Anti-Caveolin-1 抗体 (ab36152)
Key features and details
- Goat polyclonal to Caveolin-1
- Suitable for: Flow Cyt (Intra), ICC, WB
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Caveolin-1 antibody
Caveolin-1 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to Caveolin-1 -
由来種
Goat -
特異性
No cross-reactivity expected to Caveolin-2 and Caveolin-3. -
アプリケーション
適用あり: Flow Cyt (Intra), ICC, WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Dog, Pig
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免疫原
Synthetic peptide corresponding to Human Caveolin-1 aa 33-44 (internal sequence).
Sequence:DELSEKQVYDAH
Database link: Q03135 -
ポジティブ･コントロール
- ICC: U-2 OS and A431 cells; WB: A549 and A431 cell lysates. Human heart tissue lysate. Flow Cyt (Intra): HeLa cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituent: 0.5% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab36152の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt (Intra) |
Use a concentration of 10 µg/ml.
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| ICC |
Use a concentration of 10 µg/ml.
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| WB | (3) |
Use a concentration of 0.001 - 0.01 µg/ml. Detects a band of approximately 21, 25 kDa (predicted molecular weight: 20 kDa).
1 hour primary incubation is recommended for this product. |
| 特記事項 |
|---|
|
Flow Cyt (Intra)
Use a concentration of 10 µg/ml. |
|
ICC
Use a concentration of 10 µg/ml. |
|
WB
Use a concentration of 0.001 - 0.01 µg/ml. Detects a band of approximately 21, 25 kDa (predicted molecular weight: 20 kDa). 1 hour primary incubation is recommended for this product. |
ターゲット情報
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機能
May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity (By similarity). Involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Recruits CTNNB1 to caveolar membranes and may regulate CTNNB1-mediated signaling through the Wnt pathway. -
組織特異性
Expressed in muscle and lung, less so in liver, brain and kidney. -
関連疾患
Defects in CAV1 are the cause of congenital generalized lipodystrophy type 3 (CGL3) [MIM:612526]; also called Berardinelli-Seip congenital lipodystrophy type 3 (BSCL3). Congenital generalized lipodystrophies are autosomal recessive disorders characterized by a near absence of adipose tissue, extreme insulin resistance, hypertriglyceridemia, hepatic steatosis and early onset of diabetes. -
配列類似性
Belongs to the caveolin family. -
翻訳後修飾
The initiator methionine for isoform Beta is removed during or just after translation. The new N-terminal amino acid is then N-acetylated. -
細胞内局在
Golgi apparatus membrane. Cell membrane. Membrane > caveola. Membrane raft. Colocalized with DPP4 in membrane rafts. Potential hairpin-like structure in the membrane. Membrane protein of caveolae. - Information by UniProt
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参照データベース
- Entrez Gene: 403980 Dog
- Entrez Gene: 857 Human
- Entrez Gene: 12389 Mouse
- Entrez Gene: 404693 Pig
- Entrez Gene: 25404 Rat
- Omim: 601047 Human
- SwissProt: P33724 Dog
- SwissProt: Q03135 Human
see all -
別名
- BSCL3 antibody
- CAV antibody
- CAV1 antibody
see all
画像
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Immunocytochemistry - Anti-Caveolin-1 antibody (ab36152)
Immunocytochemistry analysis of paraformaldehyde fixed A431 (human epidermoid carcinoma cell line) cells labeling Caveolin-1 with ab36152 at 10 µg/mL. Cells permeabilized with 0.15% Triton. Primary incubation for 1 hour followed by Alexa Fluor® 488 secondary antibody at 2 µg/mL (green). The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor® 488 secondary antibody at 2 µg/mL.
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Western blot - Anti-Caveolin-1 antibody (ab36152)Anti-Caveolin-1 antibody (ab36152) at 0.003 µg/ml + Human Heart lysate at 35 µg
Predicted band size: 20 kDa -
Flow Cytometry (Intracellular) - Anti-Caveolin-1 antibody (ab36152)Flow Cytometry (Intracellular) analysis of paraformaldehyde fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Caveolin-1 with ab36152 at 10 µg/mL (blue line). Cells permeabilized with 0.5% Triton. Primary incubation overnight followed by Alexa Fluor® 488 secondary antibody at 1 µg/mL.
IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor® 488 secondary antibody.
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Western blot - Anti-Caveolin-1 antibody (ab36152)Lane 1 : Anti-Caveolin-1 antibody (ab36152) at 0.003 µg/ml
Lane 2 : Anti-Caveolin-1 antibody (ab36152) at 0.001 µg/ml
Lane 1 : A431 cell lysate
Lane 2 : A549 cell lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 20 kDa -
Immunocytochemistry - Anti-Caveolin-1 antibody (ab36152)Immunocytochemistry analysis of paraformaldehyde fixed U-2 OS (human bone osteosarcoma epithelial cell line) cells labeling Caveolin-1 with ab36152 at 10 µg/mL. Cells permeabilized with 0.15% Triton. Primary incubation for 1 hour followed by Alexa Fluor® 488 secondary antibody at 2 µg/mL (green). The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor® 488 secondary antibody at 2 µg/mL.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (9)
ab36152 は 9 報の論文で使用されています。
- Peixoto-Neves D et al. KV7.1 channel blockade inhibits neonatal renal autoregulation triggered by a step decrease in arterial pressure. Am J Physiol Renal Physiol 322:F197-F207 (2022). PubMed: 35001664
- Defourny J et al. Efnb2 haploinsufficiency induces early gap junction plaque disassembly and endocytosis in the cochlea. Brain Res Bull 174:153-160 (2021). PubMed: 34139316
- Scicchitano M et al. Icariin Protects H9c2 Rat Cardiomyoblasts from Doxorubicin-Induced Cardiotoxicity: Role of Caveolin-1 Upregulation and Enhanced Autophagic Response. Nutrients 13:N/A (2021). PubMed: 34836326
- Takagaki Y et al. Dipeptidyl peptidase-4 plays a pathogenic role in BSA-induced kidney injury in diabetic mice. Sci Rep 9:7519 (2019). PubMed: 31101909
- Lenis TL et al. Expression of ABCA4 in the retinal pigment epithelium and its implications for Stargardt macular degeneration. Proc Natl Acad Sci U S A 115:E11120-E11127 (2018). PubMed: 30397118
- Stucki JD et al. Medium throughput breathing human primary cell alveolus-on-chip model. Sci Rep 8:14359 (2018). PubMed: 30254327
- Cuddapah VA et al. Calcium entry via TRPC1 channels activates chloride currents in human glioma cells. Cell Calcium 53:187-94 (2013). PubMed: 23261316
- Hunt CL et al. The Tyro3 receptor kinase Axl enhances macropinocytosis of Zaire ebolavirus. J Virol 85:334-47 (2011). WB ; Human . PubMed: 21047970
- Schmidtko A et al. cGMP produced by NO-sensitive guanylyl cyclase essentially contributes to inflammatory and neuropathic pain by using targets different from cGMP-dependent protein kinase I. J Neurosci 28:8568-76 (2008). IHC-FoFr ; Mouse . PubMed: 18716216
