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リコンビナントRabMAb

Anti-Caspase-6/CASP-6 抗体 [EPR18043] (ab185645)

Reviews (1) Submit a question References (15)
Western blot - Anti-Caspase-6/CASP-6 antibody [EPR18043] (ab185645)
  • Western blot - Anti-Caspase-6/CASP-6 antibody [EPR18043] (ab185645)
  • Western blot - Anti-Caspase-6/CASP-6 antibody [EPR18043] (ab185645)
  • Immunocytochemistry/ Immunofluorescence - Anti-Caspase-6/CASP-6 antibody [EPR18043] (ab185645)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-6/CASP-6 antibody [EPR18043] (ab185645)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-6/CASP-6 antibody [EPR18043] (ab185645)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-6/CASP-6 antibody [EPR18043] (ab185645)
  • Immunoprecipitation - Anti-Caspase-6/CASP-6 antibody [EPR18043] (ab185645)
  • Anti-Caspase-6/CASP-6 antibody [EPR18043] (ab185645)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18043] to Caspase-6/CASP-6
  • Suitable for: WB, IHC-P, IP, ICC/IF
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-Caspase-6/CASP-6 antibody [EPR18043]
    Caspase-6/CASP-6 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR18043] to Caspase-6/CASP-6

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-P, IP, ICC/IFmore details

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Jurkat, NIH/3T3, C6 and PC-12 whole cell lysates. IHC-P: Human colon, mouse brain and rat brain tissues. ICC/IF: Jurkat cells. IP: NIH/3T3 whole cell lysate treated with 1uM staurosporine for 4 hours.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab185645の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000. Detects a band of approximately 33, 11 kDa (predicted molecular weight: 33 kDa).
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP
1/50.
ICC/IF
1/500.
特記事項
WB
1/1000. Detects a band of approximately 33, 11 kDa (predicted molecular weight: 33 kDa).
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP
1/50.
ICC/IF
1/500.

ターゲット情報

  • 機能

    Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves poly(ADP-ribose) polymerase in vitro, as well as lamins. Overexpression promotes programmed cell death.

  • 配列類似性

    Belongs to the peptidase C14A family.

  • 翻訳後修飾

    Cleavages by caspase-3, caspase-8 or -10 generate the two active subunits.

  • 細胞内局在

    Cytoplasm.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • Apoptotic protease Mch-2 antibody
    • Apoptotic protease MCH2 antibody
    • CASP-6 antibody
    • CASP6 antibody
    • CASP6_HUMAN antibody
    • Caspase 6 antibody
    • Caspase 6 apoptosis related cysteine protease antibody
    • Caspase 6, apoptosis related cysteine peptidase antibody
    • Caspase-6 antibody
    • Caspase-6 subunit p11 antibody
    • Mch2 antibody
    • OTTHUMP00000162742 antibody
    • OTTHUMP00000162743 antibody
    see all

画像

  • Western blot - Anti-Caspase-6/CASP-6 antibody [EPR18043] (ab185645)
    Western blot - Anti-Caspase-6/CASP-6 antibody [EPR18043] (ab185645)
    All lanes : Anti-Caspase-6/CASP-6 antibody [EPR18043] (ab185645) at 1/1000 dilution

    Lane 1 : Untreated Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate
    Lane 2 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate treated with 1uM staurosporine for 4 hours

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 33 kDa
    Observed band size: 11,33 kDa why is the actual band size different from the predicted?


    Exposure time: 1 minute


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Caspase-6/CASP-6 antibody [EPR18043] (ab185645)
    Western blot - Anti-Caspase-6/CASP-6 antibody [EPR18043] (ab185645)
    All lanes : Anti-Caspase-6/CASP-6 antibody [EPR18043] (ab185645) at 1/1000 dilution

    Lane 1 : Untreated NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate
    Lane 2 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate treated with 1uM staurosporine for 4 hours

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 33 kDa
    Observed band size: 11,33 kDa why is the actual band size different from the predicted?


    Exposure time: 1 minute


    The predicted MW is 32kDa for mouse and rat full-length procaspase-6.

