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リコンビナントRabMAb

Anti-Carbonic Anhydrase 9/CA9 抗体 [SP106] (ab105226)

Submit a review Q&A (2)
Flow Cytometry - Anti-Carbonic Anhydrase 9/CA9 antibody [SP106] (ab105226)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 9/CA9 antibody [SP106] (ab105226)
  • Anti-Carbonic Anhydrase 9/CA9 antibody [SP106] (ab105226)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP106] to Carbonic Anhydrase 9/CA9
  • Suitable for: IHC-P, Flow Cyt
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alkaline Phosphatase Carrier Free

製品の概要

  • 製品名

    Anti-Carbonic Anhydrase 9/CA9 antibody [SP106]
    Carbonic Anhydrase 9/CA9 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [SP106] to Carbonic Anhydrase 9/CA9

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: IHC-P, Flow Cytmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Synthetic peptide within Human Carbonic Anhydrase 9/CA9 aa 400 to the C-terminus (internal sequence). The exact sequence is proprietary.
    Database link: Q16790

  • ポジティブ･コントロール

    • Human stomach tissue; Ramos cell lysate

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab105226の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P
1/100. Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
Flow Cyt
Use at an assay dependent concentration.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
特記事項
IHC-P
1/100. Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
Flow Cyt
Use at an assay dependent concentration.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能

    Reversible hydration of carbon dioxide. Participates in pH regulation. May be involved in the control of cell proliferation and transformation. Appears to be a novel specific biomarker for a cervical neoplasia.

  • 組織特異性

    Expressed primarily in carcinoma cells lines. Expression is restricted to very few normal tissues and the most abundant expression is found in the epithelial cells of gastric mucosa.

  • 配列類似性

    Belongs to the alpha-carbonic anhydrase family.
    Contains 1 alpha-carbonic anhydrase domain.

  • 翻訳後修飾

    Asn-346 bears high-mannose type glycan structures.

  • 細胞内局在

    Nucleus. Nucleus, nucleolus. Cell membrane. Cell projection, microvillus membrane. Found on the surface microvilli and in the nucleus, particularly in nucleolus.
  • Information by UniProt

  • 参照データベース

    • 別名

      • CA-IX antibody
      • CA9 antibody
      • CAH9_HUMAN antibody
      • CAIX antibody
      • Carbonate dehydratase IX antibody
      • Carbonic anhydrase 9 antibody
      • Carbonic anhydrase IX antibody
      • Carbonic dehydratase antibody
      • G250 antibody
      • Membrane antigen MN antibody
      • MN antibody
      • P54/58N antibody
      • pMW1 antibody
      • RCC associated protein G250 antibody
      • RCC-associated antigen G250 antibody
      • Renal cell carcinoma-associated antigen G250 antibody
      see all

    画像

    • Flow Cytometry - Anti-Carbonic Anhydrase 9/CA9 antibody [SP106] (ab105226)
      Flow Cytometry - Anti-Carbonic Anhydrase 9/CA9 antibody [SP106] (ab105226)

      Flow cytometric analysis of rabbit anti-Carbonic Anhydrase 9/CA9 (SP106) antibody ab105226 (1/100) in HT29 cells (green) compared to negative control of rabbit IgG (blue).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 9/CA9 antibody [SP106] (ab105226)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 9/CA9 antibody [SP106] (ab105226)

      ab105226, at 1/100 dilution, staining Carbonic Anhydrase 9/CA9 in formalin-fixed, paraffin-embedded Human stomach tissue by Immunohistochemistry.

    • Anti-Carbonic Anhydrase 9/CA9 antibody [SP106] (ab105226)
      Anti-Carbonic Anhydrase 9/CA9 antibody [SP106] (ab105226)

    参考文献 (0)

    ab105226 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab105226 は論文での使用が確認できていません。

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-2 of 2 Abreviews or Q&A

    Question

    I was wondering if you offer a sample size for your carbonic anhydrase IX monoclonal antibody? I was hoping to test out the antibody prior to purchasing it.

    Read More
    Answer

    Thank you for contacting us. Because we carry over 70,000 products, it isn't feasible for us to keep small sample sizes of our products.

    We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase.

    If the product is to be used in an untested species or application, you may be eligible for our testing discount program if the antibody has not yet been purchased. Please contact our Scientific Support team by replying to this email prior to purchase for more information.

    Otherwise, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.

    To find out more about our Abreview system, please see the following link:

    https://www.abcam.com/abreviews

    I hope this information is helpful. Please do not hesitate to contact us again with any other questions.

    Read More

    Question

    Bonjour Mme ******* de ******* (Maroc) nous a livré deux anticorps que nous avons demandés pour un projet : HIF 1 alpha et CAIX; la boite ne contient que les anticorps concentrés sans diluent et sans anticorps secondaires.Pour le diluent nous pouvons éventuellement utiliser le TBS comme c'est conseillé sur votre site mais pour l'anticorps secondaire qu'elle serait la solution?

    Read More
    Answer

    Merci de nous avoir contactés. Les anticorps primaires Abcam sont livrés sans diluent ni anticorps secondaire. J'ai retrouvé la commande passée par ****. Cette commande concerne en effet 2 anticorps primaires, l'anti-HIF1 alpha antibody [ESEE122]référence ab8366 et l'anti-Carbonic Anhydrase IX antibody [SP106]référence ab105226. Le lien vers la liste des anticorps secondaires compatibles avec ab8366 (Mouse IgG1)est : https://www.abcam.com/index.html?pageconfig=searchresults&search=IgG1&pt=7&sk=targsp&sv=2&sn=Mouse&l=0&fViewMore=1 Le lien vers la liste des anticorps secondaires compatibles avec ab105226 (Rabbit IgG) est : https://www.abcam.com/index.html?pageconfig=searchresults&search=IgG&pt=7&sk=targsp&sv=2&sn=Rabbit&l=0&fViewMore=1 Vous pouvez affiner votre recherche en utilisant les filtres à gauche de la liste (application, conjugué, etc). La solution de dilution dépendra de l'application testée. J'espère que ces informations vous seront utiles. N'hésitez pas à nous contacter de nouveau si vous avez d'autres questions. Bonne et heureuse année 2012 à vouségalement.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com