AB125067

Anti-Cathepsin B 抗体 [EPR4323]

Anti-Cathepsin B antibody [EPR4323]

RabMAb
Recombinant
20ul selling size
KO Validated
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(29 Publications)

Rabbit Recombinant Monoclonal Cathepsin B antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 29 publications.

別名を表示する

CPSB, CTSB, Cathepsin B, APP secretase, Cathepsin B1, APPS

5 Images

Immunocytochemistry/ Immunofluorescence - Anti-Cathepsin B antibody [EPR4323] (AB125067)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Cathepsin B antibody [EPR4323] (AB125067)

ab125067, at a 1/50 dilution, staining Cathepsin B (red) in HeLa cells by immunofluorescence. DNA is marked in blue.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin B antibody [EPR4323] (AB125067)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin B antibody [EPR4323] (AB125067)

ab125067, at a 1/50 dilution, staining Cathepsin B in paraffin-embedded Human papillary carcinoma tissue by immunohistochemistry.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin B antibody [EPR4323] (AB125067)

ab125067, at a 1/50 dilution, staining Cathepsin B in paraffin-embedded Human kidney tissue by immunohistochemistry.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Collaborator

Western blot - Anti-Cathepsin B antibody [EPR4323] (AB125067)

ab125067 was shown to react with CTSB in wild-type HAP1 cells in Western blot with loss of signal observed in a CTSB knockout cell line. Wild-type HAP1 and CTSB knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with ab125067 overnight at 4 °C at a 1/1000 dilution. Blots were incubated with secondary antibodies at 0.2 µg/mL before imaging.

This data was provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.

All lanes:

Western blot - Anti-Cathepsin B antibody [EPR4323] (ab125067) at 1/1000 dilution

Lane 1:

Wild-type HAP1 lysate at 30 µg

Lane 2:

CTSB knock-out HAP1 lysate at 30 µg

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Unknown

Western blot - Anti-Cathepsin B antibody [EPR4323] (AB125067)

All lanes:

Western blot - Anti-Cathepsin B antibody [EPR4323] (ab125067) at 1/1000 dilution

Lane 1:

HT1080 cell lysate at 10 µg

Lane 2:

A375 cell lysate at 10 µg

Lane 3:

SW480 cell lysate at 10 µg

Lane 4:

A549 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-Rabbit HRP at 1/2000 dilution

Predicted band size: 38 kDa

true

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR4323

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

IHC-P, ICC/IF, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特異性

Our in‑house QC data shows only the pro–cathepsin B band, with no additional cleaved chains detected.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "" } } }

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製品の詳細

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

-20°C

保管に関する情報

Stable for 12 months at -20°C

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Cathepsin B also known as CTSB or cathepsin beta is a lysosomal cysteine protease belonging to the papain family. This enzyme possesses a molecular weight of approximately 25 to 30 kDa. You can find cathepsin B expressed in various tissues with high levels observed in the liver kidney and spleen. It plays a mechanical role in protein degradation within lysosomes operating optimally in acidic environments. The enzyme has active and inactive forms depending on structural modifications and environmental conditions.

Biological function summary

Cathepsin B participates as a proteolytic enzyme involved in protein turnover and processing. It does not function in isolation but often associates with various other proteases forming complexes essential for efficient substrate breakdown. Cathepsin B assists in the activation of other enzymes by cleaving propeptides therefore facilitating the initiation of cascade reactions. This role is critical in maintaining cellular homeostasis and modulating physiological processes including cell migration and immune response modulation.

Pathways

Cathepsin B significantly contributes to the lysosomal protein degradation pathway and the autophagy-lysosome pathway. It interacts with proteins such as B protein which are important for cellular recycling and energy homeostasis. Its activity supports the MHC class II antigen processing pathway playing an essential part in immune system functionality. Cathepsin B also exhibits interactions with Ctsb protein inhibitors which regulate its proteolytic activity within these pathways.

Cathepsin B has strong associations with cancer progression and neurological disorders such as Alzheimer's disease. Its overexpression and dysregulation are linked to tumor metastasis where it degrades extracellular matrix components promoting invasion. Ctsb protein interactions with corey mazo proteins influence tumor microenvironment and immune evasion. In Alzheimer's disease cathepsin B can influence amyloid beta peptide processing connected to neurodegenerative pathology progression. These roles highlight the potential for cathepsin B as a therapeutic target in addressing these conditions.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Thiol protease which is believed to participate in intracellular degradation and turnover of proteins (PubMed : 12220505). Cleaves matrix extracellular phosphoglycoprotein MEPE (PubMed : 12220505). Involved in the solubilization of cross-linked TG/thyroglobulin in the thyroid follicle lumen (By similarity). Has also been implicated in tumor invasion and metastasis (PubMed : 3972105).

