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リコンビナントRabMAb

Anti-Caspase-3 p12 抗体 [EPR16888] (ab179517)

Submit a review Submit a question References (23)
Western blot - Anti-Caspase-3 p12 antibody [EPR16888] (ab179517)
  • Western blot - Anti-Caspase-3 p12 antibody [EPR16888] (ab179517)
  • Western blot - Anti-Caspase-3 p12 antibody [EPR16888] (ab179517)
  • Flow Cytometry (Intracellular) - Anti-Caspase-3 p12 antibody [EPR16888] (ab179517)
  • Immunocytochemistry/ Immunofluorescence - Anti-Caspase-3 p12 antibody [EPR16888] (ab179517)
  • Western blot - Anti-Caspase-3 p12 antibody [EPR16888] (ab179517)
  • Western blot - Anti-Caspase-3 p12 antibody [EPR16888] (ab179517)
  • Western blot - Anti-Caspase-3 p12 antibody [EPR16888] (ab179517)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-3 p12 antibody [EPR16888] (ab179517)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-3 p12 antibody [EPR16888] (ab179517)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-3 p12 antibody [EPR16888] (ab179517)
  • Anti-Caspase-3 p12 antibody [EPR16888] (ab179517)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16888] to Caspase-3 p12
  • Suitable for: WB, IHC-P, ICC/IF, Flow Cyt (Intra)
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-Caspase-3 p12 antibody [EPR16888]
    Caspase-3 p12 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR16888] to Caspase-3 p12

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-P, ICC/IF, Flow Cyt (Intra)more details

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: HeLa, PC12 and NIH/3T3 whole cell lysates. Mouse and Rat brain, hippocampus,spinal cord, cerebellum, cerebral cortex, liver and heart tissue lysates, human brain, liver, hypothalamus, heart and cerebellum tissue lysates. IHC-P: Human tonsil, mouse testis and rat kidney tissues. ICC/IF: K562 cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • バッファー

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR16888

  • アイソタイプ

    IgG

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab179517の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000. Detects a band of approximately 32 ,29, 12 kDa (predicted molecular weight: 32, 29, 12 kDa).
IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
1/150.
Flow Cyt (Intra)
Use at an assay dependent concentration.
特記事項
WB
1/1000. Detects a band of approximately 32 ,29, 12 kDa (predicted molecular weight: 32, 29, 12 kDa).
IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
1/150.
Flow Cyt (Intra)
Use at an assay dependent concentration.

ターゲット情報

  • 機能

    Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-
    -Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage.

  • 組織特異性

    Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.

  • 配列類似性

    Belongs to the peptidase C14A family.

  • 翻訳後修飾

    Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa.
    S-nitrosylated on its catalytic site cysteine in unstimulated human cell lines and denitrosylated upon activation of the Fas apoptotic pathway, associated with an increase in intracellular caspase activity. Fas therefore activates caspase-3 not only by inducing the cleavage of the caspase zymogen to its active subunits, but also by stimulating the denitrosylation of its active site thiol.

  • 細胞内局在

    Cytoplasm.
  • Information by UniProt

  • 参照データベース

    • 製品の状態

      Caspase-3 p12 is from aa 176-277 of P42574.

    • 別名

      • Apopain antibody
      • CASP-3 antibody
      • CASP3 antibody
      • CASP3_HUMAN antibody
      • Caspase 3 p12 antibody
      • Caspase-3 subunit p12 antibody
      • CPP-32 antibody
      • Cysteine protease CPP32 antibody
      • Protein Yama antibody
      • SCA-1 antibody
      • SREBP cleavage activity 1 antibody
      see all

    画像

    • Western blot - Anti-Caspase-3 p12 antibody [EPR16888] (ab179517)
      Western blot - Anti-Caspase-3 p12 antibody [EPR16888] (ab179517)
      All lanes : Anti-Caspase-3 p12 antibody [EPR16888] (ab179517) at 1/1000 dilution

      Lane 1 : Mouse Alzheimer's disease brain tissue lysate
      Lane 2 : Mouse brain cancer tissue lysate
      Lane 3 : Mouse hippocampus tissue lysate
      Lane 4 : Mouse spinal cord tissue lysate
      Lane 5 : Mouse cerebellum tissue lysate
      Lane 6 : Mouse cerebral cortex tissue lysate
      Lane 7 : Mouse hypothalamus tissue lysate
      Lane 8 : Mouse heart tissue lysate
      Lane 9 : Mouse liver tissue lysate
      Lane 10 : Human brain tissue lysate
      Lane 11 : Human liver tissue lysate
      Lane 12 : Human hypothalamus tissue lysate
      Lane 13 : Human heart tissue lysate
      Lane 14 : Human cerebellum tissue

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

      Predicted band size: 32, 29, 12 kDa
      Observed band size: 27-32 kDa why is the actual band size different from the predicted?


