Anti-Carma 1/CARD11 抗体 (ab115471)
Key features and details
- Goat polyclonal to Carma 1/CARD11
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Carma 1/CARD11 antibody
Carma 1/CARD11 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to Carma 1/CARD11 -
由来種
Goat -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
Synthetic peptide corresponding to Human Carma 1/CARD11 aa 1100-1200 (C terminal) (Cysteine residue).
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ポジティブ･コントロール
- Human spleen tissue; Jurkat cell lysate. The pattern of staining with this antibody is similar to that observed with ab189167 and ab113409 in that the reagent stains macrophages, mast cells, subsets of lymphocytes and plasma cells in various organs across the body. It also stains adrenal cortex, heart, kidney, small intestine epithelium, skeletal muscle, and pancreatic exocrine epithelium and Kupffer cells in the liver
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 99% Tris buffered saline -
Concentration information loading... -
精製度
Protein G purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab115471の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 133 kDa.
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| IHC-P |
Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 特記事項 |
|---|
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WB
Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 133 kDa. |
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IHC-P
Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Activates NF-kappa-B via BCL10 and IKK. Stimulates the phosphorylation of BCL10. -
組織特異性
Detected in adult peripheral blood leukocytes, thymus, spleen and liver. Also found in promyelocytic leukemia HL-60 cells, chronic myelogenous leukemia K562 cells, Burkitt's lymphoma Raji cells and colorectal adenocarcinoma SW480 cells. Not detected in HeLa S3, Molt-4, A549 and G431 cells. -
配列類似性
Contains 1 CARD domain.
Contains 1 guanylate kinase-like domain.
Contains 1 PDZ (DHR) domain. -
翻訳後修飾
Phosphorylation at Ser-559, Ser-644 and Ser-652 by PRKCBB leads to a shift from an inactive to an active form that activates the NF-kappa-B signaling. -
細胞内局在
Cytoplasm. Membrane raft. Colocalized with DPP4 in membrane rafts. - Information by UniProt
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参照データベース
- Entrez Gene: 84433 Human
- Entrez Gene: 108723 Mouse
- Entrez Gene: 304314 Rat
- Omim: 607210 Human
- SwissProt: Q9BXL7 Human
- SwissProt: Q8CIS0 Mouse
- Unigene: 648101 Human
- Unigene: 46187 Mouse
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別名
- 0610008L17Rik antibody
- 2410011D02Rik antibody
- Bcl10 interacting maguk protein 3 antibody
see all
画像
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Western blot - Anti-Carma 1/CARD11 antibody (ab115471)Anti-Carma 1/CARD11 antibody (ab115471) at 1 µg/ml + Jurkat cell lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 133 kDa
Primary incubation: 1 hour -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carma 1/CARD11 antibody (ab115471)ab115471 at 2ug/ml staining Carma/CARD11 in Formalin-fixed, Paraffin-embedded Human spleen tissue by Immunohistochemistry, followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab115471 は論文での使用が確認できていません。