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Cell Biology Apoptosis Intracellular Caspases etc Caspases
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Anti-Caspase-8 抗体 (ab25901)

  • Datasheet
  • SDS
Reviews (3)Q&A (2)References (151)

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Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Immunocytochemistry/ Immunofluorescence - Anti-Caspase-8 antibody (ab25901)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-8 antibody (ab25901)
  • Immunocytochemistry/ Immunofluorescence - Anti-Caspase-8 antibody (ab25901)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-8 antibody (ab25901)

Key features and details

  • Rabbit polyclonal to Caspase-8
  • Suitable for: ICC, IHC-P, ICC/IF, WB
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

リコンビナント抗体で、ロット間での高い再現性を実現

Product image
Anti-Pro Caspase-8 antibody [EPR162] (ab108333)
  • 異なるロット間での安定した再現性
  • 容易なスケールアップ
  • 評価試験による特異性の確認済み
  • 倫理基準に準拠 - アニマル・フリーの生産

製品の概要

  • 製品名

    Anti-Caspase-8 antibody
    Caspase-8 一次抗体 製品一覧
  • 製品の詳細

    Rabbit polyclonal to Caspase-8
  • 由来種

    Rabbit
  • アプリケーション

    適用あり: ICC, IHC-P, ICC/IF, WBmore details
  • 種交差性

    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to Human Caspase-8 (C terminal). Synthetic peptide (Human) - a 16 amino acid peptide from near the carboxy-terminus of human Caspase-8 isoform A.
    Database link: Q14790

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at +4°C.
  • バッファー

    pH: 7.2
    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • 精製度

    Immunogen affinity purified
  • 特記事項(精製)

    Caspase-8 Antibody is affinity chromatography purified via peptide column.
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

    • Cell Biology
    • Apoptosis
    • Intracellular
    • Caspases etc
    • Caspases
    • Cancer
    • Invasion/microenvironment
    • Apoptosis
    • Caspases
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteolytic enzymes
    • Other proteases
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Apoptosis
    • Cancer
    • Cell Death
    • Apoptosis
    • Apoptosis Markers
    • Caspases
    • Cancer
    • Cell Death
    • Necroptosis
    • Cancer
    • Cell Death
    • Apoptosis
    • Metabolism

関連製品

  • Assay kits

    • Caspase-8 Assay Kit (Colorimetric) (ab39700)
    • Caspase-8 (active) FITC Staining Kit (ab65614)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab25901の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.

Human samples only

ICC/IF
Use a concentration of 20 µg/ml.

Human samples only

WB (2)
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
特記事項
ICC
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.

Human samples only

ICC/IF
Use a concentration of 20 µg/ml.

Human samples only

WB
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).

ターゲット情報

  • 機能

    Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death-inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. Cleaves and activates CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10. May participate in the GZMB apoptotic pathways. Cleaves ADPRT. Hydrolyzes the small-molecule substrate, Ac-Asp-Glu-Val-Asp-
    -AMC. Likely target for the cowpox virus CRMA death inhibitory protein. Isoform 5, isoform 6, isoform 7 and isoform 8 lack the catalytic site and may interfere with the pro-apoptotic activity of the complex.
  • 組織特異性

    Isoform 1, isoform 5 and isoform 7 are expressed in a wide variety of tissues. Highest expression in peripheral blood leukocytes, spleen, thymus and liver. Barely detectable in brain, testis and skeletal muscle.
  • 関連疾患

    Defects in CASP8 are the cause of caspase-8 deficiency (CASP8D) [MIM:607271]. CASP8D is a disorder resembling autoimmune lymphoproliferative syndrome (ALPS). It is characterized by lymphadenopathy, splenomegaly, and defective CD95-induced apoptosis of peripheral blood lymphocytes (PBLs). It leads to defects in activation of T-lymphocytes, B-lymphocytes, and natural killer cells leading to immunodeficiency characterized by recurrent sinopulmonary and herpes simplex virus infections and poor responses to immunization.
  • 配列類似性

    Belongs to the peptidase C14A family.
    Contains 2 DED (death effector) domains.
  • ドメイン

    Isoform 9 contains a N-terminal extension that is required for interaction with the BCAP31 complex.
  • 翻訳後修飾

    Generation of the subunits requires association with the death-inducing signaling complex (DISC), whereas additional processing is likely due to the autocatalytic activity of the activated protease. GZMB and CASP10 can be involved in these processing events.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 細胞内局在

    Cytoplasm.
  • Target information above from: UniProt accession Q14790 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 841 Human
    • Entrez Gene: 12370 Mouse
    • Entrez Gene: 64044 Rat
    • Omim: 601763 Human
    • SwissProt: Q14790 Human
    • SwissProt: O89110 Mouse
    • SwissProt: Q9JHX4 Rat
    • Unigene: 599762 Human
    • Unigene: 336851 Mouse
    see all
  • 別名

