AB108367

Anti-Carbonic Anhydrase 1/CA1 抗体 [EPR5193]

Name: Anti-Carbonic Anhydrase 1/CA1 抗体 [EPR5193] (ab108367) | Abcam
Brand: Abcam Limited
SKU: AB108367
Price: 30000 JPY
Availability: InStock
Rating: 5 (2 reviews)

Anti-Carbonic Anhydrase 1/CA1 antibody [EPR5193]

20ul selling size
RabMAb
Recombinant
詳細を見る

(2 Reviews)

(10 Publications)

Rabbit Recombinant Monoclonal CAH1 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Mouse, Rat, Zebrafish, Human samples. Cited in 10 publications.

別名を表示する

Carbonic anhydrase 1, Carbonate dehydratase I, Carbonic anhydrase B, Carbonic anhydrase I, Cyanamide hydratase CA1, CAB, CA-I, CA1

3 Images

Flow Cytometry (Intracellular) - Anti-Carbonic Anhydrase 1/CA1 antibody [EPR5193] (AB108367)

Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-Carbonic Anhydrase 1/CA1 antibody [EPR5193] (AB108367)

Intracellular Flow Cytometry analysis of K-562 (Human chronic myelogenous leukemia lymphoblast) cells labeling Carbonic Anhydrase I/CA1 with Purified ab108367 at 1/60 dilution (10μg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluorr^® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

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Western blot - Anti-Carbonic Anhydrase 1/CA1 antibody [EPR5193] (AB108367)

All lanes:

Western blot - Anti-Carbonic Anhydrase 1/CA1 antibody [EPR5193] (ab108367) at 1/10000 dilution

Lane 1:

K562 cell lysates at 10 µg

Lane 2:

TF1 cell lysates at 10 µg

Predicted band size: 28 kDa

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Lab

Western blot - Anti-Carbonic Anhydrase 1/CA1 antibody [EPR5193] (AB108367)

All lanes:

Western blot - Anti-Carbonic Anhydrase 1/CA1 antibody [EPR5193] (ab108367) at 1/10000 dilution

Lane 1:

TF-1 (Human Erythroleukemia erythroblast) whole cell lysates at 20 µg

Lane 2:

Mouse spleen lysates at 20 µg

Lane 3:

Rat brain lysates at 20 µg

Lane 4:

Rat spleen lysates at 20 µg

Lane 5:

Zebrafish eye lysates at 20 µg

Lane 6:

Mouse kidney lysates at 20 µg

Lane 7:

HEL (Human Erythroleukemia erythroblast) whole cell lysates at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 28 kDa

Observed band size: 29 kDa

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Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR5193

アイソタイプ

IgG

キャリアフリー

交差種

Mouse, Rat, Human, Zebrafish

アプリケーション

WB, Flow Cyt (Intra)

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/60", "FlowCytIntra-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Zebrafish": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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製品の詳細

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Conditional Ambient

短期保存温度

+4°C

長期保存温度

-20°C

保管に関する情報

Stable for 12 months at -20°C

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Catalyzes the reversible hydration of carbon dioxide (PubMed : 10550681, PubMed : 16506782, PubMed : 16686544, PubMed : 16807956, PubMed : 17127057, PubMed : 17314045, PubMed : 17407288, PubMed : 18618712, PubMed : 19186056, PubMed : 19206230). Can hydrate cyanamide to urea (PubMed : 10550681).

See full target information Carbonic anhydrase 1

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文献 (10)

Recent publications for all applications. Explore the full list and refine your search

Acta neuropathologica communications 11:59 PubMed37013604

2023

Upregulation of carbonic anhydrase 1 beneficial for depressive disorder.

Applications

Unspecified application

Species

Unspecified reactive species

Ke Cheng,Yue Wang,Yong He,Yu Tian,Junjie Li,Chong Chen,Xingzhe Xu,Zhonghao Wu,Heming Yu,Xiangyu Chen,Yili Wu,Weihong Song,Zhifang Dong,Huatai Xu,Peng Xie

Microorganisms 10: PubMed35456874

2022

Red Blood Cell BCL-x Is Required for Survival: Insights into Host-Directed Malaria Therapies.

