Anti-Caspase-2 抗体 [Y154] (ab32021)
![Western blot - Anti-Caspase-2 antibody [Y154] (ab32021)](https://www.abcam.co.jp/ps/products/32/ab32021/Images/ab32021-253275-anti-caspase-2-antibody-y154-western-blot.jpg "Western blot - Anti-Caspase-2 antibody [Y154] (ab32021)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [Y154] to Caspase-2
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Caspase-2 antibody [Y154]
Caspase-2 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [Y154] to Caspase-2 -
由来種
Rabbit -
特異性
This antibody is specific for the pro form and the p18 cleaved form of Caspase-2. -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide within Human Caspase-2 aa 250-350 (C terminal). The exact sequence is proprietary.
Database link: P42575 -
ポジティブ･コントロール
- Jurkat cell lysate.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
Y154 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab32021の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500. Detects a band of approximately 50 kDa (predicted molecular weight: 49 kDa).
Detects bands of approximately 50 kDa (pro form) and 18 kDa (p18 cleaved form). |
| 特記事項 |
|---|
|
WB
1/500. Detects a band of approximately 50 kDa (predicted molecular weight: 49 kDa). Detects bands of approximately 50 kDa (pro form) and 18 kDa (p18 cleaved form). |
ターゲット情報
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機能
Involved in the activation cascade of caspases responsible for apoptosis execution. Might function by either activating some proteins required for cell death or inactivating proteins necessary for cell survival. -
組織特異性
Expressed at higher levels in the embryonic lung, liver and kidney than in the heart and brain. In adults, higher level expression is seen in the placenta, lung, kidney, and pancreas than in the heart, brain, liver and skeletal muscle. -
配列類似性
Belongs to the peptidase C14A family.
Contains 1 CARD domain. -
翻訳後修飾
The mature protease can process its own propeptide, but not that of other caspases. - Information by UniProt
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参照データベース
- Entrez Gene: 835 Human
- Omim: 600639 Human
- SwissProt: P42575 Human
- Unigene: 368982 Human
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別名
- CASP 2 antibody
- CASP-2 antibody
- Casp2 antibody
see all
画像
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Western blot - Anti-Caspase-2 antibody [Y154] (ab32021)
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: Caspase 2 knockout HAP1 cell lysate (20 µg)Lanes 1 and 2: Merged signal (red and green). Green - ab182738 observed at 51 kDa. Red - loading control, ab8226, observed at 42 kDa.
ab32021 was shown to recognize caspase 2 when caspase 2 knockout samples were used, along with additional cross-reactive bands. Wild-type and caspase 2 knockout samples were subjected to SDS-PAGE. ab32021 and ab8226 (loading control to beta actin) were diluted 1/50 and 1/1000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging. -
![Western blot - Anti-Caspase-2 antibody [Y154] (ab32021)](https://www.abcam.co.jp/ps/products/32/ab32021/Images/ab32021-43662-anti-caspase-2-antibody-y154-western-blot.jpg)
Western blot - Anti-Caspase-2 antibody [Y154] (ab32021)Anti-Caspase-2 antibody [Y154] (ab32021) at 1/500 dilution + Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Predicted band size: 49 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Additional bands at: 18 kDa (possible cleavage fragment) -
![Anti-Caspase-2 antibody [Y154] (ab32021)](https://www.abcam.co.jp/ps/products/32/ab32021/Images/ab32021-3-benefits-of-recombinant-antibodies.png)
Anti-Caspase-2 antibody [Y154] (ab32021)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (10)
ab32021 は 10 報の論文で使用されています。
- Jiang L & Li J lncRNA GMDS-AS1 upregulates IL-6, TNF-a and IL-1ß, and induces apoptosis in human monocytic THP-1 cells via miR-96-5p/caspase 2 signaling. Mol Med Rep 25:N/A (2022). PubMed: 34981821
- Šrámek J et al. Calcium channel blockers do not protect against saturated fatty acid-induced ER stress and apoptosis in human pancreatic ß-cells. Nutr Metab (Lond) 18:74 (2021). PubMed: 34274001
- Mizoguchi A et al. MicroRNA-8073: Tumor suppressor and potential therapeutic treatment. PLoS One 13:e0209750 (2018). PubMed: 30589909
- Sikorski K et al. A high-throughput pipeline for validation of antibodies. Nat Methods 15:909-912 (2018). PubMed: 30377371
- Majumder P et al. Cellular levels of Grb2 and cytoskeleton stability are correlated in a neurodegenerative scenario. Dis Model Mech 10:655-669 (2017). PubMed: 28360125
- Yang GW et al. Ferulic Acid Exerts Anti-Angiogenic and Anti-Tumor Activity by Targeting Fibroblast Growth Factor Receptor 1-Mediated Angiogenesis. Int J Mol Sci 16:24011-31 (2015). PubMed: 26473837
- Shen Q et al. Regulation of CRADD-caspase 2 cascade by histone deacetylase 1 in gastric cancer. Am J Transl Res 6:538-47 (2014). PubMed: 25360218
- Jelínek M et al. Caspase-2 is involved in cell death induction by taxanes in breast cancer cells. Cancer Cell Int 13:42 (2013). WB . PubMed: 23672670
- Yoon JY et al. Synergistic apoptotic response between valproic acid and fludarabine in chronic lymphocytic leukaemia (CLL) cells involves the lysosomal protease cathepsin B. Blood Cancer J 3:e153 (2013). Human, Human, Human, Human . PubMed: 24141622
- Aiyar SE et al. TMS, a chemically modified herbal derivative of resveratrol, induces cell death by targeting Bax. Breast Cancer Res Treat 124:265-77 (2010). PubMed: 20490654