Anti-Cbx8 抗体 (ab70796)
Key features and details
- Rabbit polyclonal to Cbx8
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Cbx8 antibody
Cbx8 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Cbx8 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Xenopus tropicalis
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免疫原
Synthetic peptide corresponding to a region between residue 350 and the C-terminus (residue 389) of human Cbx8 (NP_065700.1).
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ポジティブ･コントロール
- HeLa and 293T whole cell lysates.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab70796 was affinity purified using an epitope specific to Cbx8 immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab70796の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/2000 - 1/10000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).
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| IP |
Use at 2-5 µg/mg of lysate.
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| 特記事項 |
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WB
1/2000 - 1/10000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa). |
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IP
Use at 2-5 µg/mg of lysate. |
ターゲット情報
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機能
Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. -
配列類似性
Contains 1 chromo domain. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 506458 Cow
- Entrez Gene: 57332 Human
- Entrez Gene: 30951 Mouse
- Entrez Gene: 303731 Rat
- SwissProt: Q9HC52 Human
- SwissProt: Q9QXV1 Mouse
- Unigene: 387258 Human
- Unigene: 99953 Mouse
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別名
- Cbx8 antibody
- CBX8_HUMAN antibody
- Chromobox Homolog 8 (Drosophila Pc Class) antibody
see all
画像
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Western blot - Anti-Cbx8 antibody (ab70796)All lanes : Anti-Cbx8 antibody (ab70796) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : HEK293T whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 43 kDa -
Immunoprecipitation - Anti-Cbx8 antibody (ab70796)Detection of Cbx8 by Western Blot of Immunoprecipitate.
ab70796 at 1µg/ml staining Cbx8 in HeLa whole cell lysate immunoprecipitated using ab70796 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 3 minutes.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab70796 は 3 報の論文で使用されています。
- Liu Q et al. CBX8 Promotes Cell Proliferation and Metastasis and Leads to Radiotherapy Tolerance of Glioma Cells. Turk Neurosurg 33:46-52 (2023). PubMed: 35713250
- Zeng F et al. KPNA2 interaction with CBX8 contributes to the development and progression of bladder cancer by mediating the PRDM1/c-FOS pathway. J Transl Med 19:112 (2021). PubMed: 33731128
- Oza J et al. A Novel Role of Chromodomain Protein CBX8 in DNA Damage Response. J Biol Chem 291:22881-22893 (2016). PubMed: 27555324