Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online

Anti-Cathepsin K 抗体 [3F9] (ab37259)

Submit a review Q&A (2)References (37)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin K antibody [3F9] (ab37259)
  • Western blot - Anti-Cathepsin K antibody [3F9] (ab37259)
  • Flow Cytometry - Anti-Cathepsin K antibody [3F9] (ab37259)

Key features and details

  • Mouse monoclonal [3F9] to Cathepsin K
  • Suitable for: WB, IHC-P, Flow Cyt
  • Reacts with: Mouse, Human
  • Isotype: IgG2b

製品の概要

  • 製品名

    Anti-Cathepsin K antibody [3F9]
    Cathepsin K 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [3F9] to Cathepsin K

  • 由来種

    Mouse

  • アプリケーション

    適用あり: WB, IHC-P, Flow Cytmore details

  • 種交差性

    交差種: Mouse, Human

  • 免疫原

    Recombinant fragment (6x HIS-tag) corresponding to Human Cathepsin K aa 100-350.

  • エピトープ

    The monoclonal antibody ab37259 (clone 3F9) reacts with an epitope located between aa 115-329: APDSVDYRKKGYVTPNQGQCGSCWAFSSVGALEGQLKKKTGKLLNLSPQNLVDCVSENDGC GGGYMTNAFQYVQKNRGIDSEDAYPYVGQEESCMYNPTGKAAKCRGYREIPEGNEKALKRA VARVGPVSVAIDASLTSFQFSKGVYYDESCNSDNLNHAVLAVGYGIQKGNKHWIIKNSWGE NWGNKGYILMARNKNNACGIANLASFPKM

  • 特記事項

    This product was changed from ascites to tissue culture supernatant on 22nd December 2017. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab37259の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 37, 40 kDa (predicted molecular weight: 37 kDa).
IHC-P
Use at an assay dependent concentration.
Flow Cyt
Use 1µg for 106 cells.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. 
特記事項
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 37, 40 kDa (predicted molecular weight: 37 kDa).
IHC-P
Use at an assay dependent concentration.
Flow Cyt
Use 1µg for 106 cells.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. 

ターゲット情報

  • 機能

    Closely involved in osteoclastic bone resorption and may participate partially in the disorder of bone remodeling. Displays potent endoprotease activity against fibrinogen at acid pH. May play an important role in extracellular matrix degradation.

  • 組織特異性

    Predominantly expressed in osteclasts (bones).

  • 関連疾患

    Defects in CTSK are the cause of pycnodysostosis (PKND) [MIM:265800]. PKND is an autosomal recessive osteochondrodysplasia characterized by osteosclerosis and short stature.

  • 配列類似性

    Belongs to the peptidase C1 family.

  • 細胞内局在

    Lysosome.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Cathepsin K antibody
      • Cathepsin O antibody
      • Cathepsin O1 antibody
      • Cathepsin O2 antibody
      • Cathepsin X antibody
      • CATK_HUMAN antibody
      • CTS02 antibody
      • Ctsk antibody
      • CTSO antibody
      • CTSO1 antibody
      • CTSO2 antibody
      • MGC23107 antibody
      • PKND antibody
      • PYCD antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin K antibody [3F9] (ab37259)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin K antibody [3F9] (ab37259)
      Bone from mouse foot (bone marrow with osteoclasts (positive). IHC-P image was obtained after deparaffinization, antigen retrieval with 0.1 M EDTA (6-8 min, 72 C), drying of slide before staining (10 min, 62 C), and subsequent staining with primary antibody (1/100) in 1% horse serum (1 h, 37 C).
    • Western blot - Anti-Cathepsin K antibody [3F9] (ab37259)
      Western blot - Anti-Cathepsin K antibody [3F9] (ab37259)
      Anti-Cathepsin K antibody [3F9] (ab37259) at 1 µg/ml + Human bone tumor tissue lysate - total protein (ab29359) at 10 µg

      Secondary
      Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed at 1/3000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 37 kDa
      Observed band size: 37,40 kDa why is the actual band size different from the predicted?
      Additional bands at: 26 kDa (possible cleavage fragment)


      Exposure time: 1 minute


      Cathepsin K contains a potential glycosylation site (SwissProt) which may explain its migration at a higher molecular weight than predicted, seen at 40 kDa. The band observed at 25 kDa could potentially be a cleaved form of Cathepsin K due to the presence of both a 15 amino acid signal prptide and a 99 amino acid propeptide. Cathepsin K in its mature form has an expected molecular weight of 25 kDa.
    • Flow Cytometry - Anti-Cathepsin K antibody [3F9] (ab37259)
      Flow Cytometry - Anti-Cathepsin K antibody [3F9] (ab37259)
      Overlay histogram showing U20S cells stained with ab37259 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab37259, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in U20S cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

    参考文献 (37)

    ab37259 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab37259 は 37 報の論文で使用されています。

