Anti-Caspase-8 抗体 (ab25901)
Key features and details
- Rabbit polyclonal to Caspase-8
- Suitable for: ICC, IHC-P, ICC/IF, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Caspase-8 antibody
Caspase-8 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Caspase-8 -
由来種
Rabbit -
アプリケーション
適用あり: ICC, IHC-P, ICC/IF, WBmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide corresponding to Human Caspase-8 (C terminal). Synthetic peptide (Human) - a 16 amino acid peptide from near the carboxy-terminus of human Caspase-8 isoform A.
Database link: Q14790 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at +4°C. -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Caspase-8 Antibody is affinity chromatography purified via peptide column. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Assay kits
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab25901の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC |
Use at an assay dependent concentration.
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| IHC-P |
Use at an assay dependent concentration.
Human samples only |
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| ICC/IF |
Use a concentration of 20 µg/ml.
Human samples only |
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| WB | (2) |
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
|
| 特記事項 |
|---|
|
ICC
Use at an assay dependent concentration. |
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IHC-P
Use at an assay dependent concentration. Human samples only |
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ICC/IF
Use a concentration of 20 µg/ml. Human samples only |
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WB
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa). |
ターゲット情報
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機能
Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death-inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. Cleaves and activates CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10. May participate in the GZMB apoptotic pathways. Cleaves ADPRT. Hydrolyzes the small-molecule substrate, Ac-Asp-Glu-Val-Asp-
-AMC. Likely target for the cowpox virus CRMA death inhibitory protein. Isoform 5, isoform 6, isoform 7 and isoform 8 lack the catalytic site and may interfere with the pro-apoptotic activity of the complex. -
組織特異性
Isoform 1, isoform 5 and isoform 7 are expressed in a wide variety of tissues. Highest expression in peripheral blood leukocytes, spleen, thymus and liver. Barely detectable in brain, testis and skeletal muscle. -
関連疾患
Defects in CASP8 are the cause of caspase-8 deficiency (CASP8D) [MIM:607271]. CASP8D is a disorder resembling autoimmune lymphoproliferative syndrome (ALPS). It is characterized by lymphadenopathy, splenomegaly, and defective CD95-induced apoptosis of peripheral blood lymphocytes (PBLs). It leads to defects in activation of T-lymphocytes, B-lymphocytes, and natural killer cells leading to immunodeficiency characterized by recurrent sinopulmonary and herpes simplex virus infections and poor responses to immunization. -
配列類似性
Belongs to the peptidase C14A family.
Contains 2 DED (death effector) domains. -
ドメイン
Isoform 9 contains a N-terminal extension that is required for interaction with the BCAP31 complex. -
翻訳後修飾
Generation of the subunits requires association with the death-inducing signaling complex (DISC), whereas additional processing is likely due to the autocatalytic activity of the activated protease. GZMB and CASP10 can be involved in these processing events.
Phosphorylated upon DNA damage, probably by ATM or ATR. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 841 Human
- Entrez Gene: 12370 Mouse
- Entrez Gene: 64044 Rat
- Omim: 601763 Human
- SwissProt: Q14790 Human
- SwissProt: O89110 Mouse
- SwissProt: Q9JHX4 Rat
- Unigene: 599762 Human
see all -
別名
- ALPS2B antibody
- Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 12 protein antibody
- Apoptosis related cysteine peptidase antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Anti-Caspase-8 antibody (ab25901)Immunocytochemistry/Immunofluorescence of Jurkat cells labeling Caspase-8 with Anti-Caspase-8 antibody (ab25901) at 20μg/ml.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-8 antibody (ab25901)Immunohistochemistry of Caspase-8 in human spleen tissue with Caspase-8 antibody at 5 µg/ml.
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Western blot - Anti-Caspase-8 antibody (ab25901)All lanes : Anti-Caspase-8 antibody (ab25901) at 0.5 µg/ml
Lane 1 : Human spleen
Lane 2 : Mouse spleen
Lane 3 : Rat spleen
Predicted band size: 55 kDa -
Western blot - Anti-Caspase-8 antibody (ab25901)All lanes : Anti-Caspase-8 antibody (ab25901) at 1 µg/ml
Lane 1 : Jurkat cell lysates with Absence of blocking buffer
Lane 2 : Jurkat cell lysates with Presence of blocking buffer
Predicted band size: 55 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-Caspase-8 antibody (ab25901)ab25901 at 2ug/ml staining Caspase-8 in Jurkat cells by ICC/IF
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-8 antibody (ab25901)IHC image of Caspase-8 staining in human spleen formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab25901, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (136)
ab25901 は 136 報の論文で使用されています。
- Ou D et al. Galectin-1 alleviates myocardial ischemia-reperfusion injury by reducing the inflammation and apoptosis of cardiomyocytes. Exp Ther Med 23:143 (2022). PubMed: 35069824
- Sercundes MK et al. Blockade of caspase cascade overcomes malaria-associated acute respiratory distress syndrome in mice. Cell Death Dis 13:144 (2022). PubMed: 35145061
- Lu Z et al. Promotion of microRNA-146a by histone deacetylase 4 silencing contributes to radiosensitization of esophageal carcinoma. J Transl Med 20:101 (2022). PubMed: 35193602
- Li X et al. Overexpression of TP53INP2 Promotes Apoptosis in Clear Cell Renal Cell Cancer via Caspase-8/TRAF6 Signaling Pathway. J Immunol Res 2022:1260423 (2022). PubMed: 35615533
- Jeong JY et al. Trifluoperazine and Its Analog Suppressed the Tumorigenicity of Non-Small Cell Lung Cancer Cell; Applicability of Antipsychotic Drugs to Lung Cancer Treatment. Biomedicines 10:N/A (2022). PubMed: 35625784