Anti-Cathepsin B 抗体 [CA10] (ab58802)

The immunogen used to create ab58802 is purified cathepsin B from human liver. The epitope has been mapped to the heavy chain of mature cathepsin B, but no more specifics are known.

We have no data showing whether or not this antibody will cross react with the other cathepsins. That being said, the monoclonal antibody has a very long and trusted publication history:
http://scholar.google.com/scholar?hl=en&q=ca10+cathepsin&btnG=&as_sdt=1%2C22&as_sdtp=


If you would like to try this antibody in rabbit, you could participate in our AbTrial program to test it risk free. Please contact me if you're interested: www.abcam.com/abtrial

Thank you for your updates.

I am very sorry to hear that the customer is having problem with this antibody as well. It could well be that the product has been damaged during shipment to India. Currently, we have a few alternative antibodies for Cathepsin B; please advise your customer to take a look at our catalogue. I could certainly offer you an alternative product as a replacement.

I look forward to hearing from you soon.

I am sorry to hear that you have been experiencing problems using this product in the application that you wish.

In order to assess the quality of our products I would ask that you complete a brief questionnaire relating to the application used. Often it is possible to make suggestions that may help resolve problems experienced using a particular product.

As our Abpromise indicates, in the event that a product is not functioning in the applications/species cited on the product data sheet (and the problem has been reported within 6 months of purchase) we will happily offer a credit note/refund to the value of the product purchased.

All our customer feedback, including complaints are monitored weekly by our in house technical support team. If a product is at fault the technical support team will consider removing the product from our catalogue in order to avoid future customer inconvenience.

Could you provide some further details of the protocol used and complete the following form (attached as a word document). It would be much appreciated if you could attach an image to the response.

Thank you for your understanding and co-operation in this matter. I look forward to hearing from you soon and resolving this issue as soon as possible.

Thank you for contacting us.

Unfortunately, alignment of our immunogen with mosquito Cathepsin B shows identity below threshold for reactivity of this antibody in this species. An immunogen should have 85% identity with the target of interest. I was able to repeat this alignment with each of our Cathepsin B antibodies and did not find one which we could recommend for use with mosquito. As such these products would not be eligible for our AbTrial program.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

I am very pleased to hear you would like to test ab5076, ab8049, ab18258, ab58802, ab64581, ab82259 and send us your results. The testing discount doesn't apply to ab4674 since this has already been tested in Rhesus monkey and IHC so we would guarantee that experiment to work.

The codes will give you a value off your next orders before the expiration date. To redeem this offer, please submit 6 Abreview with rhesus monkey data and include the code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

The code will be active once the Abreview wit has been submitted and can be redeemed in one of the following ways:
1) Call to place your order and mention the code to our customer service department;
2) Include the code in your fax order;
3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated. Remember if the product does not work you will be covered by our Abpromise guarantee and eligible for a full refund or replacement.

If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: https://www.abcam.com/abtrial.

Thank you for taking time toanswer my questions.

I would like to offer some suggestions to help optimize the results:

1. First thing first: ab77355 is a goat antibody. The secondary antibody will cross reacts with Bovine Ig present in BSA due to a high homology. Therefore, use the serum of the secondary as a blocking agentif possible. http://www.immunoportal.com/modules.php?name=gallery2&g2_itemId=9222 This should help a lot to lower the background.

2. In order to avoid over-loading and therefore to decrease the side bands, I would like to suggest loading 20 to 30 ug protein per lane.

3. As the blocking agent is a protein itself, prolonged blocking and in a high concentration may lead to some unwanted adherence to the blot. A maximum of 2h with 5% BSA should be enough and avoid this phenomena.

4. To clear up the image it is advisable to filter the blocking solution prior to incubation.

5. Please decrease the concentration of ab77355 down to 1:5000 to increase the specificity and to lower the background.

6. Please be aware that both antibodies needs to be stored at -20C. ab58802: Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Store at 4°C until reconstituted, then store in aliquots at -20°C or at 4°C with 0.1% azide.

7. Please perform an incubation with the secondary antibody only to check if the secondary reacts with the sample.


Should the suggestions not improve the results, please do let me know.

In the event that a product is not functioning in the species and applications cited on the product datasheet (and the problem has been reported within 6 months of purchase), we would be pleased to provide a free of charge replacement, credit note, or refund.

I hope this information is helpful, and I thank you for your cooperation.

Thank you for contacting us.

Your credit note ID is ******.

I am sorry that this antibody did not perform as stated on the datasheet, I have asked our Finance department to issue a credit note for you. The credit note may be used in one of the following ways:

(1) Redeemed against the original invoice if this hasn't already been paid.
(2) Held on the account for use against a future order.

Please contact your Finance department to confirm how you would like the credit note to be used and ensure it is not redeemed without your knowledge.

The credit note ID is for your reference only, please refer to the credit note ID in any correspondence with our accounting department. We will send you the completed credit note by email or postal mail with the actual credit note number which will start with the letters CGB.

I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service should you require further expert advice

Thank you for taking time to check these additional details. The details provided will enable us to investigate this case. Itam very sorrythatthe results with this antibodyhave not been successful.

I have checked the information provided and compared the two antibodies. Indeed, as the ab58802 is a monoclonal antibody, it should provide not more bands than the polyclonal antibody. It appears thereforethat you may have received a faulty vial.

I apologize for the inconvenience and am pleased to offer you a free of charge replacement or acredit note.

Thank you for your cooperation. I look forward to hearing from you with details of how you and the customerwould like to proceed.