    • ALPS2B antibody
    • Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 12 protein antibody
    • Apoptosis related cysteine peptidase antibody
    • Apoptotic cysteine protease antibody
    • Apoptotic protease Mch-5 antibody
    • Apoptotic protease Mch5 antibody
    • CAP 4 antibody
    • CAP4 antibody
    • CASP-8 antibody
    • CASP8 antibody
    • CASP8_HUMAN antibody
    • Caspase 8 antibody
    • Caspase 8 apoptosis related cysteine peptidase antibody
    • Caspase IIX antibody
    • Caspase-8 subunit p10 antibody
    • caspase8 antibody
    • CED 3 antibody
    • FADD Homologous ICE/CED3 Like Protease antibody
    • FADD Like ICE antibody
    • FADD-homologous ICE/CED-3-like protease antibody
    • FADD-like ICE antibody
    • FLICE antibody
    • FLJ17672 antibody
    • ICE-like apoptotic protease 5 antibody
    • MACH alpha 1/2/3 protein antibody
    • MACH antibody
    • MACH beta 1/2/3/4 protein antibody
    • MACH5 antibody
    • MCH 5 antibody
    • MCH5 antibody
    • MGC78473 antibody
    • MORT1 associated ced 3 homolog antibody
    • MORT1 associated CED3 homolog antibody
    • MORT1-associated CED-3 homolog antibody
    • OTTHUMP00000163717 antibody
    • OTTHUMP00000163720 antibody
    • OTTHUMP00000163724 antibody
    • OTTHUMP00000163725 antibody
    • OTTHUMP00000165062 antibody
    • OTTHUMP00000165063 antibody
    • OTTHUMP00000165064 antibody
    • OTTHUMP00000206552 antibody
    • OTTHUMP00000206582 antibody
    see all

画像

  • Immunocytochemistry/ Immunofluorescence - Anti-Caspase-8 antibody (ab25901)
    Immunocytochemistry/ Immunofluorescence - Anti-Caspase-8 antibody (ab25901)
    Immunocytochemistry/Immunofluorescence of Jurkat cells labeling Caspase-8 with Anti-Caspase-8 antibody (ab25901) at 20μg/ml.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-8 antibody (ab25901)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-8 antibody (ab25901)

    Immunohistochemistry of Caspase-8 in human spleen tissue with Caspase-8 antibody at 5 µg/ml.

  • Immunocytochemistry/ Immunofluorescence - Anti-Caspase-8 antibody (ab25901)
    Immunocytochemistry/ Immunofluorescence - Anti-Caspase-8 antibody (ab25901)

    ab25901 at 2ug/ml staining Caspase-8 in Jurkat cells by ICC/IF

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-8 antibody (ab25901)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-8 antibody (ab25901)

    IHC image of Caspase-8 staining in human spleen formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab25901, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

プロトコール

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

データシートおよび資料

  • SDS download

  • Datasheet download

    Download

参考文献 (151)

ab25901 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab25901 は 151 報の論文で使用されています。

  • Zhou Y  et al. Receptor-interacting protein 1 inhibition prevents mechanical stress-induced temporomandibular joint osteoarthritis by regulating apoptosis and later-stage necroptosis of chondrocytes. Arch Oral Biol 147:105612 (2023). PubMed: 36603515
  • Lin J  et al. Fas/FasL and Complement Activation are Associated with Chronic Active Epstein-Barr Virus Hepatitis. J Clin Transl Hepatol 11:540-549 (2023). PubMed: 36969885
  • Zhao D  et al. Blocking TRAIL-DR5 signaling pathway with soluble death receptor 5 fusion protein mitigates radiation-induced injury. Front Pharmacol 14:1171293 (2023). PubMed: 37274104
  • Zhang D  et al. Inhibition of GSK3β activity alleviates acute liver failure via suppressing multiple programmed cell death. J Inflamm (Lond) 20:24 (2023). PubMed: 37443080
  • Budijanto S  et al. Dietary supplementation of black rice bran to colon carcinogen-induced mice: Examining the development of colorectal cancer by improving environmental colon conditions. Heliyon 9:e18528 (2023). PubMed: 37576300
View all Publications for this product

レビューと Q&A

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レビューを送る 質問を送る

1-5 of 5 Abreviews or Q&A

Immunohistochemistry (Frozen sections) abreview for Anti-Caspase-8 antibody

Average
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Heart)
Permeabilization
Yes - Triton x-100, 0.01%
Specification
Heart
Blocking step
(agent) for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Dec 01 2017

Western blot abreview for Anti-Caspase 8 antibody

Average
Abreviews
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (stomach cancer, colon cancer)
Loading amount
30 µg
Specification
stomach cancer, colon cancer
Treatment
10uM canabinoid antagonist for 24hrs
Gel Running Conditions
Reduced Denaturing (8% SDS-PAGE)
Blocking step
Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Apr 05 2013

Western blot abreview for Anti-Caspase 8 antibody

Average
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Rat Cell lysate - whole cell (Rat Type II Cell)
Loading amount
20 µg
Specification
Rat Type II Cell
Gel Running Conditions
Reduced Denaturing (8-16% Tris-Glycine)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Aug 28 2009

Question

Multiple bands in Western blot with human and rat samples.

Read More

Abcam community

Verified customer

Asked on Mar 02 2012

Answer

Thanks for your call today and for letting us know about the trouble analyzing the results of ab25901 in Western blot.

As w discussed, I'm sending a vial of ab119892 free of charge on the order ***, which should arrive on Monday. Please keep me updated about the results using this replacement antibody.

I look forward to hearing from you. Let me know if you have any questions, and have a great weekend!

Read More

Abcam Scientific Support

Answered on Mar 02 2012

Question

How much protein was loaded per lane in the western blot shown on your datasheet? What were the conditions used to produce this blot?

Read More

Abcam community

Verified customer

Asked on Jun 15 2011

Answer

Thank you for contacting us. The image on our datasheet was generated using 15ug of Jurkat cell lysate per lane under reduced and denaturing conditions. The membrane was blocked for 2 hours with 5% milk. The antibody was ab25901 was diluted 0.5- 2 ug/mL and incubated overnight at 4C. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Abcam Scientific Support

Answered on Jun 15 2011

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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