Anti-Cathepsin V 抗体 [EPR10723(B)] (ab166894)
![Western blot - Anti-Cathepsin V antibody [EPR10723(B)] (ab166894)](https://www.abcam.co.jp/ps/products/166/ab166894/Images/ab166894-165238-anti-cathepsin-v-antibody-epr10723b-western-blot.jpg "Western blot - Anti-Cathepsin V antibody [EPR10723(B)] (ab166894)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10723(B)] to Cathepsin V
- Suitable for: Flow Cyt (Intra), WB, ICC/IF, IP
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Cathepsin V antibody [EPR10723(B)]
Cathepsin V 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR10723(B)] to Cathepsin V -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra), WB, ICC/IF, IPmore details
適用なし: IHC-P -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- A549, HeLa, SW480, fetal kidney and Human small intestine lysates; A549 cells; HeLa cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
EPR10723(B) -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab166894の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 37 kDa.
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| ICC/IF |
1/250 - 1/500.
|
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| IP |
1/10 - 1/100.
|
| 特記事項 |
|---|
|
Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/1000 - 1/10000. Predicted molecular weight: 37 kDa. |
|
ICC/IF
1/250 - 1/500. |
|
IP
1/10 - 1/100. |
追加情報
Is unsuitable for IHC-P.
ターゲット情報
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機能
Cysteine protease. May have an important role in corneal physiology. -
組織特異性
Predominantly expressed in the thymus and testis. Also expressed in corneal epithelium, and to a lesser extent in conjunctival epithelium and skin. -
配列類似性
Belongs to the peptidase C1 family. -
細胞内局在
Lysosome. - Information by UniProt
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参照データベース
- Entrez Gene: 1515 Human
- Omim: 603308 Human
- SwissProt: O60911 Human
- Unigene: 610096 Human
- Unigene: 621851 Human
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別名
- CAT L2 antibody
- Cathepsin L2 antibody
- Cathepsin U antibody
see all
画像
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Western blot - Anti-Cathepsin V antibody [EPR10723(B)] (ab166894)All lanes : Anti-Cathepsin V antibody [EPR10723(B)] (ab166894) at 1/1000 dilution
Lane 1 : A549 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : SW480 cell lysate
Lane 4 : Fetal kidney tissue lysate
Lane 5 : Human small intestine tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 37 kDa -
![Immunoprecipitation - Anti-Cathepsin V antibody [EPR10723(B)] (ab166894)](https://www.abcam.co.jp/ps/products/166/ab166894/Images/ab166894-165237-anti-cathepsin-v-antibody-epr10723b-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Cathepsin V antibody [EPR10723(B)] (ab166894)Western blot analysis on immunoprecipitation pellet from Human small intestine lysate, using ab166894 at 1/1000 and HRP-conjugated anti-rabbit IgG, preferentially detecting the non-reduced form of rabbit IgG. -
![Immunocytochemistry/ Immunofluorescence - Anti-Cathepsin V antibody [EPR10723(B)] (ab166894)](https://www.abcam.co.jp/ps/products/166/ab166894/Images/ab166894-165236-anti-cathepsin-v-antibody-epr10723b-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Cathepsin V antibody [EPR10723(B)] (ab166894)Immunofluorescent analysis of A549 cells labeling Cathepsin V with ab166894 at 1/250 dilution. -
![Flow Cytometry (Intracellular) - Anti-Cathepsin V antibody [EPR10723(B)] (ab166894)](https://www.abcam.co.jp/ps/products/166/ab166894/Images/ab166894-165235-anti-cathepsin-v-antibody-epr10723b-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-Cathepsin V antibody [EPR10723(B)] (ab166894)Intracellular flow cytometric analysis of permeabilized HeLa using ab166894 at 1/10 dilution (red) or a rabbit IgG (negative) (green).
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![Anti-Cathepsin V antibody [EPR10723(B)] (ab166894)](https://www.abcam.co.jp/ps/products/166/ab166894/Images/ab166894-5-benefits-of-recombinant-antibodies.png)
Anti-Cathepsin V antibody [EPR10723(B)] (ab166894)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab166894 は 2 報の論文で使用されています。
- Yang L et al. Glycosylated Cathepsin V Serves as a Prognostic Marker in Lung Cancer. Front Oncol 12:876245 (2022). PubMed: 35494076
- Yi WJ et al. Degraded melanocores are incompetent to protect epidermal keratinocytes against UV damage. Cell Cycle 17:844-857 (2018). PubMed: 29623762