AB259848

Anti-Cbx6 抗体 [EPR23828-108]

Anti-Cbx6 antibody [EPR23828-108]

20ul selling size
RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal CBX6 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human, Rat samples. Cited in 1 publication.

別名を表示する

Chromobox protein homolog 6, CBX6

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cbx6 antibody [EPR23828-108] (AB259848)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cbx6 antibody [EPR23828-108] (AB259848)

Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling Cbx6 with ab259848 at 1/100 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Nuclear staining on human cerebrum (PMID : 24260522). Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cbx6 antibody [EPR23828-108] (AB259848)

Immunohistochemical analysis of paraffin-embedded human colon tissue labeling Cbx6 with ab259848 at 1/100 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Nuclear staining on the immune cells of human colon. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cbx6 antibody [EPR23828-108] (AB259848)

Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma tissue labeling Cbx6 with ab259848 at 1/100 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Nuclear staining on human hepatocellular carcinoma (PMID : 28122351). Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cbx6 antibody [EPR23828-108] (AB259848)

Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling Cbx6 with ab259848 at 1/100 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Nuclear staining on rat cerebrum. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cbx6 antibody [EPR23828-108] (AB259848)

Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling Cbx6 with ab259848 at 1/100 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Nuclear staining on mouse cerebrum. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

Lab

Western blot - Anti-Cbx6 antibody [EPR23828-108] (AB259848)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

Exposure time : 3 minutes

All lanes:

Western blot - Anti-Cbx6 antibody [EPR23828-108] (ab259848) at 1/1000 dilution

Lane 1:

HEK-293T (human embryonic kidney epithelial cell) whole cell lysate at 40 µg

Lane 2:

Human colon tissue lysate at 40 µg

Lane 3:

Human testis tissue lysate at 40 µg

Lane 4:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 40 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 44 kDa

Observed band size: 55 kDa

false

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR23828-108

アイソタイプ

IgG

キャリアフリー

交差種

Mouse, Rat, Human

アプリケーション

WB, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "ChIP-species-checked": "notRecommended", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "ChIP-species-checked": "notRecommended", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "ChIP-species-checked": "notRecommended", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "" } } }

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製品の詳細

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Cbx6 also known as Chromobox Protein Homolog 6 is a component of the Polycomb Group (PcG) proteins with a core function in transcriptional repression. It has a molecular mass of approximately 51 kDa. The primary expression of Cbx6 occurs in stem and progenitor cells but it is also present in various tissues during embryogenesis. The protein contains a chromodomain that facilitates its interaction with histones and contributes to chromatin structure alteration impacting gene expression regulation.

Biological function summary

Cbx6 plays a significant role in maintaining cellular pluripotency and regulating differentiation processes. As part of the Polycomb Repressive Complex 1 (PRC1) Cbx6 participates in modifying chromatin to silence target genes. This complex works synergistically with other PcG proteins to ensure correct temporal and spatial expression of developmentally important genes. The association with histone H3 lysine 27 trimethylation (H3K27me3) is fundamental to its repressive function.

Pathways

Cbx6 functions integrally within the epigenetic regulation pathway and impacts the Wnt signaling pathway. By interacting with other PRC1 members Cbx6 facilitates transcriptional silencing required for the maintenance of stem cell identity. Its activities are closely linked to the modulation of canonical Wnt signaling and in doing so it affects proteins such as β-catenin as well as interacting with other PcG components like Cbx7 and Bmi1.

Cbx6 shows associations with various cancers and developmental disorders. Its dysregulation is observed in malignancies like colorectal cancer where abnormal Cbx6 expression affects the Wnt signaling pathway leading to enhanced proliferation. Furthermore disorders in embryonic development can arise from improper Cbx6 function in Polycomb-mediated gene silencing. Within the context of these conditions Cbx6 works alongside other regulators such as EZH2 in the PRC2 complex highlighting its multifaceted role in disease states.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development (PubMed : 21282530). PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. Possibly contributes to the target selectivity of the PRC1 complex by binding specific regions of chromatin (PubMed : 18927235). Recruitment to chromatin might occur in an H3K27me3-independent fashion (By similarity). May have a PRC1-independent function in embryonic stem cells (By similarity).

See full target information CBX6

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

BMC cancer 23:641 PubMed37430195

2023

An integrative bioinformatics investigation and experimental validation of chromobox family in diffuse large B-cell lymphoma.

Applications

Unspecified application

Species

Unspecified reactive species

Fenling Zhou,Lu Chen,Peng Lu,Yuli Cao,Cuilan Deng,Gexiu Liu

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Cbx6 抗体 [EPR23828-108]

Anti-Cbx6 antibody [EPR23828-108]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cbx6 antibody [EPR23828-108] (AB259848)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cbx6 antibody [EPR23828-108] (AB259848)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cbx6 antibody [EPR23828-108] (AB259848)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cbx6 antibody [EPR23828-108] (AB259848)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cbx6 antibody [EPR23828-108] (AB259848)

Western blot - Anti-Cbx6 antibody [EPR23828-108] (AB259848)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (1)

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise