Anti-Cardiac Troponin T 抗体 [1C11] (ab8295)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin T antibody [1C11] (ab8295)](https://www.abcam.co.jp/ps/products/8/ab8295/Images/ab8295-318645-anti-cardiac-troponin-t-antibody-1c11-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin T antibody [1C11] (ab8295)")
Key features and details
- Mouse monoclonal [1C11] to Cardiac Troponin T
- Suitable for: ICC/IF, IHC-P, Sandwich ELISA
- Reacts with: Mouse, Rat, Dog, Human
- Isotype: IgG1
製品の概要
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製品名
Anti-Cardiac Troponin T antibody [1C11]
Cardiac Troponin T 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [1C11] to Cardiac Troponin T -
由来種
Mouse -
アプリケーション
適用あり: ICC/IF, IHC-P, Sandwich ELISAmore details -
種交差性
交差種: Mouse, Rat, Dog, Human -
免疫原
Recombinant fragment corresponding to Human Cardiac Troponin T aa 150-250.
Database link: P45379 -
ポジティブ･コントロール
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特記事項
This antibody detects Troponin T in human cardiac muscle. No cross-reaction with skeletal troponin T, cTnI and TnC.
This product was changed from ascites to tissue culture supernatant on 17th October 2017 and product received after this date will be from tissue culture supernatant.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
pH: 7.40
Preservative: 0.1% Sodium azide -
Concentration information loading... -
精製度
Protein A purified -
特記事項（精製）
Purified from TCS -
一次抗体 備考
This antibody detects Troponin T in human cardiac muscle. No cross-reaction with skeletal troponin T, cTnI and TnC. -
ポリ/モノ
モノクローナル -
クローン名
1C11 -
ミエローマ
Sp2/0 -
アイソタイプ
IgG1 -
軽鎖の種類
unknown -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab8295の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF | (6) |
Use a concentration of 1 - 5 µg/ml.
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| IHC-P | (2) |
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
| Sandwich ELISA |
Use at an assay dependent concentration.
Can be used as Capture or Detection antibody. |
| 特記事項 |
|---|
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ICC/IF
Use a concentration of 1 - 5 µg/ml. |
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IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
|
Sandwich ELISA
Use at an assay dependent concentration. Can be used as Capture or Detection antibody. |
ターゲット情報
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機能
Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. -
組織特異性
Heart. The fetal heart shows a greater expression in the atrium than in the ventricle, while the adult heart shows a greater expression in the ventricle than in the atrium. Isoform 6 predominates in normal adult heart. Isoforms 1, 7 and 8 are expressed in fetal heart. Isoform 7 is also expressed in failing adult heart. -
関連疾患
Defects in TNNT2 are the cause of cardiomyopathy familial hypertrophic type 2 (CMH2) [MIM:115195]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
Defects in TNNT2 are the cause of cardiomyopathy dilated type 1D (CMD1D) [MIM:601494]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
Defects in TNNT2 are the cause of cardiomyopathy familial restrictive type 3 (RCM3) [MIM:612422]. Restrictive cardiomyopathy is a heart disorder characterized by impaired filling of the ventricles with reduced diastolic volume, in the presence of normal or near normal wall thickness and systolic function. -
配列類似性
Belongs to the troponin T family. - Information by UniProt
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参照データベース
- Entrez Gene: 403532 Dog
- Entrez Gene: 7139 Human
- Entrez Gene: 21956 Mouse
- Entrez Gene: 24837 Rat
- Omim: 191045 Human
- SwissProt: P45379 Human
- SwissProt: P50752 Mouse
- SwissProt: P50753 Rat
see all -
別名
- Cardiac muscle troponin T antibody
- Cardiomyopathy dilated 1D (autosomal dominant) antibody
- Cardiomyopathy hypertrophic 2 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin T antibody [1C11] (ab8295)Ye L et al. Decreased Yes-Associated Protein-1 (YAP1) Expression in Pediatric Hearts with Ventricular Septal Defects. PLoS One 10:e0139712 (2015).
Paraffin-embedded Normal human Heart and iVSD heart tissue (were blocked using 10% FBS for 30 min) stained for Cardiac Troponin T (Red) using ab8295 at 1/200 dilution at room temperature for 2 hours. The slides were then incubated with Fluor® 555-conjugated anti-mouse (Abcam, ab150107; 1:1,000 dilution). The nuclear counterstain was DAPI (Blue).
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![Immunocytochemistry/ Immunofluorescence - Anti-Cardiac Troponin T antibody [1C11] (ab8295)](https://www.abcam.co.jp/ps/products/8/ab8295/Images/ab8295-7-anti-cardiac-troponin-t-antibody-immunocytochemistry-ips-myocytes-human-1c11.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Cardiac Troponin T antibody [1C11] (ab8295)Immunofluorescence staining of Cardiac Troponin T using ab8295 in ioSkeletal Myocytes - Human iPSC-Derived Skeletal Myocytes (ab277612), which were differentiated for 10 days post induction.
The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% PBS-Tween for 5 mins and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab8295 at 5 µg/mL and ab6046, rabbit polyclonal to beta Tubulin, at 1/1000 dilution. Cells were then incubated with ab150117, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150088, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed at 1/1000 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown. Gamma is adjusted to 1.5 in all channels.
The antibody ab8295 gave comparable results using MeOH fixation (100%, 5 min).
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![Sandwich ELISA - Anti-Cardiac Troponin T antibody [1C11] (ab8295)](https://www.abcam.co.jp/ps/products/8/ab8295/Images/ab8295-14115-anti-cardiac-troponin-t-antibody-1c11-sandwich-elisa.jpg)
Sandwich ELISA - Anti-Cardiac Troponin T antibody [1C11] (ab8295)Calibration curves for sandwich cTnT fluoroimmunoassay with different animal TnTs as antigen.(dark blue) canine, (blue/grey) human, (grey) mouse, (black) rat. Monoclonal antibodies: capture, ab8295 [clone 1C11], 1µ -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin T antibody [1C11] (ab8295)](https://www.abcam.co.jp/ps/products/8/ab8295/Images/ab8295-14114-anti-cardiac-troponin-t-antibody-1c11-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin T antibody [1C11] (ab8295)Ab8295 staining human normal heart. Staining is localised to the cytoplasm.
Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS), then incubated with primary antibody for 20 minutes, and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (284)
ab8295 は 284 報の論文で使用されています。
- Harding J et al. Immune-privileged tissues formed from immunologically cloaked mouse embryonic stem cells survive long term in allogeneic hosts. Nat Biomed Eng 8:427-442 (2024). PubMed: 37996616
- Zhang W et al. Cadherin-5 facilitated the differentiation of human induced pluripotent stem cells into sinoatrial node-like pacemaker cells by regulating β-catenin. J Cell Physiol 239:212-226 (2024). PubMed: 38149479
- Swift SK et al. Cardiomyocyte ploidy is dynamic during postnatal development and varies across genetic backgrounds. Development 150:N/A (2023). PubMed: 36912240
- Yu W et al. Sorting nexin 3 exacerbates doxorubicin-induced cardiomyopathy via regulation of TFRC-dependent ferroptosis. Acta Pharm Sin B 13:4875-4892 (2023). PubMed: 38045054
- Cotticelli MG et al. Acute frataxin knockdown in induced pluripotent stem cell-derived cardiomyocytes activates a type I interferon response. Dis Model Mech 16:N/A (2023). PubMed: 36107856