Anti-CCL18 抗体 [EPR24781-84] (BSA and Azide free) (ab300058)

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Western blot - Anti-CCL18 antibody [EPR24781-84] (BSA and Azide free) (AB300058)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR24781-84] to CCL18 - BSA and Azide free
Suitable for: WB, Dot blot, IHC-P
Reacts with: Human

Alexa Fluor® 488 Alexa Fluor® 647 Unconjugated

製品名

Anti-CCL18 antibody [EPR24781-84] (BSA and Azide free)
CCL18 一次抗体製品一覧
製品の詳細

Rabbit monoclonal [EPR24781-84] to CCL18 - BSA and Azide free
由来種

Rabbit
アプリケーション

適用あり: WB, Dot blot, IHC-Pmore details
適用なし: Flow Cyt (Intra),ICC/IF or IP
種交差性

交差種: Human
非交差種: Mouse, Rat
免疫原

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
ポジティブ･コントロール
- WB: THP-1 and THP-1 treated with 0.1 µg/ml PMA for 48 hours, then with 0.045 µg/ml IL-4 added for 72 hours, whole cell lysates. IHC-P: Human tonsil, and HEK-293T cells transfected with human C-C motif chemokine 18 expression vector containing a myc-His tag cell lysates. Human stomach cancer tissues. DB: CCL18 target protein
特記事項
ab300058 is a carrier free version of ab300057.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

製品の状態

Liquid
保存方法

Shipped at 4°C. Store at +4°C.
バッファー

pH: 7.2
Constituent: 100% PBS
キャリア・フリー

はい
Concentration information loading...
精製度

Protein A purified
ポリ/モノ

モノクローナル
クローン名

EPR24781-84
アイソタイプ

IgG
研究分野

Alternative Versions
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (HRP) (ab97051)

The Abpromise guarantee

Abpromise保証は、次のテスト済みアプリケーションにおけるab300058の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション	Abreviews	特記事項
WB		Use at an assay dependent concentration. Detects a band of approximately 10 kDa (predicted molecular weight: 9 kDa).
Dot blot		Use at an assay dependent concentration.
IHC-P		Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

特記事項
WB Use at an assay dependent concentration. Detects a band of approximately 10 kDa (predicted molecular weight: 9 kDa).
Dot blot Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

追加情報

Is unsuitable for Flow Cyt (Intra),ICC/IF or IP.

機能

Chemotactic factor that attracts lymphocytes but not monocytes or granulocytes. May be involved in B-cell migration into B-cell follicles in lymph nodes. Attracts naive T-lymphocytes toward dendritic cells and activated macrophages in lymph nodes, has chemotactic activity for naive T-cells, CD4+ and CD8+ T-cells and thus may play a role in both humoral and cell-mediated immunity responses.
組織特異性

Expressed at high levels in lung, lymph nodes, placenta, bone marrow, dendritic cells present in germinal centers and T-cell areas of secondary lymphoid organs and macrophages derived from peripheral blood monocytes. Not expressed by peripheral blood monocytes and a monocyte-to-macrophage differentiation is a prerequisite for expression. Expressed in synovial fluids from patients with rheumatoid and septic arthritis and in ovarian carcinoma ascitic fluid.
配列類似性

Belongs to the intercrine beta (chemokine CC) family.
細胞内局在

Secreted.
Target information above from: UniProt accession P55774 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
参照データベース
- Entrez Gene: 6362 Human
- Omim: 603757 Human
- SwissProt: P55774 Human
- Unigene: 143961 Human
別名
- Alternative macrophage activation associated CC chemokine 1 antibody
- Alternative macrophage activation-associated CC chemokine 1 antibody
- AMAC-1 antibody
- AMAC1 antibody
- CC chemokine PARC antibody
- CCL18 antibody
- CCL18(4-69) antibody
- CCL18_HUMAN antibody
- CKb7 antibody
- DC-CK1 antibody
- DCCK1 antibody
- DCCK1 antibody
- Dendritic cell chemokine 1 antibody
- Macrophage inflammatory protein 4 antibody
- MIP-4 antibody
- MIP4 antibody
- PARC antibody
- Pulmonary and activation regulated chemokine antibody
- Pulmonary and activation-regulated chemokine antibody
- SCYA18 antibody
- Small inducible cytokine A18 antibody
- Small inducible cytokine A18 antibody
- Small-inducible cytokine A18 antibody
see all

