Anti-Carbonic Anhydrase 3/CA3 抗体 (ab175309)
Key features and details
- Rabbit polyclonal to Carbonic Anhydrase 3/CA3
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-Carbonic Anhydrase 3/CA3 antibody
Carbonic Anhydrase 3/CA3 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Carbonic Anhydrase 3/CA3 -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, IHC-P, WBmore details -
種交差性
交差種: Rat, Human
交差が予測される動物種: Horse, Pig
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免疫原
Recombinant full length protein corresponding to Human Carbonic Anhydrase 3/CA3 aa 1-260.
Sequence:MAKEWGYASHNGPDHWHELFPNAKGENQSPVELHTKDIRHDPSLQPWSVS YDGGSAKTILNNGKTCRVVFDDTYDRSMLRGGPLPGPYRLRQFHLHWGSS DDHGSEHTVDGVKYAAELHLVHWNPKYNTFKEALKQRDGIAVIGIFLKIG HENGEFQIFLDALDKIKTKGKEAPFTKFDPSCLFPACRDYWTYQGSFTTP PCEECIVWLLLKEPMTVSSDQMAKLRSLLSSAENEPPVPLVSNWRPPQPI NNRVVRASFK
Database link: P07451 -
ポジティブ･コントロール
- Recombinant Human Carbonic Anhydrase 3/CA3 protein (BSA and azide free) (ab173078) can be used as a positive control in WB. HeLa and 293 cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 49% PBS -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab175309の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
Use at an assay dependent concentration.
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| IHC-P |
1/50 - 1/200.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
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| WB |
1/500 - 1/2000. Predicted molecular weight: 30 kDa.
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| 特記事項 |
|---|
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ICC/IF
Use at an assay dependent concentration. |
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IHC-P
1/50 - 1/200. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB
1/500 - 1/2000. Predicted molecular weight: 30 kDa. |
ターゲット情報
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機能
Reversible hydration of carbon dioxide. -
組織特異性
Muscle specific. -
配列類似性
Belongs to the alpha-carbonic anhydrase family. -
発生段階
At 6 weeks gestation, transcripts accumulate at low levels in the somites and at high levels throughout the notochord. As gestation continues, CA3 becomes abundant in all developing muscle masses and continues at high to moderate levels in the notochord. -
翻訳後修飾
S-glutathionylated in hepatocytes under oxidative stress. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 100050125 Horse
- Entrez Gene: 761 Human
- Entrez Gene: 494016 Pig
- Entrez Gene: 54232 Rat
- Omim: 114750 Human
- SwissProt: P07450 Horse
- SwissProt: P07451 Human
- SwissProt: Q5S1S4 Pig
see all -
別名
- CA III antibody
- CA-III antibody
- Ca3 antibody
see all
画像
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Western blot - Anti-Carbonic Anhydrase 3/CA3 antibody (ab175309)All lanes : Anti-Carbonic Anhydrase 3/CA3 antibody (ab175309) at 1/500 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293 cell lysate
Predicted band size: 30 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 3/CA3 antibody (ab175309)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat testis tissue labelling Carbonic Anhydrase 3/CA3 with ab175309 at 1/200. Magnification: 200x.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 3/CA3 antibody (ab175309)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat testis tissue labelling Carbonic Anhydrase 3/CA3 with ab175309 at 1/200. Magnification: 400x.
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Immunocytochemistry/ Immunofluorescence - Anti-Carbonic Anhydrase 3/CA3 antibody (ab175309)Immunocytochemistry/Immunofluorescence analysis of HeLa cells using ab175309. Blue DAPI for nuclear staining.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab175309 は論文での使用が確認できていません。