Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
リコンビナントRabMAb

Anti-Cathepsin K 抗体 [EPR24829-101] (BSA and Azide free) (ab300570)

Submit a review Submit a question
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin K antibody [EPR24829-101] (BSA and Azide free) (AB300570)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin K antibody [EPR24829-101] (BSA and Azide free) (AB300570)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin K antibody [EPR24829-101] (BSA and Azide free) (AB300570)
  • Immunohistochemistry (Frozen sections) - Anti-Cathepsin K antibody [EPR24829-101] (BSA and Azide free) (AB300570)
  • Immunohistochemistry (Frozen sections) - Anti-Cathepsin K antibody [EPR24829-101] (BSA and Azide free) (AB300570)
  • Anti-Cathepsin K antibody [EPR24829-101] (BSA and Azide free) (AB300570)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR24829-101] to Cathepsin K - BSA and Azide free
  • Suitable for: IHC-P, IHC-Fr
  • Reacts with: Mouse, Rat

Conjugates logo Related conjugates and formulations

Unconjugated

製品の概要

  • 製品名

    Anti-Cathepsin K antibody [EPR24829-101] (BSA and Azide free)
    Cathepsin K 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR24829-101] to Cathepsin K - BSA and Azide free

  • 由来種

    Rabbit

  • 特異性

    We do not recommend this antibody for mouse IHC-Fr.

  • アプリケーション

    適用あり: IHC-P, IHC-Frmore details
    適用なし: Flow Cyt (Intra),ICC/IF,IP or WB

  • 種交差性

    交差種: Mouse, Rat
    非交差種: Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • IHC-P: Mouse bone, mouse kidney, rat bone, FFPE tissue sections. IHCFr: Rat P0 bone, aat liver fresh frozen tissues.

  • 特記事項

    ab300570 is the carrier-free version of ab300569.

     

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab300570の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IHC-Fr
Use at an assay dependent concentration.
特記事項
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IHC-Fr
Use at an assay dependent concentration.
追加情報
Is unsuitable for Flow Cyt (Intra),ICC/IF,IP or WB.

ターゲット情報

  • 機能

    Closely involved in osteoclastic bone resorption and may participate partially in the disorder of bone remodeling. Displays potent endoprotease activity against fibrinogen at acid pH. May play an important role in extracellular matrix degradation.

  • 組織特異性

    Predominantly expressed in osteclasts (bones).

  • 関連疾患

    Defects in CTSK are the cause of pycnodysostosis (PKND) [MIM:265800]. PKND is an autosomal recessive osteochondrodysplasia characterized by osteosclerosis and short stature.

  • 配列類似性

    Belongs to the peptidase C1 family.

  • 細胞内局在

    Lysosome.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Cathepsin K antibody
      • Cathepsin O antibody
      • Cathepsin O1 antibody
      • Cathepsin O2 antibody
      • Cathepsin X antibody
      • CATK_HUMAN antibody
      • CTS02 antibody
      • Ctsk antibody
      • CTSO antibody
      • CTSO1 antibody
      • CTSO2 antibody
      • MGC23107 antibody
      • PKND antibody
      • PYCD antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin K antibody [EPR24829-101] (BSA and Azide free) (AB300570)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin K antibody [EPR24829-101] (BSA and Azide free) (AB300570)

      This data was developed using ab300569, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling Cathepsin K with ab300569 at 1/1000 dilution (0.529 µg/mL) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection kit). Negative control: no staining on mouse kidney (PMID:8647859). The section was incubated with ab300569 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: PBS was used instead of primary antibody, followed by a ready to use secondary antibody LeicaDS9800 (Bond™ Polymer Refine Detection kit).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin K antibody [EPR24829-101] (BSA and Azide free) (AB300570)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin K antibody [EPR24829-101] (BSA and Azide free) (AB300570)

      This data was developed using ab300569, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded rat bone tissue labeling Cathepsin K with ab300569 at 1/1000 dilution (0.529 µg/mL) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection kit). Positive staining on osteoclasts in rat bone (PMID:29322325). The section was incubated with ab300569 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: PBS was used instead of primary antibody, followed by a ready to use secondary antibody LeicaDS9800 (Bond™ Polymer Refine Detection kit).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin K antibody [EPR24829-101] (BSA and Azide free) (AB300570)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin K antibody [EPR24829-101] (BSA and Azide free) (AB300570)

      This data was developed using ab300569, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded mouse bone tissue labeling Cathepsin K with ab300569 at 1/1000 dilution (0.529 µg/mL) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection kit). Positive staining on osteoclasts in mouse bone (PMID:29322325). The section was incubated with ab300569 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: PBS was used instead of primary antibody, followed by a ready to use secondary antibody LeicaDS9800 (Bond™ Polymer Refine Detection kit).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    • Immunohistochemistry (Frozen sections) - Anti-Cathepsin K antibody [EPR24829-101] (BSA and Azide free) (AB300570)
      Immunohistochemistry (Frozen sections) - Anti-Cathepsin K antibody [EPR24829-101] (BSA and Azide free) (AB300570)

      This data was developed using ab300569, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat liver (fresh) tissue labeling Cathepsin K with ab300569 at 1/100 (5.29 µg/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 µg/ml) dilution (Green). Negative control: no staining on rat liver (PMID:25356585). is observed. The nuclear counterstain was DAPI (Blue).

      Secondary antibody control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 (2 µg/ml) dilution.

    • Immunohistochemistry (Frozen sections) - Anti-Cathepsin K antibody [EPR24829-101] (BSA and Azide free) (AB300570)
      Immunohistochemistry (Frozen sections) - Anti-Cathepsin K antibody [EPR24829-101] (BSA and Azide free) (AB300570)

      This data was developed using ab300569, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat P0 bone (fresh) tissue labeling Cathepsin K with ab300569 at 1/100 (5.29 µg/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 µg/ml) dilution (Green). Positive staining on osteoclasts of rat P0 bone is observed. The nuclear counterstain was DAPI (Blue).

      Secondary antibody control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 (2 µg/ml) dilution.

    • Anti-Cathepsin K antibody [EPR24829-101] (BSA and Azide free) (AB300570)
      Anti-Cathepsin K antibody [EPR24829-101] (BSA and Azide free) (AB300570)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab300570 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab300570 は論文での使用が確認できていません。

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    There are currently no Customer reviews or Questions for ab300570.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com