Anti-Carbonic Anhydrase 9/CA9 抗体 (ab184006)
Key features and details
- Rabbit polyclonal to Carbonic Anhydrase 9/CA9
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
-
製品名
Anti-Carbonic Anhydrase 9/CA9 antibody
Carbonic Anhydrase 9/CA9 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Carbonic Anhydrase 9/CA9 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WB, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment within Human Carbonic Anhydrase 9/CA9 aa 1-168. The exact sequence is proprietary.
Database link: Q16790 -
ポジティブ･コントロール
- HepG2 cell lysate, treated with 200µM CoCl2 for 24hr; Human cervical carcinoma tissue; CoCl2- treated A431 cells.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab184006の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
1/100 - 1/1000.
|
|
| WB | (1) |
1/500 - 1/3000. Predicted molecular weight: 50 kDa.
|
| ICC/IF |
1/100 - 1/1000.
|
| 特記事項 |
|---|
|
IHC-P
1/100 - 1/1000. |
|
WB
1/500 - 1/3000. Predicted molecular weight: 50 kDa. |
|
ICC/IF
1/100 - 1/1000. |
ターゲット情報
-
機能
Reversible hydration of carbon dioxide. Participates in pH regulation. May be involved in the control of cell proliferation and transformation. Appears to be a novel specific biomarker for a cervical neoplasia. -
組織特異性
Expressed primarily in carcinoma cells lines. Expression is restricted to very few normal tissues and the most abundant expression is found in the epithelial cells of gastric mucosa. -
配列類似性
Belongs to the alpha-carbonic anhydrase family.
Contains 1 alpha-carbonic anhydrase domain. -
翻訳後修飾
Asn-346 bears high-mannose type glycan structures. -
細胞内局在
Nucleus. Nucleus, nucleolus. Cell membrane. Cell projection, microvillus membrane. Found on the surface microvilli and in the nucleus, particularly in nucleolus. - Information by UniProt
-
参照データベース
- Entrez Gene: 768 Human
- Omim: 603179 Human
- SwissProt: Q16790 Human
- Unigene: 63287 Human
-
別名
- CA-IX antibody
- CA9 antibody
- CAH9_HUMAN antibody
see all
画像
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 9/CA9 antibody (ab184006)
Immunohistochemical analysis of paraffin-embedded Human cervical carcinoma tissue labeling Carbonic Anhydrase 9/CA9 with ab184006 at 1/500 dilution.
-
Western blot - Anti-Carbonic Anhydrase 9/CA9 antibody (ab184006)All lanes : Anti-Carbonic Anhydrase 9/CA9 antibody (ab184006) at 1/1000 dilution
Lane 1 : HepG2 whole cell lysate, untreated
Lane 2 : HepG2 whole cell lysate, treated with 200 µM CoCl2 for 24 hr
Lysates/proteins at 30 µg per lane.
Predicted band size: 50 kDa10% SDS PAGE
-
Immunocytochemistry/ Immunofluorescence - Anti-Carbonic Anhydrase 9/CA9 antibody (ab184006)Immunofluorescent analysis of A431 cells labeling Carbonic Anhydrase 9/CA9 with ab184006 at 1/500 dilution. A431 cells
were fixed in ice-cold MeOH for 5 min. Cells were treated with 200 μM CoCl2 (right) or untreated (left)
Green: Carbonic Anhydrase 9/CA9 protein showing membrane staining.
Blue: Hoechst 33342.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (15)
ab184006 は 15 報の論文で使用されています。
- Liu K et al. Novel miRNA-based drug CD5-2 reduces liver tumor growth in diethylnitrosamine-treated mice by normalizing tumor vasculature and altering immune infiltrate. Front Immunol 14:1245708 (2023). PubMed: 37795103
- Yadav P et al. Hypoxia-induced loss of SRSF2-dependent DNA methylation promotes CTCF-mediated alternative splicing of VEGFA in breast cancer. iScience 26:106804 (2023). PubMed: 37235058
- Bess SN et al. Macrophage-targeted anti-CCL2 immunotherapy enhances tumor sensitivity to 5-fluorouracil in a Balb/c-CT26 murine colon carcinoma model measured using diffuse reflectance spectroscopy. BMC Immunol 23:20 (2022). PubMed: 35461243
- Qorri B et al. Repositioning of Old Drugs for Novel Cancer Therapies: Continuous Therapeutic Perfusion of Aspirin and Oseltamivir Phosphate with Gemcitabine Treatment Disables Tumor Progression, Chemoresistance, and Metastases. Cancers (Basel) 14:N/A (2022). PubMed: 35892853
- Suzuki H et al. Efficacy and tolerability of Sorafenib plus metronomic chemotherapy S-1 for advanced hepatocellular carcinoma in preclinical and clinical assessments. Transl Oncol 14:101201 (2021). PubMed: 34388691