Anti-Casein Kinase 1 gamma 2 抗体 (ab64829)
Key features and details
- Rabbit polyclonal to Casein Kinase 1 gamma 2
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Casein Kinase 1 gamma 2 antibody
Casein Kinase 1 gamma 2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Casein Kinase 1 gamma 2 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
Synthetic peptide derived from N-terminus of human Casein Kinase 1 gamma 2.
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ポジティブ･コントロール
- HeLa and 293 cell extracts.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab64829の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
1/500 - 1/1000. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).
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| 特記事項 |
|---|
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WB
1/500 - 1/1000. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa). |
ターゲット情報
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機能
Serine/threonine-protein kinase. Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling (By similarity). Phosphorylates COL4A3BP/CERT, MTA1 and SMAD3. Involved in brain development and vesicular trafficking and neurotransmitter releasing from small synaptic vesicles. Regulates fast synaptic transmission mediated by glutamate. SMAD3 phosphorylation promotes its ligand-dependent ubiquitination and subsequent proteasome degradation, thus inhibiting SMAD3-mediated TGF-beta responses. Hyperphosphorylation of the serine-repeat motif of COL4A3BP/CERT leads to its inactivation by dissociation from the Golgi complex, thus down-regulating ER-to-Golgi transport of ceramide and sphingomyelin synthesis. -
組織特異性
Testis. -
配列類似性
Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.
Contains 1 protein kinase domain. -
翻訳後修飾
Autophosphorylated. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 1455 Human
- Entrez Gene: 103236 Mouse
- Entrez Gene: 65278 Rat
- Omim: 602214 Human
- SwissProt: P78368 Human
- SwissProt: Q8BVP5 Mouse
- SwissProt: Q62762 Rat
- Unigene: 651905 Human
see all -
別名
- Casein kinase 1, gamma 2 antibody
- Casein kinase I isoform gamma 2 antibody
- Casein kinase I isoform gamma-2 antibody
see all
画像
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Western blot - Anti-Casein Kinase 1 gamma 2 antibody (ab64829)This image is courtesy of an anonymous AbreviewLanes 1-2 : Anti-Casein Kinase 1 gamma 2 antibody (ab102554)
Lanes 3-4 : Anti-Casein Kinase 1 gamma 2 antibody (ab70862)
Lanes 5-6 : Anti-Casein Kinase 1 gamma 2 antibody (ab64829) at 1/500 dilution
Lanes 1 & 3 & 5 : U2OS cells expressing FLAG-tagged CK1G2 plasmid
Lane 2 : U2OS cells expressing FLAG tag only plasmid
Lanes 4 & 6 : U2OS cells expressing FLAG only
Secondary
Lanes 5-6 : HRP-conjugated Goat anti-rabbit polyclonal at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 47 kDa
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Western blot - Anti-Casein Kinase 1 gamma 2 antibody (ab64829)All lanes : Anti-Casein Kinase 1 gamma 2 antibody (ab64829) at 1/500 dilution
Lane 1 : HeLa cell extract
Lane 2 : 293 cell extract
Lane 3 : 293 cell extract with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 47 kDa
Observed band size: 47 kDa
Additional bands at: 25 kDa, 34 kDa. We are unsure as to the identity of these extra bands.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (6)
ab64829 は 6 報の論文で使用されています。
- Agajanian MJ et al. Protein proximity networks and functional evaluation of the casein kinase 1 gamma family reveal unique roles for CK1γ3 in WNT signaling. J Biol Chem 298:101986 (2022). PubMed: 35487243
- Shen S et al. Induction of Huh-7 cell apoptosis by HCV core proteins via CK1a-p53-Bid signaling pathway. Mol Med Rep 17:7559-7566 (2018). PubMed: 29620268
- Vinyoles M et al. Activation of CK1? by PP2A/PR61? is required for the initiation of Wnt signaling. Oncogene 36:429-438 (2017). Human . PubMed: 27321178
- Bustos V et al. Phosphorylated Presenilin 1 decreases ß-amyloid by facilitating autophagosome-lysosome fusion. Proc Natl Acad Sci U S A 114:7148-7153 (2017). WB ; Mouse . PubMed: 28533369
- Del Valle-Pérez B et al. Coordinated Action of CK1 Isoforms in Canonical Wnt Signaling. Mol Cell Biol 31:2877-88 (2011). WB ; Human . PubMed: 21606194
- Casagolda D et al. A p120-catenin-CK1epsilon complex regulates Wnt signaling. J Cell Sci 123:2621-31 (2010). WB ; Human . PubMed: 20940130