Anti-Catalase 抗体 (ab50434)
Key features and details
- Goat polyclonal to Catalase
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Catalase antibody
Catalase 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to Catalase -
由来種
Goat -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Guinea pig, Cow, Dog, Common marmoset, Orangutan
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免疫原
Synthetic peptide:
C-DKYNAEKPKNAIHT
, corresponding to internal sequence amino acids 498-511 of Human Catalase -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab50434の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).
1 hour primary incubation is recommended for this product. |
| IHC-P |
Use a concentration of 2 - 4 µg/ml.
|
| 特記事項 |
|---|
|
WB
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa). 1 hour primary incubation is recommended for this product. |
|
IHC-P
Use a concentration of 2 - 4 µg/ml. |
ターゲット情報
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機能
Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells. -
関連疾患
Defects in CAT are the cause of acatalasia (ACATLAS) [MIM:115500]; also known as acatalasemia. This disease is characterized by absence of catalase activity in red cells and is often associated with ulcerating oral lesions. -
配列類似性
Belongs to the catalase family. -
翻訳後修飾
The N-terminus is blocked. -
細胞内局在
Peroxisome. - Information by UniProt
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参照データベース
- Entrez Gene: 531682 Cow
- Entrez Gene: 403474 Dog
- Entrez Gene: 847 Human
- Entrez Gene: 12359 Mouse
- Omim: 115500 Human
- SwissProt: P00432 Cow
- SwissProt: O97492 Dog
- SwissProt: P04040 Human
see all -
別名
- Cas1 antibody
- CAT antibody
- CATA_HUMAN antibody
see all
画像
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Western blot - Anti-Catalase antibody (ab50434)Anti-Catalase antibody (ab50434) at 1 µg/ml + Human Kidney lysate (35µg protein in RIPA buffer).
Predicted band size: 60 kDa
Observed band size: 60 kDa
Primary incubation was 1 hour. Detected by chemiluminescence.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (4)
ab50434 は 4 報の論文で使用されています。
- Biyik I et al. The effects of recombinant klotho in cisplatin-induced ovarian failure in mice. J Obstet Gynaecol Res 47:1817-1824 (2021). PubMed: 33611838
- Block A et al. The GABAAa5-selective Modulator, RO4938581, Rescues Protein Anomalies in the Ts65Dn Mouse Model of Down Syndrome. Neuroscience 372:192-212 (2018). PubMed: 29292072
- Xie W et al. Exercise training-enhanced, endothelium-dependent dilation mediated by altered regulation of BK(Ca) channels in collateral-dependent porcine coronary arterioles. Microcirculation 20:170-82 (2013). PubMed: 23002811
- Habib LK et al. Inhibitors of Catalase-Amyloid Interactions Protect Cells from {beta}-Amyloid-Induced Oxidative Stress and Toxicity. J Biol Chem 285:38933-43 (2010). IP ; Human . PubMed: 20923778
