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AB251056

Anti-Cathepsin W 抗体 [EPR15135] - BSA and Azide free

Anti-Cathepsin W antibody [EPR15135] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal Cathepsin W antibody. Carrier free. Suitable for IP, WB and reacts with Human, Rat samples. Cited in 1 publication.

別名を表示する

Cathepsin W, Lymphopain, CTSW

4 Images
Immunoprecipitation - Anti-Cathepsin W antibody [EPR15135] - BSA and Azide free (AB251056)
  • IP

Supplier Data

Immunoprecipitation - Anti-Cathepsin W antibody [EPR15135] - BSA and Azide free (AB251056)

This data was developed using ab191083, the same antibody clone in a different buffer formulation.

Western blot analysis of immunoprecipitation pellet from K562 cell lysate immunoprecipitated using ab191083 at 1/30 dilution (lane 1) or PBS control (lane 2).
Secondary : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

All lanes:

Immunoprecipitation - Anti-Cathepsin W antibody [EPR15135] (<a href='/products/primary-antibodies/cathepsin-w-antibody-epr15135-ab191083'>ab191083</a>)

Predicted band size: 42 kDa

false

Western blot - Anti-Cathepsin W antibody [EPR15135] - BSA and Azide free (AB251056)
  • WB

Supplier Data

Western blot - Anti-Cathepsin W antibody [EPR15135] - BSA and Azide free (AB251056)

This data was developed using ab191083, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Cathepsin W antibody [EPR15135] (<a href='/products/primary-antibodies/cathepsin-w-antibody-epr15135-ab191083'>ab191083</a>) at 1/20000 dilution

Lane 1:

A431 cell lysate at 10 µg

Lane 2:

SH-SY5Y cell lysate at 10 µg

Lane 3:

K562 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugate at 1/1000 dilution

Predicted band size: 42 kDa

false

Western blot - Anti-Cathepsin W antibody [EPR15135] - BSA and Azide free (AB251056)
  • WB

Supplier Data

Western blot - Anti-Cathepsin W antibody [EPR15135] - BSA and Azide free (AB251056)

This data was developed using ab191083, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Cathepsin W antibody [EPR15135] (<a href='/products/primary-antibodies/cathepsin-w-antibody-epr15135-ab191083'>ab191083</a>) at 1/50000 dilution

Lane 1:

HuT-78 cell lysate at 20 µg

Lane 2:

MOLT-4 cell lysate at 20 µg

Lane 3:

Jurkat cell lysate at 20 µg

Lane 4:

HeLa cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 42 kDa

false

Western blot - Anti-Cathepsin W antibody [EPR15135] - BSA and Azide free (AB251056)
  • WB

Supplier Data

Western blot - Anti-Cathepsin W antibody [EPR15135] - BSA and Azide free (AB251056)

This data was developed using ab191083, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Cathepsin W antibody [EPR15135] (<a href='/products/primary-antibodies/cathepsin-w-antibody-epr15135-ab191083'>ab191083</a>) at 1/10000 dilution

All lanes:

PC12 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugate at 1/1000 dilution

Predicted band size: 42 kDa

false

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR15135

アイソタイプ

IgG

キャリアフリー

Yes

交差種

Rat, Human

アプリケーション

WB, IP

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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製品の詳細

ab251056 is the carrier-free version of ab191083.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.2 - 7.4 Constituents: PBS
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
+4°C
保管に関する情報
Do Not Freeze
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Cathepsin W also known as CTSW is a member of the cysteine protease family. It has a molecular mass of approximately 45 kDa and functions as an enzyme that breaks down protein substrates through proteolysis. CTSW displays specificity for certain peptide bonds in its target proteins. This protein is mostly expressed in the immune system especially in cytotoxic T cells and natural killer cells. The expression pattern suggests its involvement in immune responses.
Biological function summary

Cathepsin W contributes to the modulation of cytotoxic activity. It assists in the rearrangement and processing of proteins important for the immune response facilitating the proper functioning of immune effector cells. While not part of a specific protein complex CTSW activity aligns with immune cell regulation mechanisms. Its role in the degradation of proteins plays a significant part in maintaining immune cell competency.

Pathways

Cathepsin W contributes to the immune response and apoptosis. The immune response pathway involves cytotoxic T cells and natural killer cells where CTSW enhances the degradation of target proteins. In apoptosis the protein functions in the breakdown of cellular components that need recycling or removal. Cathepsin W shares functional similarities with related proteases such as Cathepsin C which also participates in immune cell regulation and apoptosis.

Cathepsin W has relevance in immunological diseases and some cancer types. Altered activity of CTSW may associate with impaired immune responses contributing to immunodeficiency conditions. In certain cancers such as leukemia changes in CTSW expression could influence the behavior of tumor-infiltrating lymphocytes. Proteins like granzyme B which are involved in inducing apoptosis might interact with CTSW reflecting their shared pathways in modulating immune responses and disease progression.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

May have a specific function in the mechanism or regulation of T-cell cytolytic activity.. (Microbial infection) Plays a role during influenza virus infection in lungs cells ex vivo. Acts at the level of virus entering host cytoplasm from late endosome.
See full target information CTSW
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine reports 21:454-462 PubMed31746378

2019

Blueberry anthocyanin‑enriched extract ameliorates transverse aortic constriction‑induced myocardial dysfunction via the DDAH1/ADMA/NO signaling pathway in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Weiqing Hu,Wenyue Wang,Qing Ma,Tao Liu,Jiefeng Zhang,Jicun Zhang
View all publications
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