AB181323

Anti-CCL27 抗体 [EPR17284-217]

Anti-CCL27 antibody [EPR17284-217]

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal CCL27 antibody. Suitable for IP, WB and reacts with Mouse samples. Cited in 1 publication.

別名を表示する

Ilc, Scya27, Ccl27, C-C motif chemokine 27, CC chemokine ILC, Cutaneous T-cell-attracting chemokine, ESkine, IL-11 R-alpha-locus chemokine, Skinkine, Small-inducible cytokine A27, CTACK, ALP, mILC

2 Images

Supplier Data

Immunoprecipitation - Anti-CCL27 antibody [EPR17284-217] (AB181323)

CCL27 was immunoprecipitated from 0.35 mg of mouse skin lysate with ab181323 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab181323 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1 : Mouse skin lysate 10 μg (Input).

Lane 2 : ab181323 IP in mouse skin lysate (+).

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab181323 in mouse skin lysate (-).

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 minutes.

All lanes:

Immunoprecipitation - Anti-CCL27 antibody [EPR17284-217] (ab181323)

Predicted band size: 13 kDa

false

Supplier Data

Western blot - Anti-CCL27 antibody [EPR17284-217] (AB181323)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-CCL27 antibody [EPR17284-217] (ab181323) at 1/1000 dilution

All lanes:

Mouse skin lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 13 kDa

Observed band size: 13 kDa

true

Exposure time: 5s

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR17284-217

アイソタイプ

IgG

キャリアフリー

交差種

Mouse

アプリケーション

WB, IP

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }

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製品の詳細

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

CCL27 also known as CTACK (cutaneous T cell-attracting chemokine) is a chemokine that plays a critical role in skin immune surveillance. It has a mass of approximately 10 kDa. CCL27 is expressed in keratinocytes which are the predominant cells in the epidermis. It is also secreted by cells in inflamed skin. Researchers often use ELISA (enzyme-linked immunosorbent assay) techniques to study CCL27 due to its importance in skin immunology.

Biological function summary

CCL27 attracts T cells to the skin's surface facilitating immune responses. Although not part of a larger complex CCL27 interacts with its receptor CCR10 on T cells directing them to skin sites where they can perform immune surveillance and respond to pathogens. This chemokine-receptor interaction is essential for maintaining skin homeostasis and effective immune responses.

Pathways

The role of CCL27 gets significant attention in the CCR10-CTACK axis which is pivotal in the cutaneous immune system's functioning. Additionally the CCL27/CCR10 pathway has connections to the wider chemokine signaling network that regulates leukocyte trafficking. Through these associations CCL27 is connected to other chemokines and receptors including CCL28 which also shares CCR10 as a receptor highlighting the integrated nature of chemokine function in immune pathways.

CCL27 has been linked to various skin conditions such as psoriasis and atopic dermatitis. In psoriasis CCL27 recruits CCR10-expressing T cells to the skin aggravating the inflammatory response. Similarly in atopic dermatitis increased CCL27 levels may contribute to disease pathology by attracting immune cells that perpetuate inflammation. Understanding the interaction between CCL27 and these conditions may offer insight into potential therapeutic targets aimed at modulating chemokine signaling to alleviate symptoms.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Chemotactic factor that attracts skin-associated memory T-lymphocytes. May play a role in mediating homing of lymphocytes to cutaneous sites. May play a role in cell migration during embryogenesis. Nuclear forms may facilitate cellular migration by inducing cytoskeletal relaxation. Binds to CCR10.

See full target information Ccl27

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Experimental and therapeutic medicine 25:240 PubMed37114177

2023

Tyrosol attenuates lipopolysaccharide‑induced inflammation in HUVECs to promote vascular health against atherosclerosis challenge.

Applications

Unspecified application

Species

Unspecified reactive species

Weiwei Zhao,Huizhen Wei,Jun Lu,Wenjun Sha,Dusang Sun,Ting Pan,Tao Lei

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CCL27 抗体 [EPR17284-217]

Anti-CCL27 antibody [EPR17284-217]

Immunoprecipitation - Anti-CCL27 antibody [EPR17284-217] (AB181323)

Western blot - Anti-CCL27 antibody [EPR17284-217] (AB181323)

関連する標識済み抗体及び組成の異なる製品 (2)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (1)

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組合せ製品

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Abcam product promise