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リコンビナントRabMAb

Anti-Cathepsin K 抗体 [EPR24829-101] (ab300569)

Submit a review Submit a question References (2)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin K antibody [EPR24829-101] (AB300569)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin K antibody [EPR24829-101] (AB300569)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin K antibody [EPR24829-101] (AB300569)
  • Immunohistochemistry (Frozen sections) - Anti-Cathepsin K antibody [EPR24829-101] (AB300569)
  • Immunohistochemistry (Frozen sections) - Anti-Cathepsin K antibody [EPR24829-101] (AB300569)
  • Anti-Cathepsin K antibody [EPR24829-101] (AB300569)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR24829-101] to Cathepsin K
  • Suitable for: IHC-Fr, IHC-P
  • Reacts with: Mouse, Rat

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-Cathepsin K antibody [EPR24829-101]
    Cathepsin K 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR24829-101] to Cathepsin K

  • 由来種

    Rabbit

  • 特異性

    We do not recommend this antibody for mouse IHC-Fr.

  • アプリケーション

    適用あり: IHC-Fr, IHC-Pmore details
    適用なし: Flow Cyt (Intra),ICC/IF,IP or WB

  • 種交差性

    交差種: Mouse, Rat
    非交差種: Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • IHC-P: Mouse bone, rat bone, FFPE tissue sections. IHC-Fr: Rat P0 bone.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab300569の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-Fr
1/100.
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
特記事項
IHC-Fr
1/100.
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
追加情報
Is unsuitable for Flow Cyt (Intra),ICC/IF,IP or WB.

ターゲット情報

  • 機能

    Closely involved in osteoclastic bone resorption and may participate partially in the disorder of bone remodeling. Displays potent endoprotease activity against fibrinogen at acid pH. May play an important role in extracellular matrix degradation.

  • 組織特異性

    Predominantly expressed in osteclasts (bones).

  • 関連疾患

    Defects in CTSK are the cause of pycnodysostosis (PKND) [MIM:265800]. PKND is an autosomal recessive osteochondrodysplasia characterized by osteosclerosis and short stature.

  • 配列類似性

    Belongs to the peptidase C1 family.

  • 細胞内局在

    Lysosome.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Cathepsin K antibody
      • Cathepsin O antibody
      • Cathepsin O1 antibody
      • Cathepsin O2 antibody
      • Cathepsin X antibody
      • CATK_HUMAN antibody
      • CTS02 antibody
      • Ctsk antibody
      • CTSO antibody
      • CTSO1 antibody
      • CTSO2 antibody
      • MGC23107 antibody
      • PKND antibody
      • PYCD antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin K antibody [EPR24829-101] (AB300569)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin K antibody [EPR24829-101] (AB300569)

      Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling Cathepsin K with ab300569 at 1/1000 dilution (0.529 µg/mL) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection kit). Negative control: no staining on mouse kidney (PMID:8647859). The section was incubated with ab300569 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: PBS was used instead of primary antibody, followed by a ready to use secondary antibody LeicaDS9800 (Bond™ Polymer Refine Detection kit).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin K antibody [EPR24829-101] (AB300569)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin K antibody [EPR24829-101] (AB300569)

      Immunohistochemical analysis of paraffin-embedded rat bone tissue labeling Cathepsin K with ab300569 at 1/1000 dilution (0.529 µg/mL) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection kit). Positive staining on osteoclasts in rat bone (PMID:29322325). The section was incubated with ab300569 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: PBS was used instead of primary antibody, followed by a ready to use secondary antibody LeicaDS9800 (Bond™ Polymer Refine Detection kit).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin K antibody [EPR24829-101] (AB300569)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin K antibody [EPR24829-101] (AB300569)

      Immunohistochemical analysis of paraffin-embedded mouse bone tissue labeling Cathepsin K with ab300569 at 1/1000 dilution (0.529 µg/mL) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection kit). Positive staining on osteoclasts in mouse bone (PMID:29322325). The section was incubated with ab300569 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: PBS was used instead of primary antibody, followed by a ready to use secondary antibody LeicaDS9800 (Bond™ Polymer Refine Detection kit).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    • Immunohistochemistry (Frozen sections) - Anti-Cathepsin K antibody [EPR24829-101] (AB300569)
      Immunohistochemistry (Frozen sections) - Anti-Cathepsin K antibody [EPR24829-101] (AB300569)

      Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat liver (fresh) tissue labeling Cathepsin K with ab300569 at 1/100 (5.29 µg/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 µg/ml) dilution (Green). Negative control: no staining on rat liver (PMID:25356585). is observed. The nuclear counterstain was DAPI (Blue).

      Secondary antibody control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 (2 µg/ml) dilution.

    • Immunohistochemistry (Frozen sections) - Anti-Cathepsin K antibody [EPR24829-101] (AB300569)
      Immunohistochemistry (Frozen sections) - Anti-Cathepsin K antibody [EPR24829-101] (AB300569)

      Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat P0 bone (fresh) tissue labeling Cathepsin K with ab300569 at 1/100 (5.29 µg/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 µg/ml) dilution (Green). Positive staining on osteoclasts of rat P0 bone is observed. The nuclear counterstain was DAPI (Blue).

      Secondary antibody control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 (2 µg/ml) dilution.

    • Anti-Cathepsin K antibody [EPR24829-101] (AB300569)
      Anti-Cathepsin K antibody [EPR24829-101] (AB300569)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (2)

    ab300569 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab300569 は 2 報の論文で使用されています。

    • Meng B  et al. Dual Role of Interleukin-20 in Different Stages of Osteoclast Differentiation and Its Osteoimmune Regulation during Alveolar Bone Remodeling. Int J Mol Sci 24:N/A (2023). PubMed: 36835229
    • Zhao MN  et al. A novel microRNA-182/Interleukin-8 regulatory axis controls osteolytic bone metastasis of lung cancer. Cell Death Dis 14:298 (2023). PubMed: 37127752

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