Western blot - Anti-CCL18 antibody [EPR24781-84] (BSA and Azide free) (AB300058)

All lanes : Anti-CCL18 antibody [EPR24781-84] (ab300057) at 1/1000 dilution

Lane 1 : THP-1( Human monocytic leukemia monocyte) treated with 0.1µg/ml PMA for 48 hours whole cell lysate
Lane 2 : THP-1 treated with 0.1µg/ml PMA for 48 hours, then with 0.045 µg/ml IL-4 added for 72 hours, whole cell lysate

Lysates/proteins at 50 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 9 kDa
Observed band size: 10 kDa why is the actual band size different from the predicted?

Exposure time: 103 seconds

This data was developed using ab300057, the same antibody clone in a different buffer formulation.

5% NFDM/TBST was used as blocking and diluting buffer and concentration.

THP-1 cells after PMA and IL-4 treatment secrete high levels of CCL18. (PMID: 25502147).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCL18 antibody [EPR24781-84] (BSA and Azide free) (AB300058)

This data was developed using ab300057, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labelling CCL18 with ab300057 at 1/500 (0.982 µg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Positive staining on human tonsil. The section was incubated with ab300057 at 4°C overnight. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCL18 antibody [EPR24781-84] (BSA and Azide free) (AB300058)

This data was developed using ab300057, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded A HEK-293T cells transfected with human C-C motif chemokine 18 expression vector containing a myc-his tag, B: HEK-293T cells untreated labeling labelling CCL18 with ab300057 at 1/200 (2.455 µg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Cytoplasmic staining on HEK-293T cells transfected with a human C-C motif chemokine 18 expression vector containing a myc-His tag (image A). No staining on HEK-293T cells untreated (image B). The section was incubated with ab300057 at 4°C overnight. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCL18 antibody [EPR24781-84] (BSA and Azide free) (AB300058)

This data was developed using ab300057, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human stomach cancer tissue labelling CCL18 with ab300057 at 1/500 (0.982 µ/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Positive staining on human stomach cancer. The section was incubated with ab300057 at 4°C overnight. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Dot Blot - Anti-CCL18 antibody [EPR24781-84] (BSA and Azide free) (AB300058)

This data was developed using ab300057, the same antibody clone in a different buffer formulation.

Dot blot analysis of CCL18 using ab300057 at 1:1000 (0.491 µg/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1:100,000 dilution.

Exposure time: 5.5 seconds

Blocking and diluting buffer and concentration: 5% NFDM/TBST
Dot Blot - Anti-CCL18 antibody [EPR24781-84] (BSA and Azide free) (AB300058)

This data was developed using ab300057, the same antibody clone in a different buffer formulation.

Dot blot analysis of CCL18 using ab300057 at 1:1000 (0.491 µg/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1:100,000 dilution.

Exposuse time: 3 minutes

Blocking and diluting buffer and concentration: 5% NFDM/TBST
Anti-CCL18 antibody [EPR24781-84] (BSA and Azide free) (AB300058)

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheet download

Download

ab300058 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab300058 は論文での使用が確認できていません。

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Key features and details

Related conjugates and formulations

製品の概要

製品名

製品の詳細

由来種

アプリケーション

種交差性

免疫原

ポジティブ･コントロール

特記事項

製品の特性

製品の状態

保存方法

バッファー

キャリア・フリー

精製度

ポリ/モノ

クローン名

アイソタイプ

研究分野

関連製品

Alternative Versions

Compatible Secondaries

アプリケーション

The Abpromise guarantee

ターゲット情報

機能

組織特異性

配列類似性

細胞内局在

参照データベース

別名

画像

プロトコール

データシートおよび資料

Datasheet download

Certificate of Compliance

参考文献 (0)

レビューと Q&A