Anti-Casein Kinase 1 alpha (phospho T321) 抗体 (ab63559)
Key features and details
- Rabbit polyclonal to Casein Kinase 1 alpha (phospho T321)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Casein Kinase 1 alpha (phospho T321) antibody
Casein Kinase 1 alpha 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Casein Kinase 1 alpha (phospho T321) -
由来種
Rabbit -
特異性
Detects Casein Kinase I alpha only when phosphorylated at threonine 321. -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
Synthetic peptide corresponding to Human Casein Kinase 1 alpha aa 319-323 (phospho T321).
Sequence:A-Q-Tp-P-T
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ポジティブ･コントロール
- HuvEc cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab63559の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/1000. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).
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| 特記事項 |
|---|
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WB
1/500 - 1/1000. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa). |
ターゲット情報
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機能
Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates CTNNB1 at 'Ser-45'. May play a role in segregating chromosomes during mitosis. -
配列類似性
Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.
Contains 1 protein kinase domain. -
細胞内局在
Cytoplasm. Cytoplasm > cytoskeleton > centrosome. Chromosome > centromere > kinetochore. Nucleus speckle. Localizes to the centrosome in interphase cells, and to kinetochore fibers during mitosis. - Information by UniProt
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参照データベース
- Entrez Gene: 1452 Human
- Entrez Gene: 93687 Mouse
- Entrez Gene: 113927 Rat
- Omim: 600505 Human
- SwissProt: P48729 Human
- SwissProt: Q8BK63 Mouse
- SwissProt: P97633 Rat
- Unigene: 529862 Human
see all -
別名
- Casein kinase 1 alpha 1 antibody
- Casein kinase I isoform alpha antibody
- CK1 antibody
see all
画像
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Western blot - Anti-Casein Kinase 1 alpha (phospho T321) antibody (ab63559)All lanes : Anti-Casein Kinase 1 alpha (phospho T321) antibody (ab63559) at 1/500 dilution
Lane 1 : extracts from HuvEc cells
Lane 2 : extracts from HuvEc cells with immunizing phosphopeptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 39 kDa
Observed band size: 39 kDa
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab63559 は論文での使用が確認できていません。