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AB91605

Anti-Cardiac Troponin T 抗体 [EPR3695]

Anti-Cardiac Troponin T antibody [EPR3695]

5

(1 Review)

|

(16 Publications)

Anti-Cardiac Troponin T antibody [EPR3695] (ab91605) is a rabbit monoclonal antibody detecting Cardiac Troponin T in Western Blot, IP, IHC-P. Suitable for Human.

- Biophysical QC for unrivalled batch-batch consistency
- Over 10 publications
- Trusted since 2010

別名を表示する

TnTc, Cardiac muscle troponin T, cTnT, TNNT2

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin T antibody [EPR3695] (AB91605)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin T antibody [EPR3695] (AB91605)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cardiac muscle tissue sections labelling Cardiac Troponin T with purified ab91605 at 1/500 dilution (0.32 μg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used at 1/0 dilution. PBS instead of the primary antibody was used as the negative control. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Immunoprecipitation - Anti-Cardiac Troponin T antibody [EPR3695] (AB91605)
  • IP

Lab

Immunoprecipitation - Anti-Cardiac Troponin T antibody [EPR3695] (AB91605)

Purified ab91605 at 1/50 dilution (2μg) immunoprecipitating Cardiac Troponin T in Human heart lysate.
Lane 1 (input) : Human heart lysate 10μg
Lane 2 (+) : ab91605 + Human heart lysate.
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab91605 in Human heart lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration : 5% NFDM/TBST.
Diluting buffer and concentration : 5% NFDM/TBST.
Observed band size : 39 kDa

All lanes:

Immunoprecipitation - Anti-Cardiac Troponin T antibody [EPR3695] (ab91605)

Predicted band size: 35 kDa

false

Western blot - Anti-Cardiac Troponin T antibody [EPR3695] (AB91605)
  • WB

Lab

Western blot - Anti-Cardiac Troponin T antibody [EPR3695] (AB91605)

The molecular weight observed is consistent with what has been described in the literature (PMID : 15793653).

All lanes:

Western blot - Anti-Cardiac Troponin T antibody [EPR3695] (ab91605) at 1/1000 dilution

Lane 1:

Human heart lysate at 20 µg

Lane 2:

Human skeletal muscle lysate at 20 µg

Lane 3:

293T (Human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 35 kDa

Observed band size: 39 kDa

false

関連する標識済み抗体及び組成の異なる製品 (7)

  • Carrier free

    Anti-Cardiac Troponin T antibody [EPR3695] - BSA and Azide free

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-Cardiac Troponin T antibody [EPR3695]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-Cardiac Troponin T antibody [EPR3695]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Cardiac Troponin T antibody [EPR3695]

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Anti-Cardiac Troponin T antibody [EPR3695]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-Cardiac Troponin T antibody [EPR3695]

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-Cardiac Troponin T antibody [EPR3695]

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR3695

アイソタイプ

IgG

キャリアフリー

No

交差種

Human

アプリケーション

IHC-P, WB, IP

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "<p>The use of an HRP/AP polymerized secondary antibody is recommended as stronger signals have been found using these. IHC-P validated in Human samples only.</p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/50", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }
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下記製品にもご興味をお持ちいただけるかもしれません:

AB223860

Human Cardiac Troponin T ELISA Kit

0

0 Reviews

製品ページを見る
こちらの製品も推奨する理由

この製品は、同様の実験や関連する研究で広く使用されていることから推奨させていただいております。

お客様の実験に適するかご確認いただくため、必ず製品データシートをご確認いただくことをお勧めいたします。ご不明点等ございましたら、テクニカルサポートチームまでお問い合わせください。

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製品の詳細

What is this antibody validated in?
Anti-Cardiac Troponin T antibody [EPR3695] (ab91605) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC-P) in Human samples.

What is the molecular weight of Cardiac Troponin T?
Anti-Cardiac Troponin T [EPR3695] (ab91605) specifically detects a band for Cardiac Troponin T (UniProt: P45379) at a molecular weight of 36kDa.

Trusted by the scientific community
Anti-Cardiac Troponin T [EPR3695] (ab91605) was first used in a scientific publication in 2010 and has been cited over 10 times in peer-reviewed journals.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EPR3695] also available for your convenience: ab91605, Carrier free - ab247526, Alexa Fluor® 488 - ab311031, Alexa Fluor® 647 - ab311156, Alexa Fluor® 594 - ab311804, Alexa Fluor® 568 - ab313087, Alexa Fluor® 555 - ab313281, Alexa Fluor® 750 - ab321742

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
出荷温度
Conditional Ambient
短期保存温度
+4°C
長期保存温度
-20°C
保管に関する情報
Stable for 12 months at -20°C
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
See full target information TNNT2
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文献 (16)

Recent publications for all applications. Explore the full list and refine your search

Molecular therapy. Nucleic acids 34:102060 PubMed38028197

2023

Efficacy of exon-skipping therapy for DMD cardiomyopathy with mutations in actin binding domain 1.

