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Anti-Carboxypeptidase Y 抗体 [10A5B5] (ab113685)

Submit a review Q&A (2)References (7)
Western blot - Anti-Carboxypeptidase Y antibody [10A5B5] (ab113685)

    Key features and details

    • Mouse monoclonal [10A5B5] to Carboxypeptidase Y
    • Suitable for: WB
    • Reacts with: Saccharomyces cerevisiae
    • Isotype: IgG1

    製品の概要

    • 製品名

      Anti-Carboxypeptidase Y antibody [10A5B5]
      Carboxypeptidase Y 一次抗体 製品一覧

    • 製品の詳細

      Mouse monoclonal [10A5B5] to Carboxypeptidase Y

    • 由来種

      Mouse

    • アプリケーション

      適用あり: WBmore details

    • 種交差性

      交差種: Saccharomyces cerevisiae

    • 免疫原

      Full length native protein (purified). This information is considered to be commercially sensitive.

    • ポジティブ･コントロール

      • Yeast membranes

    • 特記事項

      This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

      Product was previously marketed under the MitoSciences sub-brand.

    製品の特性

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab113685の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    WB
    Use a concentration of 0.5 µg/ml. Detects a band of approximately 59 kDa (predicted molecular weight: 59 kDa).
    特記事項
    WB
    Use a concentration of 0.5 µg/ml. Detects a band of approximately 59 kDa (predicted molecular weight: 59 kDa).

    ターゲット情報

    • 機能

      Involved in degradation of small peptides. Digests preferentially peptides containing an aliphatic or hydrophobic residue in P1' position, as well as methionine, leucine or phenylalanine in P1 position of ester substrate.

    • 配列類似性

      Belongs to the peptidase S10 family.

    • 翻訳後修飾

      Enters the endoplasmic reticulum as an inactive zymogen and is modified by four N-linked core oligosaccharides, giving rise to a precursor known as P1 (67 kDa). As P1 transits through the Golgi, extension of its core oligosaccharides leads to the Golgi-modified P2 precursor (69 kDa). P2 is sorted away from secretory proteins at or beyond a late Golgi compartment and is subsequently delivered to the vacuole via a prevacuolar endosome-like compartment. Upon arrival in the vacuole, the N-terminal prosegment of P2 is cleaved to yield the enzymatically active mature vacuolar form of CPY (61 kDa).

    • 細胞内局在

      Vacuole. Lysosome-like vacuoles.
    • Information by UniProt

    • 参照データベース

      • 別名

        • carboxypeptidase C PRC1 antibody
        • Carboxypeptidase Y antibody
        • Carboxypeptidase YSCY antibody
        • CBPY_YEAST antibody
        • CPY antibody
        • LBC1 antibody
        • PRC1 antibody
        • Vacuolar carboxypeptidase Y antibody
        see all

      画像

      • Western blot - Anti-Carboxypeptidase Y antibody [10A5B5] (ab113685)
        Western blot - Anti-Carboxypeptidase Y antibody [10A5B5] (ab113685)
        All lanes : Anti-Carboxypeptidase Y antibody [10A5B5] (ab113685)

        Lane 1 : Yeast whole cell lysate
        Lane 2 : Yeast membrane preparation

        Lysates/proteins at 15 µg per lane.

        Developed using the ECL technique.

        Performed under reducing conditions.

        Predicted band size: 59 kDa

      参考文献 (7)

      ab113685 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab113685 は 7 報の論文で使用されています。

      • Sun D  et al. The cryo-EM structure of the SNX-BAR Mvp1 tetramer. Nat Commun 11:1506 (2020). PubMed: 32198400
      • Yanguas F  et al. Ent3 and GGA adaptors facilitate diverse anterograde and retrograde trafficking events to and from the prevacuolar endosome. Sci Rep 9:10747 (2019). PubMed: 31341193
      • Aviram N  et al. The SND proteins constitute an alternative targeting route to the endoplasmic reticulum. Nature 540:134-138 (2016). PubMed: 27905431
      • Ast T  et al. A cytosolic degradation pathway, prERAD, monitors pre-inserted secretory pathway proteins. J Cell Sci 127:3017-23 (2014). WB ; Saccharomyces cerevisiae . PubMed: 24849653
      • Manandhar SP  et al. Saccharomyces cerevisiae Env7 is a novel serine/threonine kinase 16-related protein kinase and negatively regulates organelle fusion at the lysosomal vacuole. Mol Cell Biol 33:526-42 (2013). PubMed: 23166297
      • Chen J  et al. Accumulation of an antidepressant in vesiculogenic membranes of yeast cells triggers autophagy. PLoS One 7:e34024 (2012). PubMed: 22529904
      • Li L  et al. A Role for Iron-Sulfur Clusters in the Regulation of Transcription Factor Yap5-dependent High Iron Transcriptional Responses in Yeast. J Biol Chem 287:35709-21 (2012). WB ; Saccharomyces cerevisiae . PubMed: 22915593

      レビューと Q&A

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      1-2 of 2 Abreviews or Q&A

      Question

      W303 background yeast strains: wild type, prc1∆ (neg control), Prc1-13xMYC (pos control) and a sec65-1 mutant.  From overnight cultures, strains were grown @30degC from OD = 0.3 to 0.6, then shifted to 38degC for 45'.  6OD pellets were collected and 0.5OD were run on SDS-PAGE (4-20% gradient).  Abcam34636 was diluted 1:5000 in 5% milk/PBS with 0.01% tween and incubated overnight 4degC.  An HRP-conj. goat anti-rabbit from SCBT was used at 1:10000 for 1 hr.  Let me know if you have any further questions.  

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      Answer

      Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number xxxxx. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.

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      Question

      We used the anti-CPY antibody for WB in S. cerevisiae, which cross-reacted with all of our sample lanes (including our negative control, prc1∆, which is genetically lacking the target antigen). I can send you images of the data if you would like. For now, we are purchasing the anti-CPY [10A5B5] monoclonal antibody (ab113685). Hopefully this works better. If you could recover the cost difference between the two items, that would be great. Please let me know if you need any info (i.e. PO #) from either transaction.

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      Answer

      Thank you for your reply.

      I would be happy to create a credit note/refund for your purchase of ab34636. In order to do this could you provide the PO used for that transaction? It is also very helpful for our internal testing if you could provide the details of your western blot protocol? Images are very helpful as well and I thank you for your offer of them.

      I hope this information is helpful to you and look forward to your reply. Please do not hesitate to contact us if you need any more advice or information.

      Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

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