      Exposure time: 3 seconds


      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

      ab181602 was used as loading control.

      Bands between 27-32kDa represent cleavage of the procaspase at D9 and D28, respectively (PMID: 14567691)

    • Western blot - Anti-Caspase-3 p12 antibody [EPR16888] (ab179517)
      Western blot - Anti-Caspase-3 p12 antibody [EPR16888] (ab179517)
      All lanes : Anti-Caspase-3 p12 antibody [EPR16888] (ab179517) at 1/1000 dilution

      Lane 1 : Rat brain tissue lysate
      Lane 2 : Rat hippocampus tissue lysate
      Lane 3 : Rat spinal cord tissue lysate
      Lane 4 : Rat cerebellum tissue lysate
      Lane 5 : Rat cerebral cortex tissue
      Lane 6 : Rat hypothalamus tissue
      Lane 7 : Rat heart tissue lysate
      Lane 8 : Rat liver tissue lysate
      Lane 9 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

      Predicted band size: 32, 29, 12 kDa
      Observed band size: 27-32 kDa why is the actual band size different from the predicted?


      Exposure time: 5 seconds


      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

      ab181602 was used as loading control.

      Bands between 27-32kDa represent cleavage of the procaspase at D9 and D28, respectively (PMID: 14567691)

    • Western blot - Anti-Caspase-3 p12 antibody [EPR16888] (ab179517)
      Western blot - Anti-Caspase-3 p12 antibody [EPR16888] (ab179517)
      All lanes : Anti-Caspase-3 p12 antibody [EPR16888] (ab179517) at 1/1000 dilution

      Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysates treated with staurosporine 1uM for 4hr
      Lane 2 : HeLa untreated whole cell lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 32, 29, 12 kDa
      Observed band size: 12,29,32 kDa why is the actual band size different from the predicted?



      Blocking/Dilution buffer: 5% NFDM/TBST.

       

      32 and 29 kDa bands represent procaspase-3; 12-kDa band is Caspase-3 subunit p12.

    • Flow Cytometry (Intracellular) - Anti-Caspase-3 p12 antibody [EPR16888] (ab179517)
      Flow Cytometry (Intracellular) - Anti-Caspase-3 p12 antibody [EPR16888] (ab179517)

      Intracellular Flow Cytometry analysis of K562 (human chronic myelogenous leukemia) with purified ab179517 at 1/240 dilution (Red). The secondary antibody was Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution. A Rabbit monoclonal IgG (Black) was used as the isotype control and cells without incubation with primary antibody and secondary antibody (Blue) were used as unlabeled control. 

       

       

    • Immunocytochemistry/ Immunofluorescence - Anti-Caspase-3 p12 antibody [EPR16888] (ab179517)
      Immunocytochemistry/ Immunofluorescence - Anti-Caspase-3 p12 antibody [EPR16888] (ab179517)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized K562 (Human chronic myelogenous leukemia cells from bone marrow) cells labeling Caspase-3 p12 with ab179517 at 1/150 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/400 dilution (green). Confocal image showing cytoplasmic staining on K562 cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
      The negative controls are as follows;
      -ve control 1: ab179517 at 1/150 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
      -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/400 dilution.

       

    • Western blot - Anti-Caspase-3 p12 antibody [EPR16888] (ab179517)
      Western blot - Anti-Caspase-3 p12 antibody [EPR16888] (ab179517)
      All lanes : Anti-Caspase-3 p12 antibody [EPR16888] (ab179517) at 1/1000 dilution

      Lane 1 : NIH/3T3 (Mouse embyro fibroblast cells) treated with staurosporine 1uM for 4h
      Lane 2 : Untreated NIH/3T3 whole cell lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 32, 29, 12 kDa
      Observed band size: 12,29,32 kDa why is the actual band size different from the predicted?



      Blocking/Dilution buffer: 5% NFDM/TBST.

