Anti-carabin 抗体 (ab77625)
Key features and details
- Goat polyclonal to carabin
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-carabin antibody
carabin 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to carabin -
由来種
Goat -
アプリケーション
適用あり: Flow Cyt (Intra), WBmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Horse, Dog, Rhesus monkey
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免疫原
Synthetic peptide:
C-PRRKPQTRGKTFH
, corresponding to internal sequence amino acids 405-417 of Human carabin (NP_940919.1). -
ポジティブ･コントロール
- Human spleen lysates Flow Cyt (Intra): Jurkat cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 0.5% Tris buffered saline -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab77625 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab77625の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt (Intra) |
Use a concentration of 10 µg/ml.
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| WB |
Use a concentration of 1 µg/ml.
A 1 hour primary incubation is recommended for this product. Preliminary experiments in human tonsil, thymus and peripheral mononucleocytes lysates gave no specific signal but low background. |
| 特記事項 |
|---|
|
Flow Cyt (Intra)
Use a concentration of 10 µg/ml. |
|
WB
Use a concentration of 1 µg/ml. A 1 hour primary incubation is recommended for this product. Preliminary experiments in human tonsil, thymus and peripheral mononucleocytes lysates gave no specific signal but low background. |
ターゲット情報
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機能
Inhibits the Ras signaling pathway through its intrinsic Ras GTPase-activating protein (GAP) activity. Acts as a negative feedback inhibitor of the calcineurin signaling pathway that also mediates crosstalk between calcineurin and Ras. -
組織特異性
Most abundant in spleen and peripheral blood leukocytes. -
配列類似性
Contains 1 Rab-GAP TBC domain. - Information by UniProt
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参照データベース
- Entrez Gene: 610509 Dog
- Entrez Gene: 374403 Human
- Entrez Gene: 108995 Mouse
- Entrez Gene: 361697 Rat
- Omim: 610831 Human
- SwissProt: Q8IV04 Human
- SwissProt: Q8C9V1 Mouse
- Unigene: 534648 Human
see all -
別名
- 1810062O14Rik antibody
- AI428527 antibody
- Carabin antibody
see all
画像
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Flow Cytometry (Intracellular) - Anti-carabin antibody (ab77625)
Flow cytometric analysis of paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton. Primary incubation with ab77625 for 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (1 μg/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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Western blot - Anti-carabin antibody (ab77625)All lanes : Anti-carabin antibody (ab77625) at 2 µg/ml
Lane 1 : Human Spleen
Lane 2 : Mouse thymus
Lysates/proteins at 35 µg per lane. -
Western blot - Anti-carabin antibody (ab77625)Lanes 1 & 3 : Anti-carabin antibody (ab77625) at 0.5 µg/ml
Lane 2 : Anti-carabin antibody (ab77625) at 0.3 µg/ml
Lane 1 : Jurkat cell lysate
Lane 2 : PBM cell lysates
Lane 3 : A431 cell lysate (negative control)
Lysates/proteins at 35 µg per lane. -
Western blot - Anti-carabin antibody (ab77625)Anti-carabin antibody (ab77625) at 2 µg/ml + Human Spleen lysate in RIPA buffer at 35 µg
Observed band size: 55 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab77625 は 3 報の論文で使用されています。
- Villagomez FR et al. TBC1D10C is a cytoskeletal functional linker that modulates cell spreading and phagocytosis in macrophages. Sci Rep 11:20946 (2021). PubMed: 34686741
- Zhu X et al. Cardiac-Specific EPI64C Blunts Pressure Overload-Induced Cardiac Hypertrophy. Hypertension 67:866-77 (2016). PubMed: 27021007
- Schickel JN et al. Carabin deficiency in B cells increases BCR-TLR9 costimulation-induced autoimmunity. EMBO Mol Med 4:1261-75 (2012). WB ; Mouse . PubMed: 23109291