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AB36161

Anti-Cathepsin F 抗体

Anti-Cathepsin F antibody

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(1 Publication)

Goat Polyclonal Cathepsin F antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human CTSF aa 450 to C-terminus.

別名を表示する

Cathepsin F, CATSF, CTSF

1 Images
Western blot - Anti-Cathepsin F antibody (AB36161)
  • WB

Unknown

Western blot - Anti-Cathepsin F antibody (AB36161)

Primary incubation was 1 hour. Detected by chemiluminescence. Experiments gave bands at approx 30kDa and 35kDa in Human Prostate and Ovary lysates after 0.3µg/ml antibody staining. These bands correspond to earlier findings in literature (Wang et al, J Biol Chem. 1998 Nov 27;273(48) : 32000-8; PMID : 9822672). This protein has a calculated MW of 53.4kDa according to NP_003784.2.

All lanes:

Western blot - Anti-Cathepsin F antibody (ab36161) at 0.3 µg/mL

All lanes:

Human Ovary lysate (35µg protein in RIPA buffer).

Predicted band size: 53 kDa

false

Key facts

宿主種

Goat

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

No

交差種

Human

アプリケーション

WB

applications

免疫原

Synthetic Peptide within Human CTSF aa 450 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9UBX1

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.3-1 µg/mL", "WB-species-notes": "<p>1 hour primary incubation is recommended for this product.</p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Immunogen
精製に関する特記事項
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
バッファー組成
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 0.5% BSA
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Cathepsin F also known as CTSF is a cysteine protease enzyme with a molecular mass of approximately 44 kDa. It belongs to the papain-like protease family and exhibits proteolytic activity. This protein localizes to the lysosomes where it participates in protein degradation. Cathepsin F shows expression in various tissues including liver kidney and spleen suggesting a broad role in protein catabolism.
Biological function summary

Cathepsin F functions in protein breakdown processes within the lysosomal compartment. It actively participates in the formation and maturation of the autophagosomes. This protease is not known to be a component of a multi-protein complex but it works in concert with other lysosomal enzymes to ensure efficient catabolic activity. Its activity helps in the turnover of intracellular proteins maintaining cellular homeostasis.

Pathways

Cathepsin F contributes significantly to the lysosomal degradation pathway. It assists in proteolytic cascades that help in breaking down proteins into amino acids. This degradation pathway is important for the recycling of cellular components and energy production. Moreover Cathepsin F shares functional relationships with other proteases like Cathepsin B and Cathepsin L as they often have overlapping substrate specificities and contribute to a similar biological pathway.

Cathepsin F has links to neurodegenerative diseases such as Alzheimer's disease and certain types of cancer. In Alzheimer's disease Cathepsin F's altered activity affects protein clearance associated with amyloid beta aggregation. This protease also relates to Cancer Progression through its role in matrix remodeling. In neurodegenerative conditions and cancer Cathepsin F's interaction with proteins like Cathepsin S has implications for disease progression. Understanding these connections highlights Cathepsin F’s importance in diagnosing and potentially targeting therapeutic interventions.

製品プロトコール

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ターゲットの情報

Thiol protease which is believed to participate in intracellular degradation and turnover of proteins. Has also been implicated in tumor invasion and metastasis.
See full target information CTSF

文献 (1)

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Biochimica et biophysica acta 1833:2254-66 PubMed23684953

2013

N-terminally truncated forms of human cathepsin F accumulate in aggresome-like inclusions.

Applications

Unspecified application

Species

Human

Barbara Jerič,Iztok Dolenc,Marko Mihelič,Martina Klarić,Tina Zavašnik-Bergant,Gregor Gunčar,Boris Turk,Vito Turk,Veronika Stoka
View all publications

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