    • Ohnishi T  et al. Loss of function mutation in Ank causes aberrant mineralization and acquisition of osteoblast-like-phenotype by the cells of the intervertebral disc. Cell Death Dis 14:447 (2023). PubMed: 37468461
    • Sviercz FA  et al. Massively HIV-1-infected macrophages exhibit a severely hampered ability to differentiate into osteoclasts. Front Immunol 14:1206099 (2023). PubMed: 37404829
    • Liu Y  et al. Exploration of clinicopathological features of rearranged renal cell carcinoma and TFE3, TFEB, and ALK staining performance in renal entities. Heliyon 9:e15159 (2023). PubMed: 37089387
    • Du J  et al. BRD9-mediated chromatin remodeling suppresses osteoclastogenesis through negative feedback mechanism. Nat Commun 14:1413 (2023). PubMed: 36918560
    • Giannikou K  et al. TSC2 inactivation, low mutation burden and high macrophage infiltration characterise hepatic angiomyolipomas. Histopathology 83:569-581 (2023). PubMed: 37679051
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-2 of 2 Abreviews or Q&A

    Question

    Buongiorno,

    vi scrivo in italiano e spero riusciate a tradurre.

    Sto cercando di mettere a punto l’Ab Cathepsin K (ab37259) con il sistema di rivelazione Dako FLEX+. Dal data sheet si evince che il seguente metodo:

    1) antigen retrieval : EDTA 6-8’ a 72°C
    2) passaggio successivo: asciugare le sezioni 10’ a 62°C
    3) incubazione Ab primario ( diluito 1:100 in 1% di siero normale di cavallo ) per 1h a 37°C


    domande:

    1.Perchè l’antigen retrieval solo a 72°C anziché i canonici 98°C?
    2.Perché asciugare le sezioni, dato che è una certezza da sempre non dover mai asciugare le sezioni?
    3.Perché diluire l’Ab primario in horse serum 1% e perché incubarlo a 37°C? E’ forse molto debole? Infatti la diluizione consigliata prevede una concentrazione a 10µg/ml… abbastanza alta


    Resto in attesa di chiarimenti

    Grazie per la collaborazione

    Read More
    Answer

    Grazie per la sua domanda. Vi rispondo in Inglese - se ha bisogno di una traduzione, mi faccia sapere per favore. Grazie!
    Thank you for your inquiry.
    The protocol on the datasheet is this protocol is from a customer who tested our antibody and provided his results and photo. The information about the drying is from him too. Unfortunately we do not know more.
    As the tissue he used was bone as well, I would suspect that on bone tissue you can have special protocols (albeit decalcification is before fixation normally).
    In any case, I can recommend you to follow your protocol which is established in your laboratory. I would recommend a normal heat induced antigen retrieval with boiling and only if your tissue is very sensitive, to do antigen retrieval overnight at 70°C. I would also recommend to test different antigen retrieval solutions at different pHs, as we recommend for all our antibodies.
    I do also absolutely agree with you, that after rehydratation the sections should never dry out again, as this will impair the staining strongly.
    The antibody incubation can be done at 37°C, however we recommend to incubate the antibody either 1 to 2 hours at room temperature or overnight at 4°C. This will be absolutely sufficient.
    So in summary, I can recommend you to perform the immunostaining with this antibody as you would do normally. We do guarantee this antibody to work also with a classical IHC protocol.
    I hope this information is helpful. Please do not hesitate to contact us again should you have any further question.

    Read More

    Question

    Dear Colleagues

    We have a customer that asks us, referred to ab37259, why you recommend the following steps: “Bone from mouse foot (bone marrow with osteoclasts (positive). IHC-P image was obtained after deparaffinization, antigen retrieval with 0.1 M EDTA (6-8 min, 72 C), drying of slide before staining (10 min, 62 C), and subsequent staining with primary antibody (1/100) in 1% horse serum (1 h, 37 C)”

    Thanks a lot for your precious collaboration

    With my best regards

    Read More
    Answer

    Thank you for contacting us and your interest in our products.

    I am sorry if the product datasheet has been misleading for your customer. The details provided with the image are to illustrate how this particular IHC staining experiment was performed. They are not intended as a recommendation of how IHC experiments have to be performed. For example the following reference have not used this method when staining human cancer tissue:

    Birgersdotter A et al. Inflammation and tissue repair markers distinguish the nodular sclerosis and mixed cellularity subtypes of classical Hodgkin's lymphoma. Br J Cancer 101:1393-401 (2009). PubMed: 19773754

    The information is provided as a guideline only and optimization is likely to be required depending on the tissue and protocol used by the customer. More information on how to optimize IHC experiments can be found from the following link:

    https://www.abcam.com/index.html?pageconfig=resource&rid=11384

    I hope this information has been of help. If you require any further information please do not hesitate to contact us again.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com