Anti-CCDC52 抗体 (ab117801)
Key features and details
- Rabbit polyclonal to CCDC52
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-CCDC52 antibody -
製品の詳細
Rabbit polyclonal to CCDC52 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Human
交差が予測される動物種: Rabbit, Horse, Cow, Dog, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
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免疫原
Synthetic peptide, corresponding to a region between amino acids 805-855 of Human CCDC52 (NP_653319.1).
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ポジティブ･コントロール
- HeLa and 293T whole cell lysates.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7-8 -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab117801 was affinity purified using an epitope specific to CCDC52 immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab117801の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/2000 - 1/10000. Predicted molecular weight: 96 kDa.
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| IP |
Use at 2-10 µg/mg of lysate.
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| 特記事項 |
|---|
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WB
1/2000 - 1/10000. Predicted molecular weight: 96 kDa. |
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IP
Use at 2-10 µg/mg of lysate. |
ターゲット情報
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機能
Regulator required for centriole duplication, for proper bipolar spindle formation and chromosome congression in mitosis. -
細胞内局在
Cytoplasm > cytoskeleton > centrosome > centriole. Cytoplasm > cytoskeleton > spindle. - Information by UniProt
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参照データベース
- Entrez Gene: 152185 Human
- Entrez Gene: 100172308 Orangutan
- Omim: 613447 Human
- SwissProt: Q8N0Z3 Human
- SwissProt: Q5RBY6 Orangutan
- Unigene: 477144 Human
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別名
- Coiled coil domain containing 52 antibody
- Coiled coil domain containing protein 52 antibody
- Coiled-coil domain-containing protein 52 antibody
see all
画像
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Western blot - Anti-CCDC52 antibody (ab117801)All lanes : Anti-CCDC52 antibody (ab117801) at 0.1 µg/ml
Lane 1 : 293T whole cell lysate at 50 µg
Lane 2 : 293T whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 96 kDa
Exposure time: 3 minutes -
Immunoprecipitation - Anti-CCDC52 antibody (ab117801)Lane 1: ab1177801 at 1 µg/ml staining CCDC52 in 293T cell lysate immunoprecipitated using ab117801 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Lane 2: control IgG. Predicted band size : 96 kDa. Exposure time: 10 seconds
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab117801 は論文での使用が確認できていません。