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リコンビナントRabMAb

Anti-Caspase-6/CASP-6 抗体 [EPR18046] (ab185646)

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Western blot - Anti-Caspase-6/CASP-6 antibody [EPR18046] (ab185646)
  • Western blot - Anti-Caspase-6/CASP-6 antibody [EPR18046] (ab185646)
  • Western blot - Anti-Caspase-6/CASP-6 antibody [EPR18046] (ab185646)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-6/CASP-6 antibody [EPR18046] (ab185646)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-6/CASP-6 antibody [EPR18046] (ab185646)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-6/CASP-6 antibody [EPR18046] (ab185646)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-6/CASP-6 antibody [EPR18046] (ab185646)
  • Anti-Caspase-6/CASP-6 antibody [EPR18046] (ab185646)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18046] to Caspase-6/CASP-6
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-Caspase-6/CASP-6 antibody [EPR18046]
    Caspase-6/CASP-6 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR18046] to Caspase-6/CASP-6

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-Pmore details

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Jurkat whole cell lysates untreated and treated with 1uM staurosporine for 4 hours; Mouse heart, mouse kidney, mouse spleen, rat brain and rat kidney lysates; RAW 264.7 whole cell lysate. IHC-P: Human colon, Human endometrium carcinoma, mouse kidney and rat kidney.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab185646の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/2000. Detects a band of approximately 33,11 kDa (predicted molecular weight: 33 kDa).
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
特記事項
WB
1/2000. Detects a band of approximately 33,11 kDa (predicted molecular weight: 33 kDa).
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ターゲット情報

  • 機能

    Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves poly(ADP-ribose) polymerase in vitro, as well as lamins. Overexpression promotes programmed cell death.

  • 配列類似性

    Belongs to the peptidase C14A family.

  • 翻訳後修飾

    Cleavages by caspase-3, caspase-8 or -10 generate the two active subunits.

  • 細胞内局在

    Cytoplasm.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • Apoptotic protease Mch-2 antibody
    • Apoptotic protease MCH2 antibody
    • CASP-6 antibody
    • CASP6 antibody
    • CASP6_HUMAN antibody
    • Caspase 6 antibody
    • Caspase 6 apoptosis related cysteine protease antibody
    • Caspase 6, apoptosis related cysteine peptidase antibody
    • Caspase-6 antibody
    • Caspase-6 subunit p11 antibody
    • Mch2 antibody
    • OTTHUMP00000162742 antibody
    • OTTHUMP00000162743 antibody
    see all

画像

  • Western blot - Anti-Caspase-6/CASP-6 antibody [EPR18046] (ab185646)
    Western blot - Anti-Caspase-6/CASP-6 antibody [EPR18046] (ab185646)
    All lanes : Anti-Caspase-6/CASP-6 antibody [EPR18046] (ab185646) at 1/2000 dilution

    Lane 1 : Untreated Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysates
    Lane 2 : Jurkat whole cell lysates treated with 1uM staurosporine for 4 hours

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 33 kDa
    Observed band size: 11,33 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Caspase-6/CASP-6 antibody [EPR18046] (ab185646)
    Western blot - Anti-Caspase-6/CASP-6 antibody [EPR18046] (ab185646)
    All lanes : Anti-Caspase-6/CASP-6 antibody [EPR18046] (ab185646) at 1/2000 dilution

    Lane 1 : Mouse heart lysates
    Lane 2 : Mouse kidney lysates
    Lane 3 : Mouse spleen lysates
    Lane 4 : Rat brain lysates
    Lane 5 : Rat kidney lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 33 kDa
    Observed band size: 33 kDa


    Exposure time: 1 minute


    The predicted MW is 32kDa for mouse and rat full-length procaspase-6.

    Blocking/Dilution buffer: 5% NFDM/TBST.

     

  • Western blot - Anti-Caspase-6/CASP-6 antibody [EPR18046] (ab185646)
    Western blot - Anti-Caspase-6/CASP-6 antibody [EPR18046] (ab185646)
    Anti-Caspase-6/CASP-6 antibody [EPR18046] (ab185646) at 1/2000 dilution + RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg

    Secondary
    Goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

    Predicted band size: 33 kDa
    Observed band size: 11,33 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    The predicted MW is 32kDa for mouse and rat full-length procaspase-6.

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-6/CASP-6 antibody [EPR18046] (ab185646)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-6/CASP-6 antibody [EPR18046] (ab185646)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Caspase-6/CASP-6 with ab185646 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Nuclear and cytoplasmic staining on the epithelial cells of Human colon is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-6/CASP-6 antibody [EPR18046] (ab185646)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-6/CASP-6 antibody [EPR18046] (ab185646)

    Immunohistochemical analysis of paraffin-embedded Human endometrium carcinoma tissue labeling Caspase-6/CASP-6 with ab185646 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Nuclear and cytoplasmic staining on the cancer cells of endometrium carcinoma is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-6/CASP-6 antibody [EPR18046] (ab185646)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-6/CASP-6 antibody [EPR18046] (ab185646)

    Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling Caspase-6/CASP-6 with ab185646 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasmic and nuclear staining on mouse kidney is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-6/CASP-6 antibody [EPR18046] (ab185646)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-6/CASP-6 antibody [EPR18046] (ab185646)

    Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling Caspase-6/CASP-6 with ab185646 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasmic and nuclear staining on rat kidney is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-Caspase-6/CASP-6 antibody [EPR18046] (ab185646)
    Anti-Caspase-6/CASP-6 antibody [EPR18046] (ab185646)

参考文献 (0)

ab185646 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab185646 は論文での使用が確認できていません。

レビューと Q&A

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レビューを送る 質問を送る

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Caspase-6 / CASP-6 antibody [EPR18046]

 Inconclusive
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Tonsil)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Tonsil
Blocking step
BSA as blocking agent for 1 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Carl Hobbs

Verified customer

投稿 Feb 13 2017

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