Anti-CARD8 抗体 (ab24186)
Key features and details
- Rabbit polyclonal to CARD8
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-CARD8 antibody
CARD8 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to CARD8 -
由来種
Rabbit -
特異性
ab24186 reacts with CARD8 (CARDINAL/TUCAN) protein. -
アプリケーション
適用あり: ICC/IF, WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to amino acids within the last 50 amino acids of the C terminus of human CARD8, GenBank accession AAG50014
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab24186の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF | (1) |
Use a concentration of 10 µg/ml.
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WB | (1) |
Use a concentration of 2 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 49 kDa).
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特記事項 |
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ICC/IF
Use a concentration of 10 µg/ml. |
WB
Use a concentration of 2 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 49 kDa). |
ターゲット情報
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機能
Inhibits NF-kappa-B activation. May participate in a regulatory mechanism that coordinates cellular responses controlled by NF-kappa-B transcription factor. May be a component of the inflammasome, a protein complex which also includes PYCARD, NALP2 and CASP1 and whose function would be the activation of proinflammatory caspases. -
組織特異性
High expression in lung, ovary, testis and placenta. Lower expression in heart, kidney and liver. Also expressed in spleen, lymph node and bone marrow. -
配列類似性
Contains 1 CARD domain. -
細胞内局在
Cytoplasm. Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 22900 Human
- Omim: 609051 Human
- SwissProt: Q9Y2G2 Human
- Unigene: 446146 Human
- Unigene: 655940 Human
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別名
- Apoptotic protein NDPP 1 antibody
- Apoptotic protein NDPP1 antibody
- CARD 8 antibody
see all
画像
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Lane 1 : Anti-CARD8 antibody (ab24186) at 2 µg/ml (CARD8 expression in K562 cell lysate)
Lane 2 : Anti-CARD8 antibody (ab24186) at 4 µg/ml (CARD8 expression in K562 cell lysate)
All lanes :
Predicted band size: 49 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
A peptide blocking study was also done (data not shown) and the only bands that disappeared were around 45kDa. The other bands are thought to be non-specific, but more testing to confirm or refute this has not been performed. -
ab24186 at 10µg/ml staining CARD8 in K562 cells by ICC/IF
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Immunocytochemistry/ Immunofluorescence - Anti-CARD8 antibody (ab24186)Image courtesy of Dr Mahesh Shivananjappa by Abreview.ab24186 staining CARD8 in human monocytes by Immunocytochemistry/ Immunofluorescence.
Cells were fixed, permeabilized, blocked with 2% BSA for 30 minutes at 25°C and then incubated with ab24186 at a 1/200 dilution for 4 hours at 25°C. The secondary used was an Alexa-Fluor 488 conjugated chicken anti-rabbit IgG (H+L) used at a 1/1000 dilution. -
Immunocytochemistry/ Immunofluorescence - Anti-CARD8 antibody (ab24186)This image is courtesy of an Abreview submitted by Dr Mahesh Shivananjappaab24186 staining CARD8 in Mouse RAW 264.7 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 in 2% BSA and blocked with 2% serum for 1 hour at 22°C. Samples were incubated with primary antibody (1/200 in PBS + 2% BSA) for 18 hours at 4°C. An Alexa Fluor®488-conjugated Chicken anti-rabbit IgG polyclonal (1/750) was used as the secondary antibody.
WGA = Wheat Germ Agglutinin
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (13)
ab24186 は 13 報の論文で使用されています。
- Clark KM et al. Chemical inhibition of DPP9 sensitizes the CARD8 inflammasome in HIV-1-infected cells. Nat Chem Biol 19:431-439 (2023). PubMed: 36357533
- Hsiao JC et al. A ubiquitin-independent proteasome pathway controls activation of the CARD8 inflammasome. J Biol Chem 298:102032 (2022). PubMed: 35580636
- Rao SD et al. M24B aminopeptidase inhibitors selectively activate the CARD8 inflammasome. Nat Chem Biol 18:565-574 (2022). PubMed: 35165443
- Nadkarni R et al. Viral proteases activate the CARD8 inflammasome in the human cardiovascular system. J Exp Med 219:N/A (2022). PubMed: 36129453
- Sharif H et al. Dipeptidyl peptidase 9 sets a threshold for CARD8 inflammasome formation by sequestering its active C-terminal fragment. Immunity 54:1392-1404.e10 (2021). PubMed: 34019797