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Caspase-6/CASP-6 antibody [EPR18043] (ab185645)
    Western blot - Anti-Caspase-6/CASP-6 antibody [EPR18043] (ab185645)
    All lanes : Anti-Caspase-6/CASP-6 antibody [EPR18043] (ab185645) at 1/1000 dilution

    Lane 1 : C6 (Rat glial tumor cells) whole cell lysate
    Lane 2 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 33 kDa
    Observed band size: 33 kDa


    Exposure time: 1 minute


    The predicted MW is 32kDa for mouse and rat full-length procaspase-6.

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunocytochemistry/ Immunofluorescence - Anti-Caspase-6/CASP-6 antibody [EPR18043] (ab185645)
    Immunocytochemistry/ Immunofluorescence - Anti-Caspase-6/CASP-6 antibody [EPR18043] (ab185645)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling Caspase-6/CASP-6 with ab185645 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green).

    Confocal image showing both nuclear and cytoplasmic staining on Jurkat cells. The staining remained similar after treatment with staurosporine (1uM, 4 hours) as the antibody interacts with the subunit p11. 

    The nuclear counter stain is DAPI (blue).

    Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
    The negative controls are as follows:
    -ve control 1: ab185645 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-6/CASP-6 antibody [EPR18043] (ab185645)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-6/CASP-6 antibody [EPR18043] (ab185645)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Caspase-6/CASP-6 with ab185645 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution.

    Cytoplasmic and nuclear staining on epithelial cells of human colon is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-6/CASP-6 antibody [EPR18043] (ab185645)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-6/CASP-6 antibody [EPR18043] (ab185645)

    Immunohistochemical analysis of paraffin-embedded Mouse brain tissue labeling Caspase-6/CASP-6 with ab185645 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution.

    Cytoplasmic staining on neurons of mouse brain is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-6/CASP-6 antibody [EPR18043] (ab185645)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-6/CASP-6 antibody [EPR18043] (ab185645)

    Immunohistochemical analysis of paraffin-embedded Rat brain tissue labeling Caspase-6/CASP-6 with ab185645 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution.

    Cytoplasmic staining on neurons of rat brain is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-Caspase-6/CASP-6 antibody [EPR18043] (ab185645)
    Immunoprecipitation - Anti-Caspase-6/CASP-6 antibody [EPR18043] (ab185645)

    Caspase-6/CASP-6 was immunoprecipitated from 1mg of NIH/3T3 (Mouse embyro fibroblast cells) treated with 1uM staurosporine for 4 hours whole cell lysate with ab185645 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab185645 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/1500 dilution.

    Lane 1: NIH/3T3 treated with 1uM staurosporine for 4 hours whole cell lysate10 µg (Input). Lane 2: ab185645 IP in NIH/3T3 treated with 1uM staurosporine for 4 hours whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab185645 in NIH/3T3 treated with 1uM staurosporine for 4 hours whole cell lysate.

    The cleaved Caspase-6/CASP-6 appears slightly larger than 11kDa. This fragment contains subunit p11 plus the internal propeptide.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

  • Anti-Caspase-6/CASP-6 antibody [EPR18043] (ab185645)
    Anti-Caspase-6/CASP-6 antibody [EPR18043] (ab185645)

参考文献 (15)

ab185645 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab185645 は 15 報の論文で使用されています。

  • Sheng M  et al. Caspase 6/NR4A1/SOX9 signaling axis regulates hepatic inflammation and pyroptosis in ischemia-stressed fatty liver. Cell Death Discov 9:106 (2023). PubMed: 36977670
  • Chu H  et al. Coronaviruses exploit a host cysteine-aspartic protease for replication. Nature 609:785-792 (2022). PubMed: 35922005
  • Farooq J  et al. RNA Binding Motif 5 Gene Deletion Modulates Cell Signaling in a Sex-Dependent Manner but Not Hippocampal Cell Death. J Neurotrauma 39:577-589 (2022). PubMed: 35152732
  • Wang ZY  et al. Negative feedback of SNRK to circ-SNRK regulates cardiac function post-myocardial infarction. Cell Death Differ 29:709-721 (2022). PubMed: 34621049
  • Wu W  et al. Caspase-Dependent Cleavage of DDX21 Suppresses Host Innate Immunity. mBio 12:e0100521 (2021). PubMed: 34125604
View all Publications for this product

レビューと Q&A

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レビューを送る 質問を送る

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Caspase-6/CASP-6 antibody [EPR18043]

 Inconclusive
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Tonsil)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Tonsil
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Carl Hobbs

Verified customer

投稿 Feb 13 2017

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