ab186421)

参考文献 (4)

ab186421 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab186421 は 4 報の論文で使用されています。

  • Fu X  et al. Qi-Long-Tian formula extract alleviates symptoms of acute high-altitude diseases via suppressing the inflammation responses in rat. Respir Res 22:52 (2021). PubMed: 33579290
  • Tao B  et al. CYB561D2 up-regulation activates STAT3 to induce immunosuppression and aggression in gliomas. J Transl Med 19:338 (2021). PubMed: 34372858
  • Su Q  et al. Bilateral Paraventricular Nucleus Upregulation of Extracellular Superoxide Dismutase Decreases Blood Pressure by Regulation of the NLRP3 and Neurotransmitters in Salt-Induced Hypertensive Rats. Front Pharmacol 12:756671 (2021). PubMed: 34899311
  • Li S  et al. HOXC10 promotes proliferation and invasion and induces immunosuppressive gene expression in glioma. FEBS J 285:2278-2291 (2018). PubMed: 29676849

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