Anti-Caspase-6/CASP-6 抗体 [E180] (ab32366)
![Western blot - Anti-Caspase-6/CASP-6 antibody [E180] (ab32366)](https://www.abcam.co.jp/ps/products/32/ab32366/Images/ab32366-44582-anti-caspase-6-casp-6-antibody-e180-western-blot.jpg "Western blot - Anti-Caspase-6/CASP-6 antibody [E180] (ab32366)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [E180] to Caspase-6/CASP-6
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Caspase-6/CASP-6 antibody [E180]
Caspase-6/CASP-6 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [E180] to Caspase-6/CASP-6 -
由来種
Rabbit -
特異性
This antibody detects the pro form and the p11 subunit of human Caspase-6/CASP-6. We have data to indicate that this antibody may not cross react with Rat. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
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アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details
適用なし: IP -
種交差性
交差種: Human
交差が予測される動物種: Mouse
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免疫原
Synthetic peptide within Human Caspase-6/CASP-6 aa 250-350 (C terminal). The exact sequence is proprietary.
Database link: P55212 -
ポジティブ･コントロール
- WB: Jurkat cell lysate. ICC/IF: Hela cells. IHC-P: Human lung carcinoma tissue slides.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
E180 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab32366の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 30 kDa (predicted molecular weight: 34 kDa).
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| IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| ICC/IF |
1/100.
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| 特記事項 |
|---|
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WB
1/1000. Detects a band of approximately 30 kDa (predicted molecular weight: 34 kDa). |
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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ICC/IF
1/100. |
ターゲット情報
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機能
Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves poly(ADP-ribose) polymerase in vitro, as well as lamins. Overexpression promotes programmed cell death. -
配列類似性
Belongs to the peptidase C14A family. -
翻訳後修飾
Cleavages by caspase-3, caspase-8 or -10 generate the two active subunits. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 839 Human
- Entrez Gene: 12368 Mouse
- Omim: 601532 Human
- SwissProt: P55212 Human
- SwissProt: O08738 Mouse
- Unigene: 654616 Human
- Unigene: 281379 Mouse
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別名
- Apoptotic protease Mch-2 antibody
- Apoptotic protease MCH2 antibody
- CASP-6 antibody
see all
画像
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Western blot - Anti-Caspase-6/CASP-6 antibody [E180] (ab32366)All lanes : Anti-Caspase-6/CASP-6 antibody [E180] (ab32366) at 1/1000 dilution
Lane 1 : Untreated Jurkat cell lysate.
Lane 2 : Jurkat cell lysate treated with Camptothecin.
Predicted band size: 34 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted? -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-6/CASP-6 antibody [E180] (ab32366)](https://www.abcam.co.jp/ps/products/32/ab32366/Images/ab32366-44580-anti-caspase-6-casp-6-antibody-e180-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-6/CASP-6 antibody [E180] (ab32366)Ab32366, at a 1:50 dilution, staining Caspase-6/CASP-6 in paraffin embedded human lung adenocarcinoma tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-Caspase-6/CASP-6 antibody [E180] (ab32366)](https://www.abcam.co.jp/ps/products/32/ab32366/Images/ab32366-44581-anti-caspase-6-casp-6-antibody-e180-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Caspase-6/CASP-6 antibody [E180] (ab32366)Ab32366, at a 1:100 dilution, staining Caspase-6/CASP-6 in Hela cells by Immunocytochemistry/Immunofluorescence.
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![Anti-Caspase-6/CASP-6 antibody [E180] (ab32366)](https://www.abcam.co.jp/ps/products/32/ab32366/Images/ab32366-4-benefits-of-recombinant-antibodies.png)
Anti-Caspase-6/CASP-6 antibody [E180] (ab32366)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab32366 は 1 報の論文で使用されています。
- Coutinho-Camillo CM et al. Caspase expression in oral squamous cell carcinoma. Head Neck 33:1191-8 (2011). IHC ; Human . PubMed: 21755562