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AB177475

Anti-Caspase-10/CASP-10 抗体 [EPR10890]

Anti-Caspase-10/CASP-10 antibody [EPR10890]

4

(1 Review)

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(6 Publications)

Rabbit Recombinant Monoclonal Caspase-10/CASP-10 antibody. Suitable for IHC-P, IP, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 6 publications.

別名を表示する

MCH4, CASP10, Caspase-10, CASP-10, Apoptotic protease Mch-4, FAS-associated death domain protein interleukin-1B-converting enzyme 2, ICE-like apoptotic protease 4, FLICE2

5 Images
Flow Cytometry (Intracellular) - Anti-Caspase-10/CASP-10 antibody [EPR10890] (AB177475)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Caspase-10/CASP-10 antibody [EPR10890] (AB177475)

Intracellular flow cytometric analysis of permeabilized K562 cells labeling Caspase 10/CASP-10 with ab177475 at 1/100 dilution (red) compared toa rabbit IgG negative control (green).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-10/CASP-10 antibody [EPR10890] (AB177475)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-10/CASP-10 antibody [EPR10890] (AB177475)

Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling Caspase-10/CASP-10 with ab177475 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunoprecipitation - Anti-Caspase-10/CASP-10 antibody [EPR10890] (AB177475)
  • IP

Supplier Data

Immunoprecipitation - Anti-Caspase-10/CASP-10 antibody [EPR10890] (AB177475)

Western blot analysis on immunoprecipitation pellet from (1) K562 cell lysate or (2) 1XPBS (negative control) using ab177475 and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

All lanes:

Immunoprecipitation - Anti-Caspase-10/CASP-10 antibody [EPR10890] (ab177475)

Predicted band size: 58 kDa

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Western blot - Anti-Caspase-10/CASP-10 antibody [EPR10890] (AB177475)
  • WB

Supplier Data

Western blot - Anti-Caspase-10/CASP-10 antibody [EPR10890] (AB177475)

All lanes:

Western blot - Anti-Caspase-10/CASP-10 antibody [EPR10890] (ab177475) at 1/1000 dilution

Lane 1:

K562 cell lysate at 10 µg

Lane 2:

Jurkat cell lysate at 10 µg

Predicted band size: 58 kDa

false

Western blot - Anti-Caspase-10/CASP-10 antibody [EPR10890] (AB177475)
  • WB

Supplier Data

Western blot - Anti-Caspase-10/CASP-10 antibody [EPR10890] (AB177475)

Blocking and diluting buffer : 5% NFDM /TBST.

Exposure time :

Lane 1 & 2 : 7 seconds

Lane 3 & 4 : 3 seconds

Lanes 1 - 2:

Western blot - Anti-Pro Caspase-8 antibody [EPR162] (<a href='/products/primary-antibodies/pro-caspase-8-antibody-epr162-ab108333'>ab108333</a>) at 1/1000 dilution

Lanes 3 - 4:

Western blot - Anti-Caspase-10/CASP-10 antibody [EPR10890] (ab177475) at 1/1000 dilution

Lanes 1 - 2:

His-tagged Human full-length Caspase 8 recombinant protein at 10 ng

Lanes 3 - 4:

His-tagged Human full-length Caspase 10 recombinant protein at 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 60-70 kDa

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関連する標識済み抗体及び組成の異なる製品 (1)

  • Carrier free

    Anti-Caspase-10/CASP-10 antibody [EPR10890] - BSA and Azide free

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR10890

アイソタイプ

IgG

キャリアフリー

No

交差種

Human

アプリケーション

Flow Cyt (Intra), IP, WB, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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代替製品

Primary Antibodies

AB2012

Anti-Caspase-10/CASP-10 antibody

Immunocytochemistry - Anti-Caspase-10/CASP-10 antibody (AB2012)

  • 5
  • 1

組合せ製品

Assay Kits

AB219937

Caspase-10 (active) Staining Kit - Green Fluorescence

Functional Studies - Caspase-10 (active) Staining Kit - Green Fluorescence (AB219937)

  • 0
  • 0
Primary Antibodies

AB184787

Anti-Caspase-3 antibody [EPR18297]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-3 antibody [EPR18297] (AB184787)

