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リコンビナントRabMAb

Anti-Carbonic Anhydrase 9/CA9 抗体 [EPR4151(2)] - BSA and Azide free (ab180539)

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Western blot - Anti-Carbonic Anhydrase 9/CA9 antibody [EPR4151(2)] - BSA and Azide free (ab180539)
  • Western blot - Anti-Carbonic Anhydrase 9/CA9 antibody [EPR4151(2)] - BSA and Azide free (ab180539)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 9/CA9 antibody [EPR4151(2)] - BSA and Azide free (ab180539)
  • Anti-Carbonic Anhydrase 9/CA9 antibody [EPR4151(2)] - BSA and Azide free (ab180539)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4151(2)] to Carbonic Anhydrase 9/CA9 - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 568 Unconjugated

製品の概要

  • 製品名

    Anti-Carbonic Anhydrase 9/CA9 antibody [EPR4151(2)] - BSA and Azide free
    Carbonic Anhydrase 9/CA9 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR4151(2)] to Carbonic Anhydrase 9/CA9 - BSA and Azide free

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: IHC-P, WBmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Synthetic peptide within Human Carbonic Anhydrase 9/CA9 aa 200-300 (extracellular). The exact sequence is proprietary.
    Database link: Q16790

  • ポジティブ･コントロール

    • IHC: Human stomach tissue; WB: HT-29 cells and human stomach lysate.

  • 特記事項

    ab180539 is the carrier-free version of ab108351.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab180539の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.
WB
Use at an assay dependent concentration. Predicted molecular weight: 49 kDa.
特記事項
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.
WB
Use at an assay dependent concentration. Predicted molecular weight: 49 kDa.

ターゲット情報

  • 機能

    Reversible hydration of carbon dioxide. Participates in pH regulation. May be involved in the control of cell proliferation and transformation. Appears to be a novel specific biomarker for a cervical neoplasia.

  • 組織特異性

    Expressed primarily in carcinoma cells lines. Expression is restricted to very few normal tissues and the most abundant expression is found in the epithelial cells of gastric mucosa.

  • 配列類似性

    Belongs to the alpha-carbonic anhydrase family.
    Contains 1 alpha-carbonic anhydrase domain.

  • 翻訳後修飾

    Asn-346 bears high-mannose type glycan structures.

  • 細胞内局在

    Nucleus. Nucleus, nucleolus. Cell membrane. Cell projection, microvillus membrane. Found on the surface microvilli and in the nucleus, particularly in nucleolus.
  • Information by UniProt

  • 参照データベース

    • 別名

      • CA-IX antibody
      • CA9 antibody
      • CAH9_HUMAN antibody
      • CAIX antibody
      • Carbonate dehydratase IX antibody
      • Carbonic anhydrase 9 antibody
      • Carbonic anhydrase IX antibody
      • Carbonic dehydratase antibody
      • G250 antibody
      • Membrane antigen MN antibody
      • MN antibody
      • P54/58N antibody
      • pMW1 antibody
      • RCC associated protein G250 antibody
      • RCC-associated antigen G250 antibody
      • Renal cell carcinoma-associated antigen G250 antibody
      see all

    画像

    • Western blot - Anti-Carbonic Anhydrase 9/CA9 antibody [EPR4151(2)] - BSA and Azide free (ab180539)
      Western blot - Anti-Carbonic Anhydrase 9/CA9 antibody [EPR4151(2)] - BSA and Azide free (ab180539)
      Anti-Carbonic Anhydrase 9/CA9 antibody [EPR4151(2)] (ab108351) at 1/10000 dilution (Purified) + HT-29 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 49 kDa
      Observed band size: 54, 58 kDa why is the actual band size different from the predicted?



      This data was developed using ab108351, the same antibody clone in a different buffer formulation.

      The molecular weight observed is consistent with what has been described in the literature (PMID: 12966427, 30734594 and 15951534).

      Blocking Buffer and concentration: 5% NFDM/TBST

    • Western blot - Anti-Carbonic Anhydrase 9/CA9 antibody [EPR4151(2)] - BSA and Azide free (ab180539)
      Western blot - Anti-Carbonic Anhydrase 9/CA9 antibody [EPR4151(2)] - BSA and Azide free (ab180539)
      Anti-Carbonic Anhydrase 9/CA9 antibody [EPR4151(2)] (ab108351) at 1/10000 dilution (Purified) + Human stomach lysate at 20 µg

      Secondary
      Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

      Predicted band size: 49 kDa
      Observed band size: 54, 58 kDa why is the actual band size different from the predicted?



      This data was developed using ab108351, the same antibody clone in a different buffer formulation.

      The molecular weight observed is consistent with what has been described in the literature (PMID: 12966427, 30734594 and 15951534).

      Blocking Buffer and concentration: 5% NFDM/TBST

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 9/CA9 antibody [EPR4151(2)] - BSA and Azide free (ab180539)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 9/CA9 antibody [EPR4151(2)] - BSA and Azide free (ab180539)

      This data was developed using ab180539, the same antibody clone in a different buffer formulation.
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach tissue sections labeling Carbonic Anhydrase 9/CA9 with Purified ab180539 at 1:150 dilution (0.89 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    • Anti-Carbonic Anhydrase 9/CA9 antibody [EPR4151(2)] - BSA and Azide free (ab180539)
      Anti-Carbonic Anhydrase 9/CA9 antibody [EPR4151(2)] - BSA and Azide free (ab180539)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (1)

    ab180539 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab180539 は 1 報の論文で使用されています。

    • Askoxylakis V  et al. Binding of the Phage Display Derived Peptide CaIX-P1 on Human Colorectal Carcinoma Cells Correlates with the Expression of Carbonic Anhydrase IX. Int J Mol Sci 13:13030-48 (2012). WB ; Human . PubMed: 23202936

    レビューと Q&A

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    レビューを送る 質問を送る

    Immunohistochemistry (Frozen sections) abreview for Anti-Carbonic Anhydrase 9/CA9 antibody [EPR4151(2)] - BSA and Azide free

     Good
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (Glioblastoma multiforme)
    Permeabilization
    Yes - 0.25% TritonX-100
    Specification
    Glioblastoma multiforme
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
    Fixative
    Acetone + PFA
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Oct 24 2022

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