See full target information CTSB

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文献 (29)

Recent publications for all applications. Explore the full list and refine your search

Biology direct 20:100 PubMed41035046

2025

MINPP1 promotes ferroptosis in HBV-related hepatocellular carcinoma by regulating CTSB K33-linked deubiquitination via ZRANB1.

Applications

Unspecified application

Species

Unspecified reactive species

Wenbiao Chen,Yang Song,Liyang Wang,Feng Xiong,Shenggang Zhang

European journal of medical research 30:403 PubMed40394663

2025

Inhibiting cathepsin B alleviates acute lung injury caused by sepsis through suppression of pyroptosis in lung epithelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaobo Zhang,Zhuojun Deng,Xinyu Zhang,Qian Xu,Li Liu,Dong Yang,Zimeng Guo

Molecular imaging and biology 26:1057-1067 PubMed39331316

2024

Immunohistochemical Evaluation of Cathepsin B, L, and S Expression in Breast Cancer Patients.

Applications

Unspecified application

Species

Unspecified reactive species

Daan G J Linders,Okker D Bijlstra,Laura C Fallert,N Geeske Dekker-Ensink,Taryn L March,Martin Pool,Ethan Walker,Brian Straight,James P Basilion,Matthew Bogyo,Jacobus Burggraaf,Denise E Hilling,Alexander L Vahrmeijer,Peter J K Kuppen,A Stijn L P Crobach

npj aging 10:24 PubMed38704365

2024

EPB41L4A-AS1 is required to maintain basal autophagy to modulates Aβ clearance.

Applications

Unspecified application

Species

Unspecified reactive species

Ziqiang Wang,Ruomei Wang,Lixin Niu,Xiaoyan Zhou,Jinxiang Han,Kun Li

Cancer research communications 4:970-985 PubMed38517140

2024

Versican Associates with Tumor Immune Phenotype and Limits T-cell Trafficking via Chondroitin Sulfate.

Applications

Unspecified application

Species

Unspecified reactive species

Priyanka Hirani,Jacqueline McDermott,Vinothini Rajeeve,Pedro R Cutillas,J Louise Jones,Daniel J Pennington,Thomas N Wight,Salvatore Santamaria,Kimberly M Alonge,Oliver M T Pearce

Journal of orthopaedic surgery and research 18:877 PubMed37980493

2023

Circ_0020014 mediates CTSB expression and participates in IL-1β-prompted chondrocyte injury via interacting with miR-24-3p.

Applications

Unspecified application

Species

Unspecified reactive species

Chenpeng Zhang,Wenjun He

Chemical biology & drug design 102:1409-1420 PubMed37599208

2023

Hederagenin inhibits high glucose-induced fibrosis in human renal cells by suppression of NLRP3 inflammasome activation through reducing cathepsin B expression.

Applications

Unspecified application

Species

Unspecified reactive species

Guohua Yang,Wang Yang,Hairong Jiang,Qing Yi,Wei Ma

Molecular cell 83:2524-2539.e7 PubMed37390818

2023

Lysosomal LAMP proteins regulate lysosomal pH by direct inhibition of the TMEM175 channel.

Applications

Unspecified application

Species

Unspecified reactive species

Jiyuan Zhang,Weizhong Zeng,Yan Han,Wan-Ru Lee,Jen Liou,Youxing Jiang

Cell 185:2292-2308.e20 PubMed35750034

2022

Parkinson's disease-risk protein TMEM175 is a proton-activated proton channel in lysosomes.

Applications

Unspecified application

Species

Unspecified reactive species

Meiqin Hu,Ping Li,Ce Wang,Xinghua Feng,Qi Geng,Wei Chen,Matangi Marthi,Wenlong Zhang,Chenlang Gao,Whitney Reid,Joel Swanson,Wanlu Du,Richard I Hume,Haoxing Xu

Cancers 14: PubMed35626132

2022

diTFPP, a Phenoxyphenol, Sensitizes Hepatocellular Carcinoma Cells to C-Ceramide-Induced Autophagic Stress by Increasing Oxidative Stress and ER Stress Accompanied by LAMP2 Hypoglycosylation.

Applications

Unspecified application

Species

Unspecified reactive species

Chien-Chih Chiu,Yen-Chun Chen,Yung-Ding Bow,Jeff Yi-Fu Chen,Wangta Liu,Jau-Ling Huang,En-De Shu,Yen-Ni Teng,Chang-Yi Wu,Wen-Tsan Chang

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Cathepsin B 抗体 [EPR4323]

Anti-Cathepsin B antibody [EPR4323]

Immunocytochemistry/ Immunofluorescence - Anti-Cathepsin B antibody [EPR4323] (AB125067)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin B antibody [EPR4323] (AB125067)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin B antibody [EPR4323] (AB125067)

Western blot - Anti-Cathepsin B antibody [EPR4323] (AB125067)

Western blot - Anti-Cathepsin B antibody [EPR4323] (AB125067)

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Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

特異性

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存温度

長期保存温度

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (29)

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Abcam product promise