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リコンビナントRabMAb

Anti-CBL (phospho Y674) 抗体 [EPR2227] (ab76536)

Submit a review Submit a question References (2)
Western blot - Anti-CBL (phospho Y674) antibody [EPR2227] (ab76536)
  • Flow Cytometry (Intracellular) - Anti-CBL (phospho Y674) antibody [EPR2227] (ab76536)
  • Anti-CBL (phospho Y674) antibody [EPR2227] (ab76536)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2227] to CBL (phospho Y674)
  • Suitable for: Flow Cyt (Intra), WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-CBL (phospho Y674) antibody [EPR2227]
    CBL 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR2227] to CBL (phospho Y674)

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Flow Cyt (Intra), WBmore details
    適用なし: IHC-P or IP

  • 種交差性

    交差種: Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • Jurkat cell lysate treated with pervanadate. Permeabilized Jurkat cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab76536の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000. Predicted molecular weight: 100 kDa.
特記事項
Flow Cyt (Intra)
1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000. Predicted molecular weight: 100 kDa.
追加情報
Is unsuitable for IHC-P or IP.

ターゲット情報

  • 機能

    Participates in signal transduction in hematopoietic cells. Adapter protein that functions as a negative regulator of many signaling pathways that start from receptors at the cell surface. Acts as an E3 ubiquitin-protein ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and then transfers it to substrates promoting their degradation by the proteasome. Recognizes activated receptor tyrosine kinases, including PDGFA, EGF and CSF1, and terminates signaling.

  • パスウェイ

    Protein modification; protein ubiquitination.

  • 関連疾患

    Defects in CBL are the cause of Noonan syndrome-like disorder (NSL) [MIM:613563]. NSL is a syndrome characterized by a phenotype reminiscent of Noonan syndrome. Clinical features are highly variable, including facial dysmorphism, short neck, developmental delay, hyperextensible joints and thorax abnormalities with widely spaced nipples. The facial features consist of triangular face with hypertelorism, large low-set ears, ptosis, and flat nasal bridge. Some patients manifest cardiac defects.

  • 配列類似性

    Contains 1 Cbl-PTB (Cbl-type phosphotyrosine-binding) domain.
    Contains 1 RING-type zinc finger.
    Contains 1 UBA domain.

  • ドメイン

    The RING-type zinc finger domain mediates binding to an E2 ubiquitin-conjugating enzyme.
    The N-terminus is composed of the phosphotyrosine binding (PTB) domain, a short linker region and the RING-type zinc finger. The PTB domain, which is also called TKB (tyrosine kinase binding) domain, is composed of three different subdomains: a four-helix bundle (4H), a calcium-binding EF hand and a divergent SH2 domain.

  • 翻訳後修飾

    Phosphorylated on tyrosine residues by EGFR, SYK, FYN and ZAP70 (By similarity). Phosphorylated on tyrosine residues by INSR.

  • 細胞内局在

    Cytoplasm.
  • Information by UniProt

  • 参照データベース

    • 別名

      • 4732447J05Rik antibody
      • C CBL antibody
      • Cas Br M (murine) ecotropic retroviral transforming sequence antibody
      • Casitas B lineage lymphoma proto oncogene antibody
      • Casitas B-lineage lymphoma proto-oncogene antibody
      • CBL 2 antibody
      • cbl antibody
      • CBL_HUMAN antibody
      • CBL2 antibody
      • E3 ubiquitin protein ligase CBL antibody
      • E3 ubiquitin-protein ligase CBL antibody
      • Oncogene CBL2 antibody
      • Proto oncogene c CBL antibody
      • Proto-oncogene c-CBL antibody
      • RGD1561386 antibody
      • RING finger protein 55 antibody
      • RNF55 antibody
      • Signal transduction protein CBL antibody
      see all

    画像

    • Western blot - Anti-CBL (phospho Y674) antibody [EPR2227] (ab76536)
      Western blot - Anti-CBL (phospho Y674) antibody [EPR2227] (ab76536)
      All lanes : Anti-CBL (phospho Y674) antibody [EPR2227] (ab76536) at 1/1000 dilution

      Lane 1 : Jurkat cell
      lysate
      Lane 2 : Jurkat cell
      lysate treated with
      pervanadate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/1000 dilution

      Predicted band size: 100 kDa
      Observed band size: 120 kDa why is the actual band size different from the predicted?

    • Flow Cytometry (Intracellular) - Anti-CBL (phospho Y674) antibody [EPR2227] (ab76536)
      Flow Cytometry (Intracellular) - Anti-CBL (phospho Y674) antibody [EPR2227] (ab76536)

      Intracellular flow cytometric analysis of permeabilized Jurkat cells, un-treated (green) or pervanadate treated (red) using ab76536 at a 1/20 dilution, and pervanadate-treated Jurkat cells using the same antibody pre-incubated with phospho-CBL peptide (blue) or non-phospho-CBL peptide (orange).

       

    • Anti-CBL (phospho Y674) antibody [EPR2227] (ab76536)
      Anti-CBL (phospho Y674) antibody [EPR2227] (ab76536)

    参考文献 (2)

    ab76536 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab76536 は 2 報の論文で使用されています。

    • Garcia EG  et al. PRL3 enhances T-cell acute lymphoblastic leukemia growth through suppressing T-cell signaling pathways and apoptosis. Leukemia 35:679-690 (2021). PubMed: 32606318
    • Bunaciu RP  et al. Potential for subsets of wt-NPM1 primary AML blasts to respond to retinoic acid treatment. Oncotarget 9:4134-4149 (2018). PubMed: 29423110

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