Applications

Unspecified application

Species

Unspecified reactive species

Naoko Shiba,Xiao Yang,Mitsuto Sato,Shin Kadota,Yota Suzuki,Masahiro Agata,Kohei Nagamine,Masaki Izumi,Yusuke Honda,Tomoya Koganehira,Hideki Kobayashi,Hajime Ichimura,Shinichiro Chuma,Junichi Nakai,Shugo Tohyama,Keiichi Fukuda,Daigo Miyazaki,Akinori Nakamura,Yuji Shiba

NPJ Regenerative medicine 8:26 PubMed37236990

2023

Pluripotent stem cell-derived committed cardiac progenitors remuscularize damaged ischemic hearts and improve their function in pigs.

Applications

Unspecified application

Species

Unspecified reactive species

Lynn Yap,Li Yen Chong,Clarissa Tan,Swarnaseetha Adusumalli,Millie Seow,Jing Guo,Zuhua Cai,Sze Jie Loo,Eric Lim,Ru San Tan,Elina Grishina,Poh Loong Soong,Narayan Lath,Lei Ye,Enrico Petretto,Karl Tryggvason

Nucleic acids research 50:5313-5334 PubMed35544276

2022

Definition of germ layer cell lineage alternative splicing programs reveals a critical role for Quaking in specifying cardiac cell fate.

Applications

Unspecified application

Species

Unspecified reactive species

W Samuel Fagg,Naiyou Liu,Ulrich Braunschweig,Karen Larissa Pereira de Castro,Xiaoting Chen,Frederick S Ditmars,Steven G Widen,John Paul Donohue,Katalin Modis,William K Russell,Jeffrey H Fair,Matthew T Weirauch,Benjamin J Blencowe,Mariano A Garcia-Blanco

Frontiers in cardiovascular medicine 8:768873 PubMed34805322

2021

Cardiac Repair With Echocardiography-Guided Multiple Percutaneous Left Ventricular Intramyocardial Injection of hiPSC-CMs After Myocardial Infarction.

Applications

Unspecified application

Species

Unspecified reactive species

Xun Wu,Di Wang,Kele Qin,Chukwuemeka Daniel Iroegbu,Kun Xiang,Yuanjing Zhou,Qing Guan,Weijie Tang,Jun Peng,Jianjun Guo,Jinfu Yang,Chengming Fan

JCI insight 6: PubMed34676829

2021

TT-10-loaded nanoparticles promote cardiomyocyte proliferation and cardiac repair in a mouse model of myocardial infarction.

Applications

Unspecified application

Species

Unspecified reactive species

Wangping Chen,Danielle Pretorius,Yang Zhou,Yuji Nakada,Jinfu Yang,Jianyi Zhang

Stem cell research 53:102365 PubMed34087994

2021

Inhibition of EZH2 primes the cardiac gene activation via removal of epigenetic repression during human direct cardiac reprogramming.

Applications

Unspecified application

Species

Unspecified reactive species

Yawen Tang,Lianzhong Zhao,Xufen Yu,Jianyi Zhang,Li Qian,Jian Jin,Rui Lu,Yang Zhou

Oncology reports 44:628-636 PubMed32627044

2020

TNNT2 as a potential biomarker for the progression and prognosis of colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Li Jing,Li Feng,Zhiguo Zhou,Shuai Shi,Ruoying Deng,Zhicong Wang,Yi Zhang,Zhixue Ren,Yibing Liu

American journal of physiology. Heart and circulat 318:H801-H815 PubMed32057252

2020

DNA damage-free iPS cells exhibit potential to yield competent cardiomyocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Jessica M Miller,Nikhil M Mardhekar,Danielle Pretorius,Prasanna Krishnamurthy,Namakkal Soorappan Rajasekaran,Jianyi Zhang,Ramaswamy Kannappan

Journal of visualized experiments : JoVE : PubMed31355804

2019

Enhancing the Engraftment of Human Induced Pluripotent Stem Cell-derived Cardiomyocytes via a Transient Inhibition of Rho Kinase Activity.

Applications

Unspecified application

Species

Unspecified reactive species

Meng Zhao,Yawen Tang,Patrick J Ernst,Asher Kahn-Krell,Chengming Fan,Danielle Pretorius,Hanxi Zhu,Xi Lou,Lufang Zhou,Jianyi Zhang,Wuqiang Zhu

Cells, tissues, organs 206:82-94 PubMed30840966

2019

Hemodynamic Stimulation Using the Biomimetic Cardiac Tissue Model (BCTM) Enhances Maturation of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Aaron J Rogers,Ramaswamy Kannappan,Hadil Abukhalifeh,Mohammed Ghazal,Jessica M Miller,Ayman El-Baz,Vladimir G Fast,Palaniappan Sethu
View all publications
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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
保証に関する詳細については利用規約をご確認ください。
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