       

      32 and 29 kDa bands represent procaspase-3; 12-kDa band is Caspase-3 subunit p12.

    • Western blot - Anti-Caspase-3 p12 antibody [EPR16888] (ab179517)
      Western blot - Anti-Caspase-3 p12 antibody [EPR16888] (ab179517)
      Anti-Caspase-3 p12 antibody [EPR16888] (ab179517) at 1/5000 dilution + Mouse spleen lysates at 10 µg

      Secondary
      Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 32, 29, 12 kDa
      Observed band size: 29,32 kDa why is the actual band size different from the predicted?



      Blocking/Dilution buffer: 5% NFDM/TBST.

       

      32 and 29 kDa bands represent procaspase-3.

    • Western blot - Anti-Caspase-3 p12 antibody [EPR16888] (ab179517)
      Western blot - Anti-Caspase-3 p12 antibody [EPR16888] (ab179517)
      All lanes : Anti-Caspase-3 p12 antibody [EPR16888] (ab179517) at 1/1000 dilution

      Lane 1 : Mouse brain lysates
      Lane 2 : Mouse heart lysates
      Lane 3 : Rat brain lysates
      Lane 4 : Rat heart lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 32, 29, 12 kDa
      Observed band size: 29,32 kDa why is the actual band size different from the predicted?



      Blocking/Dilution buffer: 5% NFDM/TBST.

       

      32 and 29 kDa bands represent procaspase-3.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-3 p12 antibody [EPR16888] (ab179517)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-3 p12 antibody [EPR16888] (ab179517)

      Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling Caspase-3 p12 with ab179517 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) secondary antibody (ab97051) at 1/500 dilution. Cytoplasmic and nuclear staining in germinal center, weaker staining in the mantle zone (MZ) lymphocytes is observed. Counter stained with Hematoxylin.

      Negative control: Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) secondary antibody (ab97051) at 1/500 dilution.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-3 p12 antibody [EPR16888] (ab179517)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-3 p12 antibody [EPR16888] (ab179517)

      Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling Caspase-3 p12 with ab179517 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) secondary antibody (ab97051) at 1/500 dilution. Nuclear and cytoplasmic staining on leydig cells and spermatogonial cells is observed. Counter stained with Hematoxylin.

      Negative control: Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) secondary antibody (ab97051) at 1/500 dilution.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-3 p12 antibody [EPR16888] (ab179517)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-3 p12 antibody [EPR16888] (ab179517)

      Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling Caspase-3 p12 with ab179517 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) secondary antibody (ab97051) at 1/500 dilution. Granular staining on epithelium from kidney is observed. Counter stained with Hematoxylin.

      Negative control: Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) secondary antibody (ab97051) at 1/500 dilution.

       

      [1] Afshin Samali, Boris Zhivotovsky, Dean P. Jonesa, Sten Orreniusa. Detection of pro-caspase-3 in cytosol and mitochondria of various tissues (rat). FEBS Letters 431 (1998) 167-169.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Anti-Caspase-3 p12 antibody [EPR16888] (ab179517)
      Anti-Caspase-3 p12 antibody [EPR16888] (ab179517)

    参考文献 (23)

    ab179517 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab179517 は 23 報の論文で使用されています。

    • Amadeu SO  et al. Arthrospira (Spirulina) platensis feeding reduces the early stage of chemically induced rat colon carcinogenesis. Br J Nutr 129:395-405 (2023). PubMed: 35506448
    • Xie ZQ  et al. Capsaicin suppresses hepatocarcinogenesis by inhibiting the stemness of hepatic progenitor cells via SIRT1/SOX2 signaling pathway. Cancer Med 11:4283-4296 (2022). PubMed: 35674129
    • Yang H  et al. CRISPR/Cas9‑induced saturated mutagenesis identifies Rad51 haplotype as a marker of PARP inhibitor sensitivity in breast cancer. Mol Med Rep 26:N/A (2022). PubMed: 35713220
    • Huo X  et al. Lentinan Enhances the Function of Oxaliplatin on the Esophageal Tumors by Persuading Immunogenic Cell Death. Comput Math Methods Med 2022:2296574 (2022). PubMed: 35844448
    • Zhu S  et al. Injectable conductive gelatin methacrylate / oxidized dextran hydrogel encapsulating umbilical cord mesenchymal stem cells for myocardial infarction treatment. Bioact Mater 13:119-134 (2022). PubMed: 35224296
    View all Publications for this product

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