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リコンビナントRabMAb

Anti-Cathepsin K 抗体 [EP9403] (ab187647)

Submit a review Submit a question References (19)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin K antibody [EP9403] (ab187647)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin K antibody [EP9403] (ab187647)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin K antibody [EP9403] (ab187647)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin K antibody [EP9403] (ab187647)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin K antibody [EP9403] (ab187647)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin K antibody [EP9403] (ab187647)
  • Western blot - Anti-Cathepsin K antibody [EP9403] (ab187647)
  • Western blot - Anti-Cathepsin K antibody [EP9403] (ab187647)
  • Western blot - Anti-Cathepsin K antibody [EP9403] (ab187647)
  • Western blot - Anti-Cathepsin K antibody [EP9403] (ab187647)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP9403] to Cathepsin K
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-Cathepsin K antibody [EP9403]
    Cathepsin K 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EP9403] to Cathepsin K

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-Pmore details

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Human and rat cartilage lysate, mouse and rat muscle lysates, human skeletal muscle lysate IHC-P: Human bone, Mouse bone, Rat bone

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab187647の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000 - 1/10000. Detects a band of approximately 39 kDa (predicted molecular weight: 37 kDa).
IHC-P
1/1000.
特記事項
WB
1/1000 - 1/10000. Detects a band of approximately 39 kDa (predicted molecular weight: 37 kDa).
IHC-P
1/1000.

ターゲット情報

  • 機能

    Closely involved in osteoclastic bone resorption and may participate partially in the disorder of bone remodeling. Displays potent endoprotease activity against fibrinogen at acid pH. May play an important role in extracellular matrix degradation.

  • 組織特異性

    Predominantly expressed in osteclasts (bones).

  • 関連疾患

    Defects in CTSK are the cause of pycnodysostosis (PKND) [MIM:265800]. PKND is an autosomal recessive osteochondrodysplasia characterized by osteosclerosis and short stature.

  • 配列類似性

    Belongs to the peptidase C1 family.

  • 細胞内局在

    Lysosome.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • Cathepsin K antibody
    • Cathepsin O antibody
    • Cathepsin O1 antibody
    • Cathepsin O2 antibody
    • Cathepsin X antibody
    • CATK_HUMAN antibody
    • CTS02 antibody
    • Ctsk antibody
    • CTSO antibody
    • CTSO1 antibody
    • CTSO2 antibody
    • MGC23107 antibody
    • PKND antibody
    • PYCD antibody
    see all

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin K antibody [EP9403] (ab187647)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin K antibody [EP9403] (ab187647)

    Immunohistochemical analysis of paraffin-embedded human bone labelling Cathepsin K with ab187647 at 1/1000 (1.307 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

     

    Positive staining on human bone.

     

    The section was incubated with ab187647 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND RX instrument.

     

    Counterstained with Hematoxylin.

     

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

     

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin K antibody [EP9403] (ab187647)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin K antibody [EP9403] (ab187647)

    Immunohistochemical analysis of paraffin-embedded mouse bone labelling Cathepsin K with ab187647 at 1/1000 (1.307 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

     

    Positive staining on mouse bone.

     

    The section was incubated with ab187647 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND RX instrument.

     

    Counterstained with Hematoxylin.

     

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

     

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin K antibody [EP9403] (ab187647)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin K antibody [EP9403] (ab187647)

    Immunohistochemical analysis of paraffin-embedded rat bone labelling Cathepsin K with ab187647 at 1/1000 (1.307 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

     

    Positive staining on rat bone.

     

    The section was incubated with ab187647 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND RX instrument.

     

    Counterstained with Hematoxylin.

     

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

     

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin K antibody [EP9403] (ab187647)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin K antibody [EP9403] (ab187647)

    Immunohistochemical analysis of paraffin-embedded rat liver with ab187647 at 1/1000 (1.037 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Negative control: No staining on rat liver.

    The section was incubated with ab187647 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND RX instrument.

    Counterstained with Hematoxylin.

    Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin K antibody [EP9403] (ab187647)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin K antibody [EP9403] (ab187647)

    Immunohistochemical analysis of paraffin-embedded mouse liver with ab187647 at 1/1000 (1.037 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Negative control: No staining on mouse liver.

    The section was incubated with ab187647 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND RX instrument.

    Counterstained with Hematoxylin.

    Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin K antibody [EP9403] (ab187647)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin K antibody [EP9403] (ab187647)

    Immunohistochemical analysis of paraffin-embedded Human liver with ab187647 at 1/1000 (1.037 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Negative control: No staining on human liver.

    The section was incubated with ab187647 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND RX instrument.

    Counterstained with Hematoxylin.

    Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.

  • Western blot - Anti-Cathepsin K antibody [EP9403] (ab187647)
    Western blot - Anti-Cathepsin K antibody [EP9403] (ab187647)
    All lanes : Anti-Cathepsin K antibody [EP9403] (ab187647) at 1/1000 dilution (purified)

    Lane 1 : Mouse cartilage lysates
    Lane 2 : Rat muscle lysates
    Lane 3 : Mouse muscle lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 37 kDa
    Observed band size: 39 kDa why is the actual band size different from the predicted?

  • Western blot - Anti-Cathepsin K antibody [EP9403] (ab187647)
    Western blot - Anti-Cathepsin K antibody [EP9403] (ab187647)
    All lanes : Anti-Cathepsin K antibody [EP9403] (ab187647) at 1/1000 dilution (Purified)

    Lane 1 : Human cartilage lysates
    Lane 2 : Human skeletal muscle lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 37 kDa
    Observed band size: 39 kDa why is the actual band size different from the predicted?

  • Western blot - Anti-Cathepsin K antibody [EP9403] (ab187647)
    Western blot - Anti-Cathepsin K antibody [EP9403] (ab187647)
    Anti-Cathepsin K antibody [EP9403] (ab187647) at 1/5000 dilution (unpurified) + Rat cartilage lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 37 kDa
    Additional bands at: 39 kDa. We are unsure as to the identity of these extra bands.

  • Western blot - Anti-Cathepsin K antibody [EP9403] (ab187647)
    Western blot - Anti-Cathepsin K antibody [EP9403] (ab187647)
    Anti-Cathepsin K antibody [EP9403] (ab187647) at 1/1000 dilution (unpurified) + Mouse muscle lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 37 kDa
    Additional bands at: 39 kDa. We are unsure as to the identity of these extra bands.

参考文献 (19)

ab187647 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab187647 は 19 報の論文で使用されています。

  • Liu K  et al. Atsttrin regulates osteoblastogenesis and osteoclastogenesis through the TNFR pathway. Commun Biol 6:1251 (2023). PubMed: 38081906
  • Lv H  et al. A static magnetic field improves bone quality and balances the function of bone cells with regulation on iron metabolism and redox status in type 1 diabetes. FASEB J 37:e22985 (2023). PubMed: 37249350
  • Toejing P  et al. Accelerated Bone Loss in Transgenic Mice Expressing Constitutively Active TGF-β Receptor Type I. Int J Mol Sci 24:N/A (2023). PubMed: 37445982
  • Wang X  et al. Melatonin induces RAW264.7 cell apoptosis via the BMAL1/ROS/MAPK-p38 pathway to improve postmenopausal osteoporosis. Bone Joint Res 12:677-690 (2023). PubMed: 37907083
  • Liu J  et al. WTAP-Mediated m6A RNA Methylation Regulates the Differentiation of Bone Marrow Mesenchymal Stem Cells via the miR-29b-3p/HDAC4 Axis. Stem Cells Transl Med 12:307-321 (2023). PubMed: 37010483
View all Publications for this product

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