Anti-CAST 抗体 (ab76933)
Key features and details
- Rabbit polyclonal to CAST
- Suitable for: WB, IP, ICC, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-CAST antibody
CAST 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to CAST -
由来種
Rabbit -
アプリケーション
適用あり: WB, IP, ICC, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Chimpanzee, Gorilla
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免疫原
Synthetic peptide corresponding to Human CAST aa 400-500. NP_036231.1
Database link: O15446-1 -
ポジティブ･コントロール
- WB: HeLa whole cell lysate. IP: HeLa whole cell lysate; IHC: human breast carcinoma; ICC: HeLa cells
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab76933 was affinity purified using an epitope specific to CAST immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab76933の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/2000 - 1/10000. Predicted molecular weight: 55 kDa.
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| IP |
Use at 2-5 µg/mg of lysate.
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| ICC |
1/50 - 1/2000.
Formaldehyde fixation is recommended. Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells |
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| IHC-P |
1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| 特記事項 |
|---|
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WB
1/2000 - 1/10000. Predicted molecular weight: 55 kDa. |
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IP
Use at 2-5 µg/mg of lysate. |
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ICC
1/50 - 1/2000. Formaldehyde fixation is recommended. Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells |
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IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ターゲット情報
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機能
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors. Isoform 1 is involved in UBTF-activated transcription, presumably at a step following PIC formation.
Isoform 2 has been described as a component of preformed T-cell receptor (TCR) complex. -
配列類似性
Belongs to the eukaryotic RPA34 RNA polymerase subunit family. -
翻訳後修飾
Isoform 2 undergoes tyrosine phosphorylation upon T-cell receptor (TCR) stimulation. This phosphorylation has not been confirmed by other group.
Isoform 1 is phosphorylated on tyrosine residues in initiation-competent Pol I-beta complexes but not in Pol I-alpha complexes.
Phosphorylated upon DNA damage, probably by ATM or ATR. -
細胞内局在
Nucleus > nucleolus. Chromosome. Found at the fibrillar centers of the nucleolus in interphase and during cell division it is localized to the nucleolus organizer regions of the chromosomes. - Information by UniProt
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参照データベース
- Entrez Gene: 10849 Human
- Omim: 107325 Human
- SwissProt: O15446 Human
- Unigene: 710495 Human
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別名
- A34.5 antibody
- Anti sense to ERCC 1 protein antibody
- Antisense to ERCC-1 protein antibody
see all
画像
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Immunocytochemistry - Anti-CAST antibody (ab76933)
Immunocytochemical analysis of formaldehyde-fixed asynchronous HeLa cells aataining CAST with ab76933 at 1/100 dilution and detected by Red-fluorescent goat anti-rabbit IgG highly cross-adsorbed antibody at 1/100 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAST antibody (ab76933)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling CAST with ab76933 at 1/200 (1µg/ml). Detection: DAB.
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Western blot - Anti-CAST antibody (ab76933)All lanes : Anti-CAST antibody (ab76933) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Predicted band size: 55 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
Additional bands at: 130 kDa. We are unsure as to the identity of these extra bands. -
Immunoprecipitation - Anti-CAST antibody (ab76933)ab76933 at 1µg/ml staining CAST in HeLa whole cell lysate immunoprecipitated using ab76933 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab76933 は論文での使用が確認できていません。