Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online

Anti-Cardiotin 抗体 [R2G] (ab8962)

Submit a review Q&A (2)References (2)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiotin antibody [R2G] (ab8962)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiotin antibody [R2G] (ab8962)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiotin antibody [R2G] (ab8962)
  • Western blot - Anti-Cardiotin antibody [R2G] (ab8962)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiotin antibody [R2G] (ab8962)

Key features and details

  • Mouse monoclonal [R2G] to Cardiotin
  • Suitable for: IHC-P, WB
  • Reacts with: Human, Pig, Zebrafish
  • Isotype: IgM

製品の概要

  • 製品名

    Anti-Cardiotin antibody [R2G]

  • 製品の詳細

    Mouse monoclonal [R2G] to Cardiotin

  • 由来種

    Mouse

  • 特異性

    This antibody reacts with cardiotin, a mitochondrion-associated protein which is present in cardiomyocytes and skeletal muscle.

  • アプリケーション

    適用あり: IHC-P, WBmore details

  • 種交差性

    交差種: Human, Pig, Zebrafish

  • 免疫原

    Full length native protein (purified) corresponding to Chicken Cardiotin. Total protein extract of chicken gizzard

  • 特記事項

    Mitochondrial membrane proteins have been shown to be involved in several cellular processes such as the regulation of apoptosis and the energy metabolism. Cardiotin antibodies, which are reactive with mitochondrial proteins, have proven to be early indicators of ischemia induced changes in the myocardium.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab8962の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P
Use at an assay dependent concentration.
WB
1/25 - 1/500.
特記事項
IHC-P
Use at an assay dependent concentration.
WB
1/25 - 1/500.

ターゲット情報

  • 関連性

    Cardiotin is a high molecular weight protein complex (300 kDa) located in the mitochondrial membrane. The cardiotin structure exists as subunits of 60 kDa and 100 kDa. Both subunits contain the same amino-terminal 14 amino-acid sequence, showing high homology to human skeletal muscle a-actinin, suggesting that the tetrameric configuration of the cardiotin protein structure is a transmembrane complex with the N-terminus at the cytoplasmic side of the membrane, able to interact with actin. During cardiac contractile dysfunction, the cardiotin distribution is affected in pathlogical cardiomyocytes i.e. chronic ischemic myocardium. This monoclonal antibody can be used in immunohistochemistry for the detection of a disturbed mitochondrial activity in cardiomyocytes i.e. during chronic ischemia or chronic atrial fibrillation.

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiotin antibody [R2G] (ab8962)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiotin antibody [R2G] (ab8962)

    Indirect immunofluorescence analysis of swine heart cells labelling cardiotin with ab8962.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiotin antibody [R2G] (ab8962)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiotin antibody [R2G] (ab8962)

    Indirect immunofluorescence analysis of swine heart cells labelling cardiotin with ab8962.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiotin antibody [R2G] (ab8962)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiotin antibody [R2G] (ab8962)

    Human atrial cardiomyocytes stained with the monoclonal cardiotin antibody R2G.

  • Western blot - Anti-Cardiotin antibody [R2G] (ab8962)
    Western blot - Anti-Cardiotin antibody [R2G] (ab8962)

    Immunoblotting of the microsomal fraction of swine heart reveals the 100 kDa and 60 kDa cardiotin subunits after incubation with monoclonal antibodies (a) ab8963 clone SR-2, (b) ab8964 clone SR-3 and (c) ab8962 clone R2G.

    Immunoblotting of the microsomal fraction of swine heart reveals the 100 kDa and 60 kDa cardiotin subunits after incubation with monoclonal antibodies (a) ab8963 clone SR-2, (b) ab8964 clone SR-3 and (c) ab8962 clone R2G.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiotin antibody [R2G] (ab8962)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiotin antibody [R2G] (ab8962)
    Immunofluorescence staining of a 7 days old zebrafish embryo

参考文献 (2)

ab8962 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab8962 は 2 報の論文で使用されています。

  • Talavera-Adame D  et al. Enhancement of embryonic stem cell differentiation promoted by avian chorioallantoic membranes. Tissue Eng Part A 15:3193-200 (2009). PubMed: 19364272
  • Dispersyn GD  et al. Dissociation of cardiomyocyte apoptosis and dedifferentiation in infarct border zones. Eur Heart J 23:849-57 (2002). PubMed: 12042006

レビューと Q&A

Show All レビュー Q&A
レビューを送る 質問を送る

1-2 of 2 Abreviews or Q&A

Question

Please send a Certificate of Analysis for ab8962

Read More
Answer

Thank you very much for contacting us with your enquiry.

We are able to provide certificates of compliance instead of certificates of analysis, so please find the CoC of the current lot of ab8962 attached to this email.

If there is anything else that we can do for you, please let me know and I'll be happy to help you further.

Read More

Question

I am interested in your antibody with Cat#: Anti-Cardiotin antibody [R2G] (ab8962). Could you please send us the certificate of analysis of the current lot.

Read More
Answer

Thank you for your enquiry. I have attached a certificate of compliance for the lot currently in our inventory.

Some of our antibodies are produced in-house and others are sourced from suppliers or academic collaborators whose products we believe will be of interest to our customers. All these products are guaranteed by our Abpromise to be of high quality. Our Abpromise assures you that if our products do not perform as our datasheets say they should we offer a replacement or refund within 120 days of purchase. Some of our collaborators are not certified ISO9001 and so we are not able to provide a certificate of analysis for these particular products.

As the identity of our collaborators is confidential, we provide a certificate of compliance for all of our products available in our catalogue. This document certifies that our products meet the required specifications for a particular lot/batch of product.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com