Applications

Unspecified application

Species

Unspecified reactive species

Coralie Boulet,Ghizal Siddiqui,Taylah L Gaynor,Christian Doerig,Darren J Creek,Teresa G Carvalho

Biomolecules 12: PubMed35204826

2022

A Potential Citrate Shunt in Erythrocytes of PKAN Patients Caused by Mutations in Pantothenate Kinase 2.

Applications

Unspecified application

Species

Unspecified reactive species

Maike Werning,Verena Dobretzberger,Martin Brenner,Ernst W Müllner,Georg Mlynek,Kristina Djinovic-Carugo,David M Baron,Lena Fragner,Almut T Bischoff,Boriana Büchner,Thomas Klopstock,Wolfram Weckwerth,Ulrich Salzer

Diabetes 71:795-811 PubMed35043173

2022

Methazolamide Attenuates the Development of Diabetic Cardiomyopathy by Promoting β-Catenin Degradation in Type 1 Diabetic Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoqing Chen,Yilang Li,Xun Yuan,Wenchang Yuan,Conglin Li,Yue Zeng,Yuling Lian,Xiaoxia Qiu,Yuan Qin,Guiping Zhang,Xiawen Liu,Chengfeng Luo,Jian-Dong Luo,Ning Hou

Cell stem cell 29:101-115.e10 PubMed34582804

2021

SATB2 preserves colon stem cell identity and mediates ileum-colon conversion via enhancer remodeling.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Gu,Hua Wang,Xiaofeng Huang,Judith Kraiczy,Pratik N P Singh,Charles Ng,Sezin Dagdeviren,Sean Houghton,Oscar Pellon-Cardenas,Ying Lan,Yaohui Nie,Jiaoyue Zhang,Kushal K Banerjee,Emily J Onufer,Brad W Warner,Jason Spence,Ellen Scherl,Shahin Rafii,Richard T Lee,Michael P Verzi,David Redmond,Randy Longman,Kristian Helin,Ramesh A Shivdasani,Qiao Zhou

Annals of clinical and translational neurology 7:1340-1351 PubMed32705819

2020

PKAN neurodegeneration and residual PANK2 activities in patient erythrocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Maike Werning,Ernst W Müllner,Georg Mlynek,Verena Dobretzberger,Kristina Djinovic-Carugo,David M Baron,Holger Prokisch,Boriana Büchner,Thomas Klopstock,Ulrich Salzer

Frontiers in pharmacology 10:766 PubMed31354482

2019

Carbonic Anhydrase 1-Mediated Calcification Is Associated With Atherosclerosis, and Methazolamide Alleviates Its Pathogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Lin Yuan,Minghua Wang,Tianqi Liu,Yinsheng Lei,Qiang Miao,Quan Li,Hongxing Wang,Guoqing Zhang,Yinglong Hou,Xiaotian Chang

Molecular omics 14:181-196 PubMed29770421

2018

Quantitative proteomics and systems analysis of cultured H9C2 cardiomyoblasts during differentiation over time supports a 'function follows form' model of differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Cynthia Kankeu,Kylie Clarke,Delphi Van Haver,Kris Gevaert,Francis Impens,Anna Dittrich,H Llewelyn Roderick,Egle Passante,Heinrich J Huber

International journal of molecular sciences 17: PubMed27809276

2016

Expression of Carbonic Anhydrase I in Motor Neurons and Alterations in ALS.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaochen Liu,Deyi Lu,Robert Bowser,Jian Liu

The Journal of cell biology 210:1075-83 PubMed26416961

2015

Müller glia provide essential tensile strength to the developing retina.

Applications

Unspecified application

Species

Unspecified reactive species

Ryan B MacDonald,Owen Randlett,Julia Oswald,Takeshi Yoshimatsu,Kristian Franze,William A Harris

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Carbonic Anhydrase 1/CA1 抗体 [EPR5193]

Anti-Carbonic Anhydrase 1/CA1 antibody [EPR5193]

Flow Cytometry (Intracellular) - Anti-Carbonic Anhydrase 1/CA1 antibody [EPR5193] (AB108367)

Western blot - Anti-Carbonic Anhydrase 1/CA1 antibody [EPR5193] (AB108367)

Western blot - Anti-Carbonic Anhydrase 1/CA1 antibody [EPR5193] (AB108367)

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Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

代替製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

組合せ製品

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存温度

長期保存温度

保管に関する情報

製品プロトコール

ターゲットの情報

文献 (10)

Abcam product promise