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リコンビナントRabMAb

Anti-Caveolin-1 (phospho Y14) 抗体 [EPR2288Y] (ab75876)

Submit a review Submit a question References (1)
Western blot - Anti-Caveolin-1 (phospho Y14) antibody [EPR2288Y] (ab75876)
  • Western blot - Anti-Caveolin-1 (phospho Y14) antibody [EPR2288Y] (ab75876)
  • Dot Blot - Anti-Caveolin-1 (phospho Y14) antibody [EPR2288Y] (ab75876)
  • Anti-Caveolin-1 (phospho Y14) antibody [EPR2288Y] (ab75876)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2288Y] to Caveolin-1 (phospho Y14)
  • Suitable for: Dot blot, WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-Caveolin-1 (phospho Y14) antibody [EPR2288Y]
    Caveolin-1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR2288Y] to Caveolin-1 (phospho Y14)

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Dot blot, WBmore details
    適用なし: Flow Cyt,ICC/IF,IHC-P or IP

  • 種交差性

    交差種: Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: A673, Insulin treated A673, A673 treated with insulin then the membrane was incubated with phosphatase, HeLa and HeLa cell treated with pervanadate whole cell lysate.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab75876の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Dot blot
1/1000.
WB
1/2000 - 1/5000. Predicted molecular weight: 20 kDa.
特記事項
Dot blot
1/1000.
WB
1/2000 - 1/5000. Predicted molecular weight: 20 kDa.
追加情報
Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.

ターゲット情報

  • 機能

    May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity (By similarity). Involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Recruits CTNNB1 to caveolar membranes and may regulate CTNNB1-mediated signaling through the Wnt pathway.

  • 組織特異性

    Expressed in muscle and lung, less so in liver, brain and kidney.

  • 関連疾患

    Defects in CAV1 are the cause of congenital generalized lipodystrophy type 3 (CGL3) [MIM:612526]; also called Berardinelli-Seip congenital lipodystrophy type 3 (BSCL3). Congenital generalized lipodystrophies are autosomal recessive disorders characterized by a near absence of adipose tissue, extreme insulin resistance, hypertriglyceridemia, hepatic steatosis and early onset of diabetes.

  • 配列類似性

    Belongs to the caveolin family.

  • 翻訳後修飾

    The initiator methionine for isoform Beta is removed during or just after translation. The new N-terminal amino acid is then N-acetylated.

  • 細胞内局在

    Golgi apparatus membrane. Cell membrane. Membrane > caveola. Membrane raft. Colocalized with DPP4 in membrane rafts. Potential hairpin-like structure in the membrane. Membrane protein of caveolae.
  • Information by UniProt

  • 参照データベース

    • 別名

      • BSCL3 antibody
      • CAV antibody
      • CAV1 antibody
      • CAV1_HUMAN antibody
      • caveolae protein, 22 kD antibody
      • caveolin 1 alpha isoform antibody
      • caveolin 1 beta isoform antibody
      • Caveolin 1 caveolae protein 22kDa antibody
      • Caveolin-1 antibody
      • Caveolin1 antibody
      • cell growth-inhibiting protein 32 antibody
      • CGL3 antibody
      • LCCNS antibody
      • MSTP085 antibody
      • OTTHUMP00000025031 antibody
      • PPH3 antibody
      • VIP 21 antibody
      • VIP21 antibody
      see all

    画像

    • Western blot - Anti-Caveolin-1 (phospho Y14) antibody [EPR2288Y] (ab75876)
      Western blot - Anti-Caveolin-1 (phospho Y14) antibody [EPR2288Y] (ab75876)
      All lanes : Anti-Caveolin-1 (phospho Y14) antibody [EPR2288Y] (ab75876) at 1/1000 dilution

      Lane 1 : Untreated A673 (Human muscle Ewing's Sarcoma) whole cell lysates
      Lane 2 : A673 (Human muscle Ewing's Sarcoma) treated with insulin, whole cell lysates
      Lane 3 : A673 (Human muscle Ewing's Sarcoma) treated with insulin, whole cell lysates. Then the membrane was incubated with phosphatase.

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 20 kDa


      Exposure time: 30 seconds


      Blocking/Diluting buffer 5% NFDM/TBST

    • Western blot - Anti-Caveolin-1 (phospho Y14) antibody [EPR2288Y] (ab75876)
      Western blot - Anti-Caveolin-1 (phospho Y14) antibody [EPR2288Y] (ab75876)
      All lanes : Anti-Caveolin-1 (phospho Y14) antibody [EPR2288Y] (ab75876) at 1/2000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : HeLa cell lysate treated with pervanadate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/1000 dilution

      Predicted band size: 20 kDa
      Observed band size: 22 kDa why is the actual band size different from the predicted?

    • Dot Blot - Anti-Caveolin-1 (phospho Y14) antibody [EPR2288Y] (ab75876)
      Dot Blot - Anti-Caveolin-1 (phospho Y14) antibody [EPR2288Y] (ab75876)

      Dot blot analysis of Caveolin-1 (phospho Y14) phospho peptide (Lane 1), Caveolin-1 Non-phospho peptide (Lane 2) labelling Caveolin-1 (phospho Y14) with ab75876 at a dilution of 1/1000. Peroxidase conjugated goat anti-rabbit IgG (H+L)) was used as the secondary antibody at a dilution of 1/2500.

      Blocking and diluting buffer: 5% NFDM/TBST.

      Exposure time: 3 minutes.

    • Anti-Caveolin-1 (phospho Y14) antibody [EPR2288Y] (ab75876)
      Anti-Caveolin-1 (phospho Y14) antibody [EPR2288Y] (ab75876)

    参考文献 (1)

    ab75876 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab75876 は 1 報の論文で使用されています。

    • Zhao D  et al. CXCR4 promotes gefitinib resistance of Huh7 cells by activating the c-Met signaling pathway. FEBS Open Bio 11:3115-3125 (2021). PubMed: 34555268

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