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リコンビナントRabMAb

Anti-Caspase-10/CASP-10 抗体 [EPR10890] (ab177475)

Reviews (1) Submit a question References (3)
Western blot - Anti-Caspase-10/CASP-10 antibody [EPR10890] (ab177475)
  • Immunoprecipitation - Anti-Caspase-10/CASP-10 antibody [EPR10890] (ab177475)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-10/CASP-10 antibody [EPR10890] (ab177475)
  • Flow Cytometry (Intracellular) - Anti-Caspase-10/CASP-10 antibody [EPR10890] (ab177475)
  • Anti-Caspase-10/CASP-10 antibody [EPR10890] (ab177475)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10890] to Caspase-10/CASP-10
  • Suitable for: Flow Cyt (Intra), WB, IHC-P, IP
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-Caspase-10/CASP-10 antibody [EPR10890]
    Caspase-10/CASP-10 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR10890] to Caspase-10/CASP-10

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Flow Cyt (Intra), WB, IHC-P, IPmore details
    適用なし: ICC/IF

  • 種交差性

    交差種: Human

  • 免疫原

    Recombinant fragment within Human Caspase-10/CASP-10. The exact sequence is proprietary.
    Database link: Q92851

  • ポジティブ･コントロール

    • K562 and Jurkat whole cell lysate (ab7899); Human hepatocellular carcinoma tissue; K562 cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab177475の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB (1)
1/1000 - 1/10000. Predicted molecular weight: 58 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP
1/10 - 1/100.
特記事項
Flow Cyt (Intra)
1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000 - 1/10000. Predicted molecular weight: 58 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP
1/10 - 1/100.
追加情報
Is unsuitable for ICC/IF.

ターゲット情報

  • 機能

    Involved in the activation cascade of caspases responsible for apoptosis execution. Recruited to both Fas- and TNFR-1 receptors in a FADD dependent manner. May participate in the granzyme B apoptotic pathways. Cleaves and activates caspase-3, -4, -6, -7, -8, and -9. Hydrolyzes the small- molecule substrates, Tyr-Val-Ala-Asp-
    -AMC and Asp-Glu-Val-Asp-
    -AMC.
    Isoform C is proteolytically inactive.

  • 組織特異性

    Detectable in most tissues. Lowest expression is seen in brain, kidney, prostate, testis and colon.

  • 関連疾患

    Defects in CASP10 are the cause of autoimmune lymphoproliferative syndrome type 2A (ALPS2A) [MIM:603909]. ALPS2 is characterized by abnormal lymphocyte and dendritic cell homeostasis and immune regulatory defects.
    Defects in CASP10 are a cause of familial non-Hodgkin lymphoma (NHL) [MIM:605027]. NHL is a cancer that starts in cells of the lymph system, which is part of the body's immune system. NHLs can occur at any age and are often marked by enlarged lymph nodes, fever and weight loss.
    Defects in CASP10 are a cause of gastric cancer (GASC) [MIM:613659]. A malignant disease which starts in the stomach, can spread to the esophagus or the small intestine, and can extend through the stomach wall to nearby lymph nodes and organs. It also can metastasize to other parts of the body. The term gastric cancer or gastric carcinoma refers to adenocarcinoma of the stomach that accounts for most of all gastric malignant tumors. Two main histologic types are recognized, diffuse type and intestinal type carcinomas. Diffuse tumors are poorly differentiated infiltrating lesions resulting in thickening of the stomach. In contrast, intestinal tumors are usually exophytic, often ulcerating, and associated with intestinal metaplasia of the stomach, most often observed in sporadic disease.

  • 配列類似性

    Belongs to the peptidase C14A family.
    Contains 2 DED (death effector) domains.

  • 翻訳後修飾

    Cleavage by granzyme B and autocatalytic activity generate the two active subunits.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Information by UniProt

  • 参照データベース

    • 別名

      • ALPS2 antibody
      • Apoptotic protease Mch-4 antibody
      • CASP 10 antibody
      • CASP-10 antibody
      • CASP10 antibody
      • CASPA_HUMAN antibody
      • Caspase 10 apoptosis related cysteine peptidase antibody
      • Caspase-10 antibody
      • Caspase-10 subunit p12 antibody
      • FADD like ICE2 antibody
      • Fas associated death domain protein antibody
      • FAS-associated death domain protein interleukin-1B-converting enzyme 2 antibody
      • FLICE 2 antibody
      • FLICE2 antibody
      • ICE like apoptotic protease 4 antibody
      • ICE-like apoptotic protease 4 antibody
      • Interleukin 1B converting enzyme 2 antibody
      • MCH 4 antibody
      see all

    画像

    • Western blot - Anti-Caspase-10/CASP-10 antibody [EPR10890] (ab177475)
      Western blot - Anti-Caspase-10/CASP-10 antibody [EPR10890] (ab177475)
      All lanes : Anti-Caspase-10/CASP-10 antibody [EPR10890] (ab177475) at 1/1000 dilution

      Lane 1 : K562 cell lysate
      Lane 2 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 58 kDa

    • Immunoprecipitation - Anti-Caspase-10/CASP-10 antibody [EPR10890] (ab177475)
      Immunoprecipitation - Anti-Caspase-10/CASP-10 antibody [EPR10890] (ab177475)

      Western blot analysis on immunoprecipitation pellet from (1) K562 cell lysate or (2) 1XPBS (negative control) using ab177475 and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-10/CASP-10 antibody [EPR10890] (ab177475)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-10/CASP-10 antibody [EPR10890] (ab177475)

      Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling Caspase-10/CASP-10 with ab177475 at 1/50 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Flow Cytometry (Intracellular) - Anti-Caspase-10/CASP-10 antibody [EPR10890] (ab177475)
      Flow Cytometry (Intracellular) - Anti-Caspase-10/CASP-10 antibody [EPR10890] (ab177475)

      Intracellular flow cytometric analysis of permeabilized K562 cells labeling Caspase 10/CASP-10 with ab177475 at 1/100 dilution (red) compared toa rabbit IgG negative control (green). 

       

       

    • Anti-Caspase-10/CASP-10 antibody [EPR10890] (ab177475)
      Anti-Caspase-10/CASP-10 antibody [EPR10890] (ab177475)

    参考文献 (3)

    ab177475 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab177475 は 3 報の論文で使用されています。

    • Yu CC  et al. YWHAZ amplification/overexpression defines aggressive bladder cancer and contributes to chemo-/radio-resistance by suppressing caspase-mediated apoptosis. J Pathol N/A:N/A (2019). PubMed: 30945298
    • Xiao F  et al. Sex-dependent aortic valve pathology in patients with rheumatic heart disease. PLoS One 12:e0180230 (2017). IHC-P ; Human . PubMed: 28662157
    • Guo W  et al. Role of caspase-10 in the death of acute leukemia cells. Oncol Lett 12:1623-1629 (2016). WB ; Human . PubMed: 27446483

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    Western blot abreview for Anti-Caspase-10/CASP-10 antibody [EPR10890]

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (MOLT4)
    Gel Running Conditions
    Reduced Denaturing (4-20% gradient)
    Loading amount
    100000 cells
    Treatment
    Bortezomib 0-20hours
    Specification
    MOLT4
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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    Verified customer

    投稿 Jun 19 2015

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