Anti-Casein Kinase 1 delta/CSNK1D 抗体 (ab48031)
Key features and details
- Goat polyclonal to Casein Kinase 1 delta/CSNK1D
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
-
製品名
Anti-Casein Kinase 1 delta/CSNK1D antibody
Casein Kinase 1 delta/CSNK1D 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to Casein Kinase 1 delta/CSNK1D -
由来種
Goat -
特異性
This antibody is expected to recognise both reported isoforms of Casein Kinase 1 delta/CSNK1D.
-
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Cow, Dog, Chimpanzee, Macaque monkey
-
免疫原
Synthetic peptide corresponding to Human Casein Kinase 1 delta/CSNK1D aa 310-324 (internal sequence).
Sequence:DREERLRHSRNPATR
Database link: P48730 -
ポジティブ･コントロール
- WB: HEK 293 cell lysate. ICC/UF U251 cells.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab48031の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 47 kDa).
1 hour primary incubation is recommended for this product. |
| ICC/IF |
Use a concentration of 10 µg/ml.
|
| 特記事項 |
|---|
|
WB
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 47 kDa). 1 hour primary incubation is recommended for this product. |
|
ICC/IF
Use a concentration of 10 µg/ml. |
ターゲット情報
-
機能
Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Central component of the circadian clock. May act as a negative regulator of circadian rhythmicity by phosphorylating PER1 and PER2. Retains PER1 in the cytoplasm. -
組織特異性
Expressed in all tissues examined, including brain, heart, lung, liver, pancreas, kidney, placenta and skeletal muscle. In blood, highly expressed in hemopoietic cells and mature granulocytes. Also found in monocytes and lymphocytes. -
関連疾患
Defects in CSNK1D are a cause of familial advanced sleep-phase syndrome (FASPS) [MIM:604348]. FASPS is characterized by very early sleep onset and offset. Individuals are 'morning larks' with a 4 hours advance of the sleep, temperature and melatonin rhythms. -
配列類似性
Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.
Contains 1 protein kinase domain. -
翻訳後修飾
Autophosphorylated on serine and threonine residues. -
細胞内局在
Cytoplasm. Nucleus. - Information by UniProt
-
参照データベース
- Entrez Gene: 407122 Cow
- Entrez Gene: 1453 Human
- Entrez Gene: 104318 Mouse
- Entrez Gene: 64462 Rat
- Omim: 600864 Human
- SwissProt: P35508 Cow
- SwissProt: P48730 Human
- SwissProt: Q9DC28 Mouse
see all -
別名
- Casein Kinase 1 delta antibody
- Casein kinase I delta isoform antibody
- Casein kinase I isoform delta antibody
see all
画像
-
Western blot - Anti-Casein Kinase 1 delta/CSNK1D antibody (ab48031)Anti-Casein Kinase 1 delta/CSNK1D antibody (ab48031) at 0.3 µg/ml + HEK 293 cell lysate in RIPA buffer at 35 µg
Predicted band size: 47 kDa
Observed band size: 48 kDa why is the actual band size different from the predicted?Primary incubation was 1 hour. Detected using chemiluminescence.
-
Immunocytochemistry/ Immunofluorescence - Anti-Casein Kinase 1 delta/CSNK1D antibody (ab48031)Immunocytochemistry/Immunofluorescence analysis of U251 cells labelling Casein Kinase 1 delta/CSNK1D with ab48031 at 10 µg/ml. Cells were paraformaldehyde fixed and permeabilized with 0.15% triton. Primary antibody icubation was for 1 hour followed by incubation with an Alexa Fluor® 488-conjugated secondary antibody at 2 µg/ml. DAPI nuclear counterstain was used.
Negative control: Unimmunized goat IgG (10 µg/ml) followed by an Alexa Fluor® 488-conjugated secondary antibody (2 µg/ml).
データシートおよび資料
-
Datasheet download
参考文献 (4)
ab48031 は 4 報の論文で使用されています。
- Hotta T et al. EML2-S constitutes a new class of proteins that recognizes and regulates the dynamics of tyrosinated microtubules. Curr Biol 32:3898-3910.e14 (2022). PubMed: 35963242
- Vena F et al. Targeting Casein Kinase 1 Delta Sensitizes Pancreatic and Bladder Cancer Cells to Gemcitabine Treatment by Upregulating Deoxycytidine Kinase. Mol Cancer Ther 19:1623-1635 (2020). PubMed: 32430484
- Zhou A et al. Nuclear GSK3ß promotes tumorigenesis by phosphorylating KDM1A and inducing its deubiquitylation by USP22. Nat Cell Biol 18:954-966 (2016). PubMed: 27501329
- Zyss D et al. Casein kinase I delta controls centrosome positioning during T cell activation. J Cell Biol 195:781-97 (2011). PubMed: 22123863
