Anti-Caspase-7 抗体 (ab25900)
Key features and details
- Rabbit polyclonal to Caspase-7
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Caspase-7 antibody
Caspase-7 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Caspase-7 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human Caspase-7 aa 1-16 (C terminal).
Database link: P55210 -
ポジティブ・コントロール
- Jurkat cell lysate, human skeletal muscle cell
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab25900の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
IHC-P |
Use a concentration of 2 µg/ml.
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WB | (1) |
Use a concentration of 0.5 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 34 kDa).
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特記事項 |
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IHC-P
Use a concentration of 2 µg/ml. |
WB
Use a concentration of 0.5 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 34 kDa). |
ターゲット情報
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機能
Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-
-Gly-217' bond. Overexpression promotes programmed cell death. -
組織特異性
Highly expressed in lung, skeletal muscle, liver, kidney, spleen and heart, and moderately in testis. No expression in the brain. -
配列類似性
Belongs to the peptidase C14A family. -
翻訳後修飾
Cleavages by granzyme B or caspase-10 generate the two active subunits. Propeptide domains can also be cleaved efficiently by caspase-3. Active heterodimers between the small subunit of caspase-7 and the large subunit of caspase-3, and vice versa, also occur. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 840 Human
- Omim: 601761 Human
- SwissProt: P55210 Human
- Unigene: 9216 Human
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別名
- Apoptotic protease Mch-3 antibody
- Apoptotic protease MCH3 antibody
- CASP-7 antibody
see all
画像
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Lane 1 : Anti-Caspase-7 antibody (ab25900) at 0.5 µg/ml
Lane 2 : Anti-Caspase-7 antibody (ab25900) at 1 µg/ml
Lane 3 : Anti-Caspase-7 antibody (ab25900) at 2 µg/ml
All lanes : Human skeletal
muscle cell lysate
Predicted band size: 34 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-7 antibody (ab25900)
Immunohistochemical staining of human skeletal muscle (paraffin embedded sections) using ab25900 anti Caspase-7 at 2 µg/ml.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (8)
ab25900 は 8 報の論文で使用されています。
- Meng L et al. Costunolide attenuates oxygen-glucose deprivation/reperfusion-induced mitochondrial-mediated apoptosis in PC12 cells. Mol Med Rep 23:N/A (2021). PubMed: 33786628
- Subhawa S et al. The Effects of Houttuynia cordata Thunb and Piper ribesioides Wall Extracts on Breast Carcinoma Cell Proliferation, Migration, Invasion and Apoptosis. Molecules 25:N/A (2020). PubMed: 32155880
- Zhao Y et al. Cortistatin binds to TNF-a receptors and protects against osteoarthritis. EBioMedicine 41:556-570 (2019). PubMed: 30826358
- Chen J et al. SM-164 enhances the antitumor activity of adriamycin in human U2-OS cells via downregulation of X-linked inhibitor of apoptosis protein. Mol Med Rep 19:5079-5086 (2019). PubMed: 31059038
- Yin Y et al. Expression of miRNA-214 in the sera of elderly patients with acute myocardial infarction and its effect on cardiomyocyte apoptosis. Exp Ther Med 17:4657-4662 (2019). PubMed: 31086597
- Zhao Q et al. Protective effects of dehydrocostuslactone on rat hippocampal slice injury induced by oxygen-glucose deprivation/reoxygenation. Int J Mol Med 42:1190-1198 (2018). PubMed: 29786748
- Chen Q et al. USP10 promotes proliferation and migration and inhibits apoptosis of endometrial stromal cells in endometriosis through activating the Raf-1/MEK/ERK pathway. Am J Physiol Cell Physiol 315:C863-C872 (2018). PubMed: 30281322
- Ma J et al. Reversing drug resistance of soft tumor-repopulating cells by tumor cell-derived chemotherapeutic microparticles. Cell Res 26:713-27 (2016). PubMed: 27167569