Anti-Carboxypeptidase B/CPB 抗体 (ab153716)
Key features and details
- Rabbit polyclonal to Carboxypeptidase B/CPB
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-Carboxypeptidase B/CPB antibody
Carboxypeptidase B/CPB 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Carboxypeptidase B/CPB -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Mouse, Rat, Human
交差が予測される動物種: Dog, Pig
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免疫原
Recombinant fragment corresponding to Human Carboxypeptidase B/CPB aa 144-417.
Database link: P15086 -
ポジティブ･コントロール
- WB: A549, HeLa, AsPC-1, HepG2, transfected 293T and HCT116 whole cell lysates, mouse and rat tissue lysate; IHC-P: Human Ca922 xenograft, mouse and rat pancreas tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab153716の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 47 kDa.
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| IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 特記事項 |
|---|
|
WB
1/500 - 1/3000. Predicted molecular weight: 47 kDa. |
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IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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組織特異性
Pancreas. -
配列類似性
Belongs to the peptidase M14 family. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 403512 Dog
- Entrez Gene: 1360 Human
- Entrez Gene: 76703 Mouse
- Entrez Gene: 397341 Pig
- Entrez Gene: 24271 Rat
- Omim: 114852 Human
- SwissProt: P55261 Dog
- SwissProt: P15086 Human
see all -
別名
- Carboxypeptidase B antibody
- Carboxypeptidase B1 (tissue) antibody
- Carboxypeptidase B1 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carboxypeptidase B/CPB antibody (ab153716)
Immunohistochemical analysis of paraffin-embedded rat pancreas tissue, labeling Carboxypeptidase B/CPB with ab153716 at 1/1000 dilution. Antigen Retrieval performed using citrate buffer, pH 6.0, 15 min
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Western blot - Anti-Carboxypeptidase B/CPB antibody (ab153716)All lanes : Anti-Carboxypeptidase B/CPB antibody (ab153716) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : AsPC-1 cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 47 kDa -
Western blot - Anti-Carboxypeptidase B/CPB antibody (ab153716)All lanes : Anti-Carboxypeptidase B/CPB antibody (ab153716) at 1/5000 dilution
Lane 1 : Non-transfected 293T cell lysate
Lane 2 : Carboxypeptidase B/CPB transfected 293T cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 47 kDa -
Western blot - Anti-Carboxypeptidase B/CPB antibody (ab153716)Anti-Carboxypeptidase B/CPB antibody (ab153716) at 1/50000 dilution + Mouse pancreas tissue lysate at 50 µg
Secondary
HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 47 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carboxypeptidase B/CPB antibody (ab153716)Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue, labeling Carboxypeptidase B/CPB with ab153716 at 1/1000 dilution. Antigen Retrieval performed using citrate buffer, pH 6.0, 15 min
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Western blot - Anti-Carboxypeptidase B/CPB antibody (ab153716)Anti-Carboxypeptidase B/CPB antibody (ab153716) at 1/50000 dilution + Rat pancreas tissue lysate at 50 µg
Secondary
HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 47 kDa -
Western blot - Anti-Carboxypeptidase B/CPB antibody (ab153716)Anti-Carboxypeptidase B/CPB antibody (ab153716) at 1/1000 dilution + HepG2 cell lysate at 30 µg
Predicted band size: 47 kDa
10% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carboxypeptidase B/CPB antibody (ab153716)Immunohistochemical analysis of paraffin-embedded Human Ca922 xenograft tissue, labeling Carboxypeptidase B/CPB with ab153716 at 1/250 dilution.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab153716 は論文での使用が確認できていません。