  • 3
  • 3
Primary Antibodies

AB215191

Anti-DFNA5/GSDME antibody [EPR19859] - N-terminal

20ul selling size|RabMAb|Recombinant|KO Validated

Western blot - Anti-DFNA5/GSDME antibody [EPR19859] - N-terminal (AB215191)

  • 5
  • 4
Primary Antibodies

AB108601

Anti-FADD antibody [EPR4415]

RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-FADD antibody [EPR4415] (AB108601)

  • 0
  • 0
Primary Antibodies

AB215203

Anti-cleaved N-terminal GSDMD antibody [EPR20829-408]

RabMAb|Recombinant|20ul selling size

Western blot - Anti-cleaved N-terminal GSDMD antibody [EPR20829-408] (AB215203)

  • 5
  • 4
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100 - 1/500", "FlowCytIntra-species-notes": "<p><a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" } } }
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製品の詳細

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
出荷温度
Conditional Ambient
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Involved in the activation cascade of caspases responsible for apoptosis execution. Recruited to both Fas- and TNFR-1 receptors in a FADD dependent manner. May participate in the granzyme B apoptotic pathways. Cleaves and activates effector caspases CASP3, CASP4, CASP6, CASP7, CASP8 and CASP9. Hydrolyzes the small- molecule substrates, Tyr-Val-Ala-Asp-|-AMC and Asp-Glu-Val-Asp-|-AMC.. Isoform 7 can enhance NF-kappaB activity but promotes only slight apoptosis.. Isoform C is proteolytically inactive.
See full target information CASP10
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文献 (6)

Recent publications for all applications. Explore the full list and refine your search

Archives of toxicology 98:1859-1875 PubMed38555327

2024

Effects of the nerve agent VX on hiPSC-derived motor neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Catherine Schaefers,Wolfgang Schmeißer,Harald John,Franz Worek,Theo Rein,Simone Rothmiller,Annette Schmidt

Nature communications 14:3742 PubMed37353488

2023

Systems-level analyses of protein-protein interaction network dysfunctions via epichaperomics identify cancer-specific mechanisms of stress adaptation.

Applications

Unspecified application

Species

Unspecified reactive species

Anna Rodina,Chao Xu,Chander S Digwal,Suhasini Joshi,Yogita Patel,Anand R Santhaseela,Sadik Bay,Swathi Merugu,Aftab Alam,Pengrong Yan,Chenghua Yang,Tanaya Roychowdhury,Palak Panchal,Liza Shrestha,Yanlong Kang,Sahil Sharma,Justina Almodovar,Adriana Corben,Mary L Alpaugh,Shanu Modi,Monica L Guzman,Teng Fei,Tony Taldone,Stephen D Ginsberg,Hediye Erdjument-Bromage,Thomas A Neubert,Katia Manova-Todorova,Meng-Fu Bryan Tsou,Jason C Young,Tai Wang,Gabriela Chiosis

Cell biology international 45:154-163 PubMed33049089

2020

Long noncoding RNA PVT1 facilitates high glucose-induced cardiomyocyte death through the miR-23a-3p/CASP10 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Feng-Rong Yu,Yin-Wen Xia,Shao-Bo Wang,Li-Hua Xiao

The Journal of pathology 248:476-487 PubMed30945298

2019

YWHAZ amplification/overexpression defines aggressive bladder cancer and contributes to chemo-/radio-resistance by suppressing caspase-mediated apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Chia-Cheng Yu,Chien-Feng Li,I-Hsuan Chen,Ming-Tsung Lai,Zi-Jun Lin,Praveen K Korla,Chee-Yin Chai,Grace Ko,Chih-Mei Chen,Tritium Hwang,Shan-Chih Lee,Jim J-C Sheu

PloS one 12:e0180230 PubMed28662157

2017

Sex-dependent aortic valve pathology in patients with rheumatic heart disease.

Applications

IHC-P

Species

Human

Feng Xiao,Rui Zheng,Di Yang,Kejiang Cao,Shijiang Zhang,Bingruo Wu,Yongfeng Shao,Bin Zhou

Oncology letters 12:1623-1629 PubMed27446483

2016

Role of caspase-10 in the death of acute leukemia cells.

Applications

WB

Species

Human

Wenjian Guo,Aishu Dong,Xiahui Pan,Xiaoji Lin,Ying Lin,Muqing He,Baoling Zhu,Liming Jin,Rongxing Yao
View all publications
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Abcam product promise

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