Anti-Caspase-3 抗体 (ab4051)
Key features and details
- Rabbit polyclonal to Caspase-3
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Caspase-3 antibody
Caspase-3 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Caspase-3 -
由来種
Rabbit -
特異性
Caspase-3 is a member of the interleukin-1 β-converting enzyme family. Caspase-3 is thought to be associated with induction of apoptosis. Caspase-3 is synthesized as inactive 32 kDa proenzyme and is processed during apoptosis generating two subunits of 17 kDa and 12 kDa. Caspase-3 stains the epithelial cells of skin, renal proximal tubules and collecting ducts.
This antibody reacts with the 32 kDa proenzyme and the 17 kDa active form of caspase 3.
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アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human Caspase-3 aa 167-175. Corresponding to the cleavage site of human caspase 3.
Database link: P42574 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.3
Preservative: 0.05% Sodium azide
Constituent: 1% BSA -
Concentration information loading... -
精製度
IgG fraction -
特記事項（精製）
Purified immunoglobulin fraction of rabbit antiserum against Caspase-3 containing sodium azide as a preservative. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Assay kits
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab4051の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use at an assay dependent concentration. Predicted molecular weight: 31 kDa.
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| IHC-P | (6) |
Use a concentration of 0.002 mg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required. |
| 特記事項 |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 31 kDa. |
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IHC-P
Use a concentration of 0.002 mg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required. |
ターゲット情報
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機能
Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-
-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. -
組織特異性
Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system. -
配列類似性
Belongs to the peptidase C14A family. -
翻訳後修飾
Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa.
S-nitrosylated on its catalytic site cysteine in unstimulated human cell lines and denitrosylated upon activation of the Fas apoptotic pathway, associated with an increase in intracellular caspase activity. Fas therefore activates caspase-3 not only by inducing the cleavage of the caspase zymogen to its active subunits, but also by stimulating the denitrosylation of its active site thiol. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 836 Human
- Omim: 600636 Human
- SwissProt: P42574 Human
- Unigene: 141125 Human
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別名
- A830040C14Rik antibody
- Apopain antibody
- CASP 3 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-3 antibody (ab4051)This image is courtesy of an anonymous Abreview.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of formaldehyde-fixed human brain cortex tissue staining with ab4051 at 1/200 dilution. Secondary antibody was HRP Agilent anti-mouse/rabbit EnVision Dual Link. Samples were incubated with the primary antibody with Agilent antibody diluent for 14 hours at 4°C. Blocking was done using 3% serum for 1 hour at 22°C. Heat mediated antigen retrieval with pH = 6 citrate.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-3 antibody (ab4051)ab4051 staining Caspase-3 in formalin-fixed, paraffin-embedded Human tonsil tissue by Immunohistochemistry.
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Western blot - Anti-Caspase-3 antibody (ab4051)Anti-Caspase-3 antibody (ab4051) + Human Lung Cell Extract
Predicted band size: 31 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-3 antibody (ab4051)Image courtesy of an anonymous AbReviewab4051 staining Caspase 3 in Pig liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 5% serum for 1 hour at 21°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/100 in milk) for 20 hours at 4°C. A Biotin-conjugated Goat anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-3 antibody (ab4051)Image courtesy of an anonymous AbReviewab4051 staining Caspase 3 in Cow Ovary tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 10% serum for 15 minutes at 25°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/75 in PBS + BSA) for 16 hours at 4°C. A Biotin-conjugated Goat anti-rabbit IgG polyclonal (1/100) was used as the secondary antibody.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (315)
ab4051 は 315 報の論文で使用されています。
- Tam JSY et al. Investigation of TLR4 Antagonists for Prevention of Intestinal Inflammation. Inflammation 46:103-114 (2023). PubMed: 35867263
- Azouz RA et al. Silica Nanoparticle-Induced Reproductive Toxicity in Male Albino Rats via Testicular Apoptosis and Oxidative Stress. Biol Trace Elem Res 201:1816-1824 (2023). PubMed: 35672543
- Elseady WS et al. Saffron extract attenuates Sofosbuvir-induced retinal neurodegeneration in albino rat. Anat Rec (Hoboken) 306:422-436 (2023). PubMed: 35451203
- Bolat N et al. Selenium ameliorates inflammation by decreasing autophagic flux and mitogen-activated protein kinase signalling on experimentally induced rat periapical lesions. Int Endod J 56:227-244 (2023). PubMed: 36314140
- Hashemi M et al. The effect of hydroalcoholic extract of Cichorium intybus leaf on aryl hydrocarbon receptor expression in the testis of Wistar rats exposed to cigarette smoke. Avicenna J Phytomed 13:58-69 (2023). PubMed: 36698732