Anti-Cathepsin B 抗体 (ab30443)
Key features and details
- Rabbit polyclonal to Cathepsin B
- Suitable for: ELISA, IP, ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Cathepsin B antibody
Cathepsin B 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Cathepsin B -
由来種
Rabbit -
アプリケーション
適用あり: ELISA, IP, ICC/IF, WBmore details -
種交差性
交差種: Human -
免疫原
Full length native protein (purified): Cathepsin B from Human liver.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Add glycerol to a final volume of 50% for extra stability and aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
IgG fraction -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab30443の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
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| IP |
Use at an assay dependent concentration. PubMed: 24833013
|
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| ICC/IF | (1) |
Use at an assay dependent concentration.
See Abreview. |
| WB |
Use at an assay dependent concentration.
|
| 特記事項 |
|---|
|
ELISA
Use at an assay dependent concentration. |
|
IP
Use at an assay dependent concentration. PubMed: 24833013 |
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ICC/IF
Use at an assay dependent concentration. See Abreview. |
|
WB
Use at an assay dependent concentration. |
ターゲット情報
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機能
Thiol protease which is believed to participate in intracellular degradation and turnover of proteins. Has also been implicated in tumor invasion and metastasis. -
配列類似性
Belongs to the peptidase C1 family. -
細胞内局在
Lysosome. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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参照データベース
- Entrez Gene: 1508 Human
- Omim: 116810 Human
- SwissProt: P07858 Human
- Unigene: 520898 Human
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別名
- Amyloid precursor protein secretase antibody
- APP secretase antibody
- APPS antibody
see all
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab30443 は 5 報の論文で使用されています。
- Chien WW et al. Unexpected cross-reactivity of anti-cathepsin B antibodies leads to uncertainties regarding the mechanism of action of anti-CD20 monoclonal antibody GA101. Leuk Res 55:41-48 (2017). PubMed: 28122282
- Han X et al. AMPK activation protects cells from oxidative stress-induced senescence via autophagic flux restoration and intracellular NAD(+) elevation. Aging Cell 15:416-27 (2016). PubMed: 26890602
- Lohoefer F et al. Quantitative expression and localization of cysteine and aspartic proteases in human abdominal aortic aneurysms. Exp Mol Med 46:e95 (2014). WB, IP ; Human . PubMed: 24833013
- Li C et al. Expression and clinical significance of cathepsin B and stefin A in laryngeal cancer. Oncol Rep 26:869-75 (2011). PubMed: 21667036
- Krohne TU et al. Lipid peroxidation products reduce lysosomal protease activities in human retinal pigment epithelial cells via two different mechanisms of action. Exp Eye Res 90:261-6 (2010). PubMed: 19895809