Anti-CBX1 / HP1 beta 抗体 [MAC353] (ab10811)
Key features and details
- Rat monoclonal [MAC353] to CBX1 / HP1 beta
- Suitable for: WB, ICC/IF, IP, ELISA, IHC-Fr, ChIP
- Knockout validated
- Reacts with: Mouse, Human
- Isotype: IgG2b
製品の概要
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製品名
Anti-CBX1 / HP1 beta antibody [MAC353]
CBX1 / HP1 beta 一次抗体 製品一覧 -
製品の詳細
Rat monoclonal [MAC353] to CBX1 / HP1 beta -
由来種
Rat -
特異性
Ab10811 recognises the M31 molecule in mouse and the homologous HP1 HS beta molecule in man. -
アプリケーション
適用あり: WB, ICC/IF, IP, ELISA, IHC-Fr, ChIPmore details -
種交差性
交差種: Mouse, Human -
免疫原
Fusion protein corresponding to Mouse CBX1/ HP1 beta (C terminal).
Database link: P83917 -
ポジティブ・コントロール
- Murine and human nuclear extracts.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.09% Sodium azide
Constituent: 5% BSA -
Concentration information loading...
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精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
MAC353 -
アイソタイプ
IgG2b -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab10811の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (1) |
Use at an assay dependent concentration. Detects a band of approximately 26 kDa (predicted molecular weight: 22.2 kDa).
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ICC/IF | (1) |
Use at an assay dependent concentration.
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IP |
Use at an assay dependent concentration.
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ELISA |
Use at an assay dependent concentration.
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IHC-Fr |
Use at an assay dependent concentration.
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ChIP |
Use at an assay dependent concentration.
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特記事項 |
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WB
Use at an assay dependent concentration. Detects a band of approximately 26 kDa (predicted molecular weight: 22.2 kDa). |
ICC/IF
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. |
ELISA
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
ChIP
Use at an assay dependent concentration. |
ターゲット情報
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機能
Component of heterochromatin. Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. Interaction with lamin B receptor (LBR) can contribute to the association of the heterochromatin with the inner nuclear membrane. -
組織特異性
Expressed in all adult and embryonic tissues. -
配列類似性
Contains 2 chromo domains. -
翻訳後修飾
Not phosphorylated.
Ubiquitinated. -
細胞内局在
Nucleus. Unassociated with chromosomes during mitosis. - Information by UniProt
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参照データベース
- Entrez Gene: 10951 Human
- Entrez Gene: 12412 Mouse
- Omim: 604511 Human
- SwissProt: P83916 Human
- SwissProt: P83917 Mouse
- Unigene: 77254 Human
- Unigene: 29055 Mouse
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別名
- CBX 1 antibody
- CBX antibody
- Cbx1 antibody
see all
画像
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Lane 1: Wild-type HAP1 cell lysate (40 µg)
Lane 2: CBX1 knockout HAP1 cell lysate (40 µg)
Lane 3: MCF7 cell lysate (40 µg)
Lane 4: A431 cell lysate (40 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab10811 observed at 26 kDa. Red - loading control, ab181602, observed at 37 kDa.ab10811 was shown to specifically react with CBX1 / HP1 beta when CBX1 / HP1 beta knockout samples were used. Wild-type and CBX1 / HP1 beta knockout samples were subjected to SDS-PAGE. Ab10811 and ab181602 (loading control to GAPDH) were diluted at 1/500 and 1/10000 dilution respectively and incubated overnight at 4C. Blots were developed with Goat anti-Rat IgG H&L (IRDye® 800CW) (ab253031) preadsorbed and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777)secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
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Immunocytochemistry/ Immunofluorescence - Anti-CBX1 / HP1 beta antibody [MAC353] - ChIP Grade (ab10811)This image is courtesy of Scott Slattery and Mike ManciniHeLA cells were stained with ab10811 in panel one. In panel two they were stained with ab10811 (green), DAPI (blue) and SH-CREST (red), which stains the centromeres. Fix 30 minutes on ice in 4% formaldehyde in PEM. Quench autofluorescence 2 x 5 minutes with 1 mg/ml Na borohydride or 100 mM ammonium chloride in PEM. Permeablize 30 minutes with 0.5% TX-100 in PEM. Block 30 minutes in 5% milk in TBST. Primary antibody incubated overnight at 4oC diluted 1/100 in 5% milk in TBST. Secondary antibody incubated 1 hour at RT diluted in 5% milk in TBST. Post-fix 20 minutes on ice in 4% formaldehyde in PEM. Quench autofluorescence 2 x 5 minutes with ammonium chloride in PEM. Counterstain with DAPI in TBST. Mount with ProLong Gold antifade reagent from Invitrogen. Notes: Ample washing between each step. TBST = Tris buffered saline + 0.1% Tween. PEM = 80 mM K-PIPES, pH 6.8, 5 mM EGTA, 2 mM MgCl2.
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Immunocytochemistry/ Immunofluorescence - Anti-CBX1 / HP1 beta antibody [MAC353] (ab10811)Image from Talasz H et al., J Biol Chem. 2005 Nov 18;280(46):38814-22. Epub 2005 Sep 15. Fig 1.; doi: 10.1074/jbc.M505563200; November 18, 2005 The Journal of Biological Chemistry, 280, 38814-38822.Immunofluorescence analysis of mouse erythroleukemia cell nuclei, staining CBX1 / HP1 beta in heterochromatin, with ab10811.
Cells were fixed with paraformaldehyde, permeabilized using Triton X-100 and blocked for 30 min with 2.5% BSA. Cells were incubated with primary antibody (1/50) before incubating with a Cy3-conjugated goat anti-rat IgG to detect staining.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (15)
ab10811 は 15 報の論文で使用されています。
- Lamb KN et al. Discovery of Potent Peptidomimetic Antagonists for Heterochromatin Protein 1 Family Proteins. ACS Omega 7:716-732 (2022). PubMed: 35036738
- Qin W et al. Probing protein ubiquitination in live cells. Nucleic Acids Res 50:e125 (2022). PubMed: 36189882
- Waybright JM et al. A Peptidomimetic Ligand Targeting the Chromodomain of MPP8 Reveals HRP2's Association with the HUSH Complex. ACS Chem Biol 16:1721-1736 (2021). PubMed: 34415726
- Otake K et al. CENP-B creates alternative epigenetic chromatin states permissive for CENP-A or heterochromatin assembly. J Cell Sci 133:N/A (2020). PubMed: 32661090
- Yang G et al. The histone H3K9 methyltransferase SUV39H links SIRT1 repression to myocardial infarction. Nat Commun 8:14941 (2017